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The influence of pH on oil dispersant toxicity to the whiteleg shrimp, Litopenaeus vannamei

Research Paper | June 1, 2017

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Muhammad Arif Asadi, Ahmad Didin Khoiruddin, Anthon Andrimida

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J. Bio. Env. Sci.10( 6), 201-208, June 2017


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Oil spillage accidents lead the use of oil dispersants to break oil into small droplets to prevent oil coming ashore. Meanwhile, recent and rapid drop in surface pH could have devastating consequences to marine environment. However, their combined effects on marine species have not been experimentally evaluated. This study evaluated the toxicity of oil dispersant in the shrimp Litopenaeus vannamei under pH 6.5 and 8.5. Healthy post larvae L. vannamei were exposed to dispersant solution at concentration of 0%, 3%, 6%, 12% and 24% for 72 hours to determine mortality. Probit analysis was used to determine LC50, while the PAH of dispersant solution was characterized using GCMS method. The dispersant had negative effects to L. vannamei and that toxicity of dispersant increased over time and exposure concentration. The 72-h LC50 were equivalent to 191.18 mgL-1 and 553 mgL-1 for pH 6.5 and 8.5 respectively in which that dispersant is practically non-toxic to the shrimps. However, the combination of dispersant and lower pH increases the mortality of the shrimp; thus ocean acidification may increase dispersant accumulation in the L. vannamei tissue via surface contacting.


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The influence of pH on oil dispersant toxicity to the whiteleg shrimp, Litopenaeus vannamei

Adams J, Sweezey M, Hodson PV. 2014. Oil and oil dispersant do not cause synergistic toxicity to fish embryos. Environmental Toxicology and Chemistry33, 107–114. http://dx.doi.org/10.1002/etc.2397

Almeda R, Hyatt C, Buskey EJ. 2014. Toxicity of dispersant Corexit 9500A and crude oil to marine microzooplankton. Ecotoxicology and Environmental Safety106, 76–85. https://doi.org/10.1016/j.ecoenv.2014.04.028

Bergey LL, Weis JS.2007. Molting as a mechanism of depuration of metals in the fiddler crab, Ucapugnax. Marine Environmental Research64, 556–562. https://doi.org/10.1016/j.marenvres.2007.04.009

Carls MG, Holland L, Pihl E, Zaleski MA, Moran J, Rice SD. 2016. Polynuclear Aromatic Hydrocarbons in Port Valdez Shrimp and Sediment. Archives of Environmental Contamination and Toxicology 71, 48–59. https://doi.org/10.1007/s00244-016-0279-3

Chen K, Li E, Gan L, Wang X, Xu C, Lin H, Qin JG, Chen L. 2014. Growth and Lipid Metabolism of the Pacific White Shrimp Litopenaeus vannamei at Different Salinities. Journal of Shellfish Research33, 825–832. https://doi.org/10.2983/035.033.0317

Daly KL, Passow U, Chanton J, Hollander D.2016. Assessing the impacts of oil-associated marine snow formation and sedimentation during and after the Deepwater Horizon oil spill. Anthropocene 13, 18–33. https://doi.org/10.1016/j.ancene.2016.01.006

Furtado PS, Fugimura MMS, Monserrat JM, Souza DM, Garcia LDO, Wasielesky W.2015. Acute effects of extreme pH and its influences on the survival and biochemical biomarkers of juvenile White Shrimp, Litopenaeus vannamei. Marine and Freshwater Behaviour and Physiology 48, 417–429. https://doi.org/10.1080/10236244.2015.1086539

GaoW, Tian L, Huang T, Yao M, Hu W, Xu Q. 2016. Effect of salinity on the growth performance, osmolarity and metabolism-related gene expression in white shrimp Litopenaeus vannamei. Aquaculture Reports4, 125–129. https://doi.org/10.1016/j.aqrep.2016.09.001

Garr A, Laramore S, Krebs W. 2014. Toxic Effects of Oil and Dispersant on Marine Microalgae. Bull. Bulletin of Environmental Contamination and Toxicology93, 654–659. https://doi.org/10.1007/s00128-014-1395-2

George-Ares A, Clark JR, Biddinger GR, Hinman ML. 1999. Comparison of Test Methods and Early Toxicity Characterization for Five Dispersants. Ecotoxicology and Environmental Safety42, 138–142. https://doi.org/10.1006/eesa.1998.1734

Hook SE, Osborn HL.2012. Comparison of toxicity and transcriptomic profiles in a diatom exposed to oil, dispersants, dispersed oil. Aquatic Toxicology124–125, 139–151. https://doi.org/10.1016/j.aquatox.2012.08.005

