The impact of biofilm on the colonization between Staphylococcus aureus and Mycoplasma bovis under in-vitro conditions
Paper Details
The impact of biofilm on the colonization between Staphylococcus aureus and Mycoplasma bovis under in-vitro conditions
Abstract
Mastitis is a major cause of decrease in milk production in cattle and buffaloes that leads to the economic losses to the dairy industry. Staphylococcus aureus and Mycoplasma bovis both play an important role in causation of mastitis. Biofilm formation property of these bacteria helped to understand the pathogen city of mastitis in bovine. Twenty milk sample of each sub clinical and clinical mastitis were collected, Staphylococcus aureu sand Mycoplasma bovis were isolated and their biofilm was developed by cover slip method under in-vitro conditions and then they were treated with homologous and heterogonous culture isolates for the cross colonization potential. The homologous biofilm for S. aureus and M. bovis had resulted into significant increase in growth (CFU) with mean value of 599.8/HPF and 558.8/HPF respectively, while the heterogonous growth of S. aureus and M. bovis were having mean values of 548.8/HPF and 181.6/HPF respectively. The growth potential of S. aureus was equally high irrespective of biofilm source whereas the biofilm of S. aureus rarely promoted the growth of M. bovis. The overall results proved that the initial M. bovis infection may have the potential to promote S. aureusas secondary infections with equal intensity. In future more mastitis pathogens may also be studied in similar pattern resolved the pathogen city under heterogonous environment.
Ashfaq M, Ghulam M, Shamsheer H, Amar R. 2015. Effects of livestock diseases on dairy production and incomes in district Faisalabad, Punjab, Pakistan.Working Paper No. 023, 1-31.
Bradley AJ. 2002. Bovine mastitis: An evolving disease. Veterinary Journal 164, 116-128.
Chima JC, Ojo MO, Molokwu JU, Okewole PA. 1995. Characterization of Mycoplasmas isolated from genital tract infections of sheep in Nigeria. International office Epizootics 14, 865-871.
Fox LK, Zadoks RN, Gaskins CT. 2005. Biofilm production by Staphylococcus aureus associated with intramammary infection. Veterinary Microbiology 107, 295-299.
Ghadersohi A, Hirst RG, Forbes FJ, Coelen RJ. 1999. Preliminary studies on the prevalence of Mycoplasma bovis mastitis in dairy cattle in Australia. Veterinary Microbiology 65, 185-194.
Ibrahim IA, Badaalambedji R, Duprez J, Djika M. 2013. Bacterial mastitis in the Azawak zebu breed at the Sahelian experimental station in Toukounous, identification and typing of Staphylococcus aureus. International Research Journal of Microbiology 4, 168-178.
Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. 2006. Detection of biofilm formation among the clinical isolates of Staphylococci: an evaluation of three different screening methods. Indian Journal of Medical Microbiology 24, 25-69.
Mcauliffe L, Ellis RJ, Miles K, Ayling RD, Nicholas RAJ. 2006. Biofilm formation by Mycoplasma species and its role in environmental persistence and survival. Microbiology 152, 913-922.
Melchior MB, Vaarkamp H, Fink-Gremmels J. 2006. Biofilms a role in recurrent mastitis infections. Veterinary Journal 171, 398-407.
Pfutzner H, Sachse K. 1996. Mycoplasma bovis as an agent of mastitis, pneumonia, arthritis and genital disorders in cattle. Revue Scientifiqueet technique (office International Epizootics) 15, 1477-1494.
Pyorala S, Taponen S. 2009. Coagulase negative staphylococci emerging mastitis pathogens. Veterinary Microbiology 134, 3-8.
Radaelli E, Castiglioni V, Losa M, Benedetti V, Piccinini R, Nicholas RAJ, Luini M. 2011. Outbreak of bovine clinical mastitis caused by Mycoplasma bovis in a North Italian herd. Research in Veterinary Science 91, 251-253.
Shivakumar V, Chakravortty D. 2014. Biofilms community behavior by bacteria. Resonance 1005-1016.
Shrihari RY. 2011. Detection, Identification and Characterization of Staphylococci in Street Vend Foods characterization of Staphylococcus Isolates. Food and Nutrition Science 02, 304-313.
Simmons WL, Dybvig K. 2003. The Vsa proteins modulate susceptibility of Mycoplasma pulmonis to complement killing, heamadsorption, and adherence to polystyrene. Infections and Immunology 71, 5733-5738.
Speziale P, Pietrocola G, Foster TJ, Geoghegan JA. 2014. Protein based biofilm matrices Staphylococci. Frontiers in Cellular and Infectious Microbiology 4, 1-10.
Taj Y, Essa F, Aziz F, Kazmi SU. 2012. Original Article Study on biofilm forming properties of clinical isolates of Staphylococcus aureus. Journal of Infection in Developing Countries 5, 403-409.
Ahsan Naveed, Sajjad-ur-Rahman, M. Imran Arshad, Faisal Rasheed Anjum, Khurram Ashfaq, M. Farooque Hassan, Qamar Majeed, Sadaf Naz, 2017. The impact of biofilm on the colonization between Staphylococcus aureus and Mycoplasma bovis under in-vitro conditions. Int. J. Biosci., 10(5), 389-397.
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