The impact of biofilm on the colonization between Staphylococcus aureus and Mycoplasma bovis under in-vitro conditions

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Research Paper 01/05/2017
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The impact of biofilm on the colonization between Staphylococcus aureus and Mycoplasma bovis under in-vitro conditions

Ahsan Naveed, Sajjad-ur-Rahman, M. Imran Arshad, Faisal Rasheed Anjum, Khurram Ashfaq, M. Farooque Hassan, Qamar Majeed, Sadaf Naz
Int. J. Biosci.10( 5), 389-397, May 2017.
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Mastitis is a major cause of decrease in milk production in cattle and buffaloes that leads to the economic losses to the dairy industry. Staphylococcus aureus and Mycoplasma bovis both play an important role in causation of mastitis. Biofilm formation property of these bacteria helped to understand the pathogen city of mastitis in bovine. Twenty milk sample of each sub clinical and clinical mastitis were collected, Staphylococcus aureu sand Mycoplasma bovis were isolated and their biofilm was developed by cover slip method under in-vitro conditions and then they were treated with homologous and heterogonous culture isolates for the cross colonization potential. The homologous biofilm for S. aureus and M. bovis had resulted into significant increase in growth (CFU) with mean value of 599.8/HPF and 558.8/HPF respectively, while the heterogonous growth of S. aureus and M. bovis were having mean values of 548.8/HPF and 181.6/HPF respectively. The growth potential of S. aureus was equally high irrespective of biofilm source whereas the biofilm of S. aureus rarely promoted the growth of M. bovis. The overall results proved that the initial M. bovis infection may have the potential to promote S. aureusas secondary infections with equal intensity. In future more mastitis pathogens may also be studied in similar pattern resolved the pathogen city under heterogonous environment.


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