The inheritance AT Xa27 Gene in F2 progenies of Rice crossed between ting gong and IRBB27

Paper Details

Research Paper 01/10/2018
Views (891)
current_issue_feature_image
publication_file

The inheritance AT Xa27 Gene in F2 progenies of Rice crossed between ting gong and IRBB27

Farid Fitrya, Bakhtiar Basyah, Sabaruddin Zakaria
Int. J. Agron. & Agric. Res. 13(4), 15-21, October 2018.
Copyright Statement: Copyright 2018; The Author(s).
License: CC BY-NC 4.0

Abstract

Ting gong is a local rice variety that is famous for its fluffy rice texture and good adaptability. Recently this variety rarely cultivated by farmers because its deep-aged and susceptible to bacterial bligh disease. Improved performance of this variety can be done through plant breeding programs needs to be carried out. One method that can be done is crossing by utilizing the introduced variety as the source of the target gene. This study aims to analyze F2 plants that carry the Xa27 gene and evaluate their agronomic aspects. The study was conducted in a screen house, Plant Disease Laboratory and Plant Breeding Laboratory, Faculty of Agriculture, Syiah Kuala University, from July 2017 to January 2018. The seeds used were F2 progenies from a crossing between IRBB27 and local variety Ting gong. Each pot is planted with two plants and there were 12 individual plants for analysis. Resistance test for Xanthomonas oryzae pv. oryzae and molecular analysis were carried out to identify the presence of target genes (Xa27) in F2 plants. The results showed that 91.66% of the plants showed resistant symptoms (R) against Xanthomonas oryzae pv. oryzae, and 8.3% plant shows mildly susceptible to bacterial blight (MS).

Abadi LA. 2003. Genetika Penyakit Tumbuhan dalam Ilmu Penyakit Tumbuhan 2. Fakultas Pertanian Universitas Brawijaya Bayumedia Publising Malang.

Amante-Bordeos A, Sitch LA, Nelson R,  Dalmacio RD, Oliva NP, Aswidinnoor H, Leung H. 1992. Transfer of bacterial blight and blast resistance from the tetraploid wild rice Oryza minuta to cultivated rice, Oryza sativa. Theoretical and Applied Genetics 84, 345–354.

Gnanamanickam SS, Priyadarisini VB, Narayanan NN, Vasudevan P, Kavitha S. 1999. An overview of bacterial blight disease of rice and strategies for its management. Curriculum Science. 77, 1435–1444.

Kadir TS. 2009. Menangkal HDB dengan menggilir varietas. Warta Penelitian Dan Pengembangan Pertanian. 31, 1–3.

Kauffman HE. 1973. An improved technique for evaluat-ing resistance of rice varieties to Xanthomonas oryzae. Plant Disease Rep. 57, 537–541.

Luo Y, Sangha JS, Wang S, Li Z, Yang J, Yin Z. 2012. Marker-assisted breeding of Xa4, Xa21 and Xa27 in the restorer lines of hybrid rice for broad-spectrum and enhanced disease resistance to bacterial blight. Moleculer Breeding. 30, 1601–1610.

Luo Y, Yin Z. 2013. Marker-assisted breeding of Thai fragrance rice for semi-dwarf phenotype, submergence tolerance and disease resistance to rice blast and bacterial blight. Moleculer Breeding 32, 709–721.

Mew TW,  Alvarez AM, Leach JE, Swings J. 1993. Focus on bacterial blight of rice. Plant Disease. 77, 5–12.

Nusifera S, Lestari AP, Alia Y.  2014. Penampilan dan parameter genetik beberapa karakter morfologi agronomi dari 26 aksesi padi (Oryza spp L.) lokal Jambi. Jurnal Penelitian Universitas Jambi Seri Sains. 16.

Ogawa T. 1993. Methods and strategy for   monitoring race distribution and identification of resistance genes to bacterial leaf blight (Xanthomonas campestris pv. oryzae) in rice [Oryza sativa]. JARQ Jpn.

