The effect of rhizobium inoculation with nitrogen fertilizer on growth and yield of soybeans (Glycine max L.)

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Research Paper 01/03/2017
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The effect of rhizobium inoculation with nitrogen fertilizer on growth and yield of soybeans (Glycine max L.)

M. S. Ntambo, Isaac Sindazi Chilinda, Aid Taruvinga, Sumaira Hafeez, Toheed Anwar, Rahat Sharif, Consolatha Chambi, Larry Kies
Int. J. Biosci.10( 3), 163-172, March 2017.
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The greatest success in terms of modified agricultural practices arising from scientific research on biological nitrogen fixation (BNF) has certainly been the development of rhizobial inoculants. Soybean (Glycine max L.)  has been the only widely adopted by farmers, mainly due to the relative specificity of the plant for rhizobia. Soybeans demand high amount of nitrogen, which are mainly obtained from biological nitrogen fixation.  In view of this, field experiment was conducted to evaluate the response of soybean (SC Siesta) to Bradyrhizobium japonicum inoculation and nitrogen fertilizer applied at four rates (0, 50, 100 and 200 kg N (34.5%) ha-1)). The experiment was laid out in randomized complete block design with three replications during 2015 – 2016 growing season at Africa University Farm, Mutare, Zimbabwe. Plant height, nodulation and nodule dry weight, pods number and pods dry weight, stem dry weight and yield were measured. Increasing nitrogen fertilizer in non-inoculated plants decreased nodule number and nodule dry weight but enhanced plant height, the pods number and pod dry weight, stem dry weight and grain yield. In the inoculated seeds, the application of 50 kg N ha-1increased plant height, pods number, pods dry weight and soybean yield which were as much as the application of 200 kg N ha-1 for the non-inoculated plants. Thus, on the obtained results the study suggests applying nitrogen fertilizer at the rate of 200 kg N (34.5%)ha-1 + non-inoculation or 50 kg N (34.5%)ha-1 fertilizer + inoculation of soybean seeds under field conditions in Mutare, Zimbabwe.


Ali A, Khan SA, Khan E, Ali N, Hussain I, Ahmad F. 2015. Genetic studies among diverse soybean (Glycine max L. Merrill) genotypes for variability and correlation at Swat. International Journal of Biosciences 6(4), 165-169.

Alam F, Bhuiyan M,Alam SS, WaghmodeTR, Kim PJ, Lee YB. 2015. Effect of Rhizobium sp. BARIRGm901 inoculation on nodulation, nitrogen fixation and yield of soybean (Glycine max) genotypes in gray terrace soil. Bioscience, Biotechnology and Biochemistry 79(10), 1660-1668.

Albareda M, Rodríguez-Navarro DN,  Temprano FJ. 2009. Soybean inoculation: Dose, N fertilizer supplementation and rhizobia persistence in soil. Field Crops Research 113(4), 352-356.

Argaw A. 2012. Evaluation of co-inoculation of Bradyrhizobium japonicum and phosphate solubilizing Pseudomonas spp. effect on soybean (Glycine max L. (Merr.)) in Assossa area. Journal of Agricultural Science andTechnology 14(1), 213-224.

Board JE. 2013. A Comprehensive survey of international soybean research-genetics, physiology, Agronomy and Nitrogen Relationships.Ana Pantar, Croatia, 1-624 p.

Catroux G, Hartmann A, Revellin C. 2001. Trends in rhizobial inoculant production and use. Plant and Soil 230(1), 21-30.

Elkoca E, Kantar F, Sahin F. 2007. Influence of nitrogen fixing and phosphorus solubilizing bacteria on the nodulation, plant growth and yield of chickpea. Journal of Plant Nutrition 31(1), 157-171.

El-ShaarawiAFI, Sabh AZ, Abou-Taleb SM, Ghonien AE. 2011. Effect of inorganic nitrogen and Bradyrhizobium japonicum inoculation on growth and yield of soybean. Astralian Journal of Basic Applied Science 5(10), 436-447.

Hungria M,Franchini JC,Campo RJ, Crispino CC, Moraes JZ, Sibaldelli RN, Mendes IC, Arihara J. 2006. Nitrogen nutrition of soybean in Brazil: contributions of biological N2 fixation and N fertilizer to grain yield. Canadian Journalof Plant Science 86(4), 927-939.

Hungria M, Nogueira MA, Araujo RS. 2015. Soybean seed co-inoculated with Bradyrhizobium spp. and Azospirillum brasilense. A new biotechnological tool improve yield and sustainability. American Journal ofPlant Science 06(06), 811-817.

Jacob J, Lawlor DW. 1992. Dependence of photosynthesis of sunflower and maize leaves on phosphate supply, ribulose-1, 5-bisphosphate carboxylase/oxygenase activity, and ribulose-1, 5-bisphosphate pool size. Plant Physiology 98(3), 801-807. http:/​/​dx.​doi.​org/​10.​1104/​pp.​98.​3.​801

Janagard MS, Ebadi-Segherloo A. 2016. Inoculated soybean response to starter nitrogen in conventional cropping system in Moghan. Journal of Agronomy 15(1), 26-32.

