The effects of thermal, osmotic and acid stress on Lactobacillus plantarum and Lactobacillus brevis

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Research Paper 01/01/2018
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The effects of thermal, osmotic and acid stress on Lactobacillus plantarum and Lactobacillus brevis

Boublenza Faiza, Baghdad Belhadj Fatima Zohra, Zadi Karam Halima, Karam Nour Eddine
Int. J. Biosci.12( 1), 51-64, January 2018.
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Abstract

Adaptation to environmental stress is an essential process for bacterial survival and growth. The aim of this work is to study the behavior of two Lactobacillus strains in osmotic, thermal or acid constraints with a view to a possible technological application. The degree of tolerance conferred on Lactobacillus plantarum and Lactobacillus brevis, by an osmotic, thermal and acid shock was evaluated, survivors cells were estimated at time 0 and after different times of incubation of stress challenge; the absorbance (OD600nm) values were determined at the same intervals. The lethal treatment was calculated (CFU at T0 – CFU at T) /CFU at T0. During hyper osmotic stress, growth capacities are reduced, the acid and thermal stress also decreases the survival cells; at pH=1.5 and 2.5, the survival is strongly affected (absence of viable and cultivable cells from 72 h of incubation). After adaptation of the bacteria to the different stresses, growth and survival are significantly improved, at 2.5% NaCl, 18 ° C and 37 ° C, pH 4.5 and 5.5, the values obtained are similar to those obtained under the favorable conditions of growth. The study of the mortality rate indicates that cells are resistant or not to varying stress degrees from strain and from one stress intensity to another more important .The adaptation of the cells has a positive effect but only at reduced incubation times( optimal tolerance factors). This knowledge is essential in order to promote the proliferation and / or survival of these bacteria used as leaven in hostile environment.

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Baliarda A, Robert H, Jebbar M, Blanco C, Deschamps A, Le Marrec C. 2003. Potential osmoprotectants for the lactic acid bacteria Pediococcus pentosaceus and Tetragenococcus halophila. International Journal of Food Microbiology 84, 13–20.

Belkheir K, Centeno JA, Zadi-Karam H, Karam NE, Carballo J. 2016. Potential technological interest of indigenous lactic acid bacteriafrom algerian camel milk. Italian Journal of Food Science 28, 598-611. www.researchgate.net/publication/309807328

Besnard V, Federighi M, Cappelier JM. 2000. Development of a direct viable count procedure for the investigation of VBNC state in Listeria monocytogenes. Letters in Applied Microbiology 31, 77-81.

Bourlioux P, Koletzko B, Guarner F, Braesco V. 2007. The intestine and its microflora are partners for the protection of the host: report on the Danone Symposium “The Intelligent Intestine”. The American Journal of Clinical Nutrition 78(4), 675-683.

Buck  BL, Azcarate-Peril MA, Klaenhammer TR. 2009. Role of autoinducer-2 on the adhesion ability of Lactobacillus acidophilus. Journal of applied Microbiology 107, 269-279. http://dx.doi.org/10.1111/j.1365-2672.2009.04204.x

Di Cango R, De Angelis M, Limitone A, Fox P F, Gobbetti M. 2006. Response of Lactobacillus helveticus PR4 to heat stress during propagation in cheese whey with a gradient of decreasing temperatures. Applied and Environmental Microbiology 72(7), 4503-4514. https://dx.doi.org/10.1128%2FAEM.01829-05

Essaid I, Medini M, Hassouna M. 2009. Technological and safety proprieties of Lactobacillus plantarum strains isolated from a Tunisian traditional salted meat. Meat Science 81, 203-208. http://dx.doi.org/10.1016/j.meatsci.2008.07.020

Flahaut S, Hartke A, Giard JC, Benachour A, Boutibonnes P, Auffray Y. 1996. Relationship between stress response toward bile salts, acid and heat treatment in Enterococcus aecalis. FEMS Microbiology Letters 138, 49–54.

Garbay S, Lonvaud-Funel A. 1996. Response of Leucostoc oenos to environmental changes. Journal of Applied Bacteriology 81,619-625.