Ogawa T, Busto GA, Tabien RE, Khush GS. 1988. Further study of Xa-4b gene for resistance to bacterial blight of rice. Rice Genet News. 5, 104–106.

Ou SH. 1985. Rice diseases. International Rice Research Institute.

Sitaresmi T, Yunani N, Zakki KAF, Mulsanti IW, Utomo ST, Daradjat AA. 2013. Identifikasi varietas contoh untuk karakter penciri spesifik sebagai penunjang harmonisasi pengujian BUSS padi. Jurnal Penelitian Pertanian Tanaman Pangan 32, 148–158.

Sudir S, Kadir TS. 2009. Identifikasi patotipe Xanthomonas oryzae pv. oryzae, penyebab penyakit hawar daun bakteri di sentra produksi padi di Jawa. Penelitian Pertanian Tanaman Pangan 28, 131–138.

Sudir S, Yuliani D, Wirajaswadi L. 2015. Komposisi dan Sebaran Patotipe Xanthomonas oryzae pv. oryzae, Penyakit pada Padi di Nusa Tenggara Barat. Jurnal Penelitian Pertanian Tanaman Pangan 34, 113–120.

Sun X, Cao Y, Yang Z, Xu C, Li X, Wang S, Zhang Q. 2004. Xa26, a gene conferring resistance to Xanthomonas oryzae pv. oryzae in rice, encodes an LRR receptor kinase-like protein. Plant Journal 37, 517–527.

Suparyono S, Sudir S, Suprihanto S. 2016. Pathotype profile of Xanthomonas oryzae pv. oryzae isolates from the rice ecosystem in Java. Indonesia Journal Agriculture Science. 5, 63–69.

Zhao WJ, Zhu S, Liao XL, Chen H, Tan T. 2007. Detection of Xanthomonas oryzae pv. oryzae in seeds using a specific TaqMan probe. Moleculer Biotechnology 35, 119–127.

Related Articles

Effects of three essential oils on the phytohormones production against Magnaporthe oryzae B.C. Couch, A rice blast pathogen

Ouattara Souleymane, Sama Hemayoro, Sérémé Abdoulaye, Koita Kadidia, Int. J. Agron. & Agric. Res. 27(1), 1-7, July 2025.

Boro rice cultivation practices and adaptive strategies of farmers to flash floods in Sylhet haor basin

Nurunnaher Akter, Md. Rafiqul Islam, Md. Abdul Karim, Md. Giashuddin Miah, Md. Mizanur Rahman, Int. J. Agron. & Agric. Res. 26(6), 7-18, June 2025.

Technology adoption and its impact on environmental and socioeconomic outcomes for vegetable producers in Svay Rieng Province, Cambodia

Hong Chhun, Chun Nimul, Buntong Borarin, Serey Mardy, Sao Vibol, Chan Bunyeth, Tum Saravuth, Ros Vanchey, Int. J. Agron. & Agric. Res. 26(6), 1-6, June 2025.

Effect of pigeon pea (Cajanus cajan) border crop on the control of cotton bollworms

Lovemore Mutaviri, Washington Mubvekeri, Int. J. Agron. & Agric. Res. 26(5), 122-127, May 2025.

Pruning and population density as smart solution to sustainable tomato production

Reychel I. Wamel, Artemio A. Martin Jr., Victoriano V. Casco, Lucila V. Rocha, Int. J. Agron. & Agric. Res. 26(5), 99-112, May 2025.

Enhancing growth and yield of sweet potato (Ipomoea batatas L.) through fertilizer supplementation strategies

Catherine N. Payadon, Artemio A. Martin Jr., Int. J. Agron. & Agric. Res. 26(5), 83-98, May 2025.

Propagation techniques and organic fertilizer supplementation on growth and tuber yield of yacon (Smallanthus sonchifolius)

Frimalyn P. De Vera, Artemio A. Martin Jr., Int. J. Agron. & Agric. Res. 26(5), 66-82, May 2025.