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The immune modulatory activity of lectin extracted from the Algerian Castanea crenata

Imen Torche, Youcef Necib, Ahlem Bahi, Sana Boufker

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Int. J. Biosci.21(5), 326-334, November 2022

DOI: http://dx.doi.org/10.12692/ijb/21.5.326-334

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Abstract

A lectin has been purified from the Algerian chestnut seeds (Castanea crenata), one of the natural biosourced plants in Algeria. This lectin extracted from the inner shell of the seeds exhibited a high hemagglutinating activity toward rabbit and human erythrocytes, and remained stable under a wide range of pH levels 2-12 and temperatures up to 120 °C. The agglutinating activity was inhibited by glucose, N-acétyl-glusamine, Saccharine sodique but more by mannose also by BSA, fetuin, Ovalbumin, mucin. The extracted lectin from Castanea crenata found to be non-metal binding lectin when tested against several metals. In the last part of this study, The immunomodulatory potential effect of Castanea crenata’ s inner shell lectin on the phagocytic activity was measured by the carbon clearance rate test, at different doses (30, 50, and100mg/kg), where the administration of the extracted lectin intravenously in mice increases significantly the phagocytic index K comparing to control, especially with high doses, which enhances the clearance rate of carbon in blood indicating the stimulation of the reticulo-endothelial system. Thus, the present study reveals that Castanea crenata’s inner shell lectin holds promise as immunomodulatory agent, which acts by stimulating dose dependent phagocytic function.

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The immune modulatory activity of lectin extracted from the Algerian Castanea crenata

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