Hu M, Lin D, Shang Y, Hu Y, Lu W, Huang X, Ning K, Chen Y, Wang Y. 2017. CO2-induced pH reduction increases physiological toxicity of nano-TiO2 in the mussel Mytiluscoruscus. Nature Scientific Reports 7, 40015. https://doi.org/10.1038/srep40015

Jiang Z, Huang Y, Xu X, Liao Y, Shou L, Liu J, Chen Q, Zeng J. 2010. Advance in the toxic effects of petroleum water accommodated fraction on marine plankton. ActaEcologicaSinica30, 8–15. https://doi.org/10.1016/j.chnaes.2009.12.002

Lee KW, Shim WJ, Yim UH, Kang JH.2013. Acute and chronic toxicity study of the water accommodated fraction (WAF), chemically enhanced WAF (CEWAF) of crude oil and dispersant in the rock pool copepod Tigriopus japonicus. Chemosphere 92, 1161–1168. https://doi.org/10.1016/j.chemosphere.2013.01.080

Lessard R, DeMarco G. 2000. The Significance of Oil Spill Dispersants. Spill Science & Technology Bulletin6, 59–68. https://doi.org/10.1016/S1353-2561(99)00061-4

Lyons MC, Wong DKH, Mulder I, Lee K, Burridge LE.2011. The influence of water temperature on induced liver EROD activity in Atlantic cod (Gadusmorhua) exposed to crude oil and oil dispersants. Ecotoxicology and Environmental Safety74, 904–910. https://doi.org/10.1016/j.ecoenv.2010.12.013

Melzner F, Gutowska MA, Langenbuch M, Dupont S. Lucassen M, Thorndyke MC, Bleich M, Pörtner HO. 2009. Physiological basis for high CO2 tolerance in marine ectothermic animals: pre-adaptation through lifestyle and ontogeny?.Biogeosciences6, 2313–2331. https://doi.org/10.5194/bg-6-2313-2009

National Research Council (NRC). 2002. Oil in the sea III: Inputs, fates and effects. National Research Council, Washington DC.

Nizzetto L, Lohmann R, Gioia R, Jahnke A, Temme C, Dachs J, Herckes P, Guardo AD, Jones KC. 2008. PAHs in Air and Seawater along a North–South Atlantic Transect: Trends, Processes and Possible Sources. Environmental Science & Technology42, 1580–1585. https://doi.org/10.1021/es0717414

Orr JC, Fabry VJ, Aumont O, Bopp L, Doney SC, Feely RA, Gnanadesikan A, Gruber N, Ishida A, Joos F, Key RM, Lindsay K, Maier-Reimer E, Matear R, Monfray P, Mouchet A, Najjar RG, Plattner GK, Rodgers KB, Sabine CL, Sarmiento JL, Schlitzer R, Slater RD, Totterdell IJ, Weirig MF, Yamanaka Y, Yool A. 2005. Anthropogenic ocean acidification over the twenty-first century and its impact on calcifying organisms. Nature 437, 681–686. https://doi.org/10.1038/nature04095

Otitoloju AA. 2005. Crude oil plus dispersant: always a boon or bane? Ecotoxicology and Environmental Safety60, 198–202. https://doi.org/10.1016/j.ecoenv.2003.12.021

Rand G.1995. Fundamentals of aquatic toxicology : effects, environmental fate, and risk assessment. Taylor & Franchis, Washington DC.

Senanan W, Tangkrock-Olan N, Panutrakul S, Barnette P, Wongwiwatanawute C, Niphonkit N, Anderson DJ.2007. The presence of the Pacific Whiteleg Shrimp (Litopenaus vannamei, BOONE, 1931) in the wild in Thailand. Journal of shellfish research26, 1187–1192. https://doi.org/10.2983/07308000(2007)26[1187:TPOTPW]2.0.CO;2

Singer MM, Aurand DV, Coelho GM, Bragin GE, Clark JR, Sowby M, Tjeerdema RS. 2001. Making, measuring, and using water-accomodated fractions of petroleum for toxicity testing. International Oil Spill Conference Proceedings 2001, 1269–1274. https://doi.org/10.7901/2169-3358-2001-2-1269

Vieira LR, Guilhermino L.2012. Multiple stress effects on marine planktonic organisms: Influence of temperature on the toxicity of polycyclic aromatic hydrocarbons to Tetraselmis chuii. Estuaries in a Changing Climate72, 94–98. https://doi.org/10.1016/j.seares.2012.02.004

Wells PG, Abernethy S, Mackay D. 1982. Study of oil-water partitioning of a chemical dispersant using an acute bioassay with marine crustaceans. Chemosphere 11, 1071–1086. https://doi.org/10.1016/0045-6535(82)90112-6