Guillouard I, Lim EM, Van de Guchte M, Grimaldi C, Penaud S, Maguin E. 2004. Tolerance and adaptative acid stress response of Lactobacillus delbrueckii ssp. bulgaricus. Le Lait 84, 1-6.

Gwenola G, Gwenaël J, Patrick B. 2001. Lactobacillus delbrueckii ssp. bulgaricus thermotolerance. Le Lait 81, 301–309. http://dx.doi.org/10.1051/lait:2001133

Hutkins RW, Nannen NL. 1993. pH homeostasis  in  lactic acid bacteria. Journal of Dairy Science 76(8), 2354-2365. www.dx.doi.org/10.3168/jds.S00220302(93)77573-6

Jobin MP, Delmas F, Guzzo J. 1998.Caractérisation des protéines de choc thermique de faible poids moléculaire chez les bactéries lactiques. Lait 78, 165-171.

 Kim WS, Park JH, Ren J, Su P, Dunn WN. 2001. Survival response and rearrangement of plasmid DNA of Lactococcus lactis during long-term starvation. Applied and Environmental Microbiology 67(10), 4594-4602. www.dx.doi.org/10.1128%2FAEM.67.10.45944602.2001

Lorca GL, Font de Valdez G. 1999.The effect of suboptimal growth temperature and phase on resistance of Lactobacillus acidophilus to environmental stress. Cryobiology 39, 144-153. https://doi.org/10.1006/cryo.1999.2193

Lorca G, Font de Valdez L. 2011. Alow pH inducible,Stationary phase acid tolerance response in Lactobacillus acidophilus CRL 639. Current Microbiology 42, 21-26. https://doi.org/10.1007/s002840010172

Marceau A, Zagorec M, Champomier-Verges MC. 2003. Positive effects of growth at suboptimal temperature and high salt concentration on long-term survival of Lactobacillus sakei. Research in Microbiology 154, 37-42. https://doi.org/10.1016/S0923-2508(02)00010-4.

Murga ML, De Ruiz Holgado AP, De Valdez GF. 1998Survival rate and enzyme activities of Lactobacillus acidophilus following frozen storage. Cryobiology 36(4), 315-319.

O’Sullivan E, Condon S. 1999. Relationship  between  acid  tolerance,  cytoplasmic  pH,  and ATP  and H+-ATPase  levels  in  chemostat  cultures  of Lactococcus  lactis. Applied and Environmental Microbiology 65,2287-2380.

Özer B, Kirmaci HA, Şenel E, Atmar M, Hayaloğlu A. 2009. Improving the viability of Bifiodobacterium bifidum BB-12 and Lactobacillus acidophilus LA-5 in white-brined cheese by microencapsulation. International Dairy Journal 19, 22-29.

Palmfeldt J, Hahn-Hägerdal B. 2000. Influence of culture pH on survival of Lactobacillus reuteri subjected to freeze-drying. International Journal of Food Microbiology 55,235-238. http://dx.doi.org/10.1016/S0168-1605(00)00176-8

Prasad J, Mc Jarrow P, Gopal P. 2003. Heat and osmotic stress responses of probiotic Lactobacillus rhamnosus HN001 (DR20) in relation to viability after drying. Applied and  Environmental Microbiology 69, 917-942. www.dx.doi.org/10.1128%2FAEM.69.2.917925.2003.

Silva J, Carvalho AS, Ferreira R, Vitorino R, Amado F, Domingues P, Teixeira P, Gibbs PA. 2005. Effect of the pH of growth on the survival of Lactobacillus delbrueckii subsp. bulgaricus to stress conditions during spray drying. Journal of Applied Microbiology 98, 775-782.

Wang Y, Delettre J, Guillot A, Corrieu G,Béal C. 2005Influence of cooling temperature  and  duration  on  cold  adaptation of Lactobacillus  acidophilus  RD758. Cryobiology 50, 294-307.

Zotta T, Ricciardi A, Ciocia F, Rossano R, Parente E. 2008. Diversity of stress responses in dairy thermophilic Streptococci. International Journal of Food Microbiology 124, 34–42. www.doi.org/10.1016/j.ijfoodmicro.2008.02.024