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The effect of GA3, NAA, and KNO3 on in-vitro seed germination and plantlets performance of Highland Bamboo (Arundinaria alpina), South-West Ethiopia

Research Paper | March 20, 2022

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Belete Getnet, Yigardu Mulatu, Smegnew Melese, Marshet Nigatu

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J. Bio. Env. Sci.20( 3), 33-41, March 2022


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Propagation through seed is commonly used and cost effective to propagate tree plants if it is available and does not has different germination and shoot emergence difficulties. However, Arundinaria alpina seed has low germination performance and short storage period due to its mid recalcitrant behavior. Therefore, this research was initiated to evaluate the effects of pre-treatments on three months stored seed to improve germination and growth of shoots. The collected seeds were processed and brought to the laboratory. The analysis of variance revealed that the pretreatment factors NAA, GA3, and KNO3 were very highly significant (P ≤ 0.001) for all studied parameters except NAA was highly significant(P ≤ 0.01) for shoot number, and also KNO3 at shoot number and root length. However, KNO3 was non-significant at root number, GP, and PV. In this result, GA3 gave the highest (30%) germination percentage and shoot number at 8% and then followed by 23% at 20%, whereas NAA gave the highest (23%) at 6%, followed by 22% at 8%, but there was no somehow effective on KNO3 treatment. the lowest (10% & 5%) germination percentage was recorded at 10% GA3 and 20% NAA, respectively. In GA3, the highest shoot number, average of shoot length and root length were recorded at 8% whereas NAA produced the highest average root number and length at 8%, and average shoot length at 10% and 20%, but at 6% for shoot number. This concluded that the use of GA3 or NAA as a pre-treatment to enhance the germination and shoot quality of A. alpina seed is recommended. Also, surface disinfection should be practiced to avoid infections.


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The effect of GA3, NAA, and KNO3 on in-vitro seed germination and plantlets performance of Highland Bamboo (Arundinaria alpina), South-West Ethiopia

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