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The effects of thermal, osmotic and acid stress on Lactobacillus plantarum and Lactobacillus brevis

Research Paper | January 1, 2018

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Boublenza Faiza, Baghdad Belhadj Fatima Zohra, Zadi Karam Halima, Karam Nour Eddine

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Int. J. Biosci.12( 1), 51-64, January 2018

DOI: http://dx.doi.org/10.12692/ijb/12.1.51-64


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Adaptation to environmental stress is an essential process for bacterial survival and growth. The aim of this work is to study the behavior of two Lactobacillus strains in osmotic, thermal or acid constraints with a view to a possible technological application. The degree of tolerance conferred on Lactobacillus plantarum and Lactobacillus brevis, by an osmotic, thermal and acid shock was evaluated, survivors cells were estimated at time 0 and after different times of incubation of stress challenge; the absorbance (OD600nm) values were determined at the same intervals. The lethal treatment was calculated (CFU at T0 – CFU at T) /CFU at T0. During hyper osmotic stress, growth capacities are reduced, the acid and thermal stress also decreases the survival cells; at pH=1.5 and 2.5, the survival is strongly affected (absence of viable and cultivable cells from 72 h of incubation). After adaptation of the bacteria to the different stresses, growth and survival are significantly improved, at 2.5% NaCl, 18 ° C and 37 ° C, pH 4.5 and 5.5, the values obtained are similar to those obtained under the favorable conditions of growth. The study of the mortality rate indicates that cells are resistant or not to varying stress degrees from strain and from one stress intensity to another more important .The adaptation of the cells has a positive effect but only at reduced incubation times( optimal tolerance factors). This knowledge is essential in order to promote the proliferation and / or survival of these bacteria used as leaven in hostile environment.


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