Javaid A, Anjum T, Bajwa R. 2002. EM and VAM technology in Pakistan. XII: Growth, nodulation and VA mycorrhizal response of Phaseolas vulgaris to long-term EM application. Pakistan Journal of Phytopathology 14(1), 57-61.

Kala TC, Christi RM, Bai NR. 2011. Effect of Rhizobium inoculation on the growth and yield of horsegram (Dolichos biflorus Linn). Plant Archives, 11(1), 97-99.

Mengel D, Diaz DR. 2012. Applying nitrogen to N-stressed soybeans.

MbahEU, Muoneke CO, Okpara DA. 2007. Effect of compound fertilizer on the yield and productivity of soybean and maize in soybean/maize intercrop in southeastern Nigeria. Tropical and Subtropical  Agroecosystems 7(2), 87-95.

Miles M, Levy C, Hartman G. 2004. Summary of the USDA fungicide efficacy trials to control soybean rust in Zimbabwe 2003-2004. Integrated Pest Management Reviews.

Mishra PR, Mishra S, Selvakumar G, Bisht JK, Kundu S, Gupta HS. 2009. Coinoculation of Bacillus thuringeinsis -KRI with Rhizobium leguminosarum enhances plant growth and nodulation of pea (Pisum sativum L.) and lentil (Lens culinaris L.). World Journal of Microbiology andBiotechnology 25(5), 753-761.

Mohamed AA, Hassan MA. 2015. Evaluation  of two chickpea (Cicer arientinun L.) cultivars in response to three Rhizobium strains at River Nile State, Sudan. Merit Research Journal of Agricultural Science and Soil Science 3(5), 062-069.

Nyamapfene KW. 1991. The soils of Zimbabwe. Nehanda Publishers.179 P.

Ntambo MS, Manyangarirwa W, Taurira M, Kankonda OM. 2015. Effect of the lepidoptera stem borers, Busseola fusca (Fuller) and Chilo partellus (Swinhoe) on green mealies production. Internal Journal of Innovative Research and Development, 4(10), 366- 374.

Rahman M, Bhuiyan M,Sutradhar G,Rahman M, Paul A. 2008. Effect of phosphorus, molybdenum and rhizobium inoculation on yield and yield attributes of mungbean. International Journal of Sustainable Crop Production 3(6), 26-33.

Salih SH, Hamd SAM, Dagash YMI. 2015. The effects of rhizobium, mycorrhizal inoculations and diammonium phosphate (DAP) on nodulation, growth and yield of soybean. Universal Journal of Agricultural Research 3(1), 11-14.

Seneviratne G, Van Holm LHJ, Ekanayake EMHGS. 2000. Agronomic benefits of rhizobial inoculant use over nitrogen fertilizer application in tropical soybean. Field Crops Research 68(3), 199-203.

Soe KM, Bhromsiri A, Karladee D. 2010. Effect of selected endophytic actinomycetes (Streptomyces sp.) and bradyrhizobial from Myanmar on growth, nodulation, nitrogen fixation and yield of different soybean varities. Msc thesis, Chiang Mai University, Thailand.

Solaiman ARM, Hussain D. 2006. Effectiveness of Bradyzhizobium japonicum strains on soybean at field condition. Bulletin of the Institute of Tropical  Agriculture, Kyushu University 29(1), 11-20.

Starling ME, Wood CW, Weaver DB. 1998. Starter nitrogen and growth habit effects on late- planted soybeans respond to nitrogen starter. Agronomy Journal 90, 658-662.

Sheaffer CC, Moncada KM, Sequin P. 2009. Crop profiles : Legumes. In: Introduction to Agronomy: Food, Crops and Environment, Sheaffer CC and Moncana KK (Eds.)., Delmar Cengage Learning, ISBN: 1-4180-5037-7, Clifton Park, 580-602 P.

Tahir MM, Abbasi MK, Rahim N, Khaliq A, Kazmi MH. 2009. Effect of Rhizobium inoculation and NP fertilization on growth, yield and nodulation of soybean (Glycine max L.) in the sub-humid hilly region of Rawalakot Azad Jammu and Kashmir, Pakistan. African Journal of Biotechnology 8(22), 6191-6200.

Tirichine L, James EK, Sandal N, Stougaard J. 2006. Spontaneous root-nodule formation in the model legume Lotus japonicus: a novel class of mutants nodulates in the absence of rhizobia. Molecular Plant- Microbe Interactions Journal 19(4), 373-382.

Tran VT, Lien TTP, Mau CH. 2015. Proteomic characterization of soybean leaf proteins from cultivar DT2000 with rust-resistant ability in Vietnam. International Journal of Biosciences 6(2), 468-477.

Usman M, Nangere MG, Musa I. 2015. Effect of three levels of NPK fertilizer on growth parameters and yield of maize-soybean intercrop. Internal  Journal of Scientific Research Publications 5(9), 2250-3153.

Wilcox JR, Shibles RM. 2001. Interrelationships  among seed quality attributes in soybean. Crop Science 41(1), 11-14.

Zuffo AM, Rezende PM, Bruzi AT, Oliveira NT, Soares IO, Geraldo FGN, Bruno ESC, Silva LO. 2015. Co-inoculation of Bradyrhizobium japonicum and. Azospirillum brasilense in the soybean crop. Revista Brasileira de Ciências Agrárias 38(1), 87-93.