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The inheritance AT Xa27 Gene in F2 progenies of Rice crossed between ting gong and IRBB27

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Research Paper 01/10/2018
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The inheritance AT Xa27 Gene in F2 progenies of Rice crossed between ting gong and IRBB27

Farid Fitrya, Bakhtiar Basyah, Sabaruddin Zakaria
Int. J. Agron. Agri. Res.13( 4), 15-21, October 2018.
Certificate: IJAAR 2018 [Generate Certificate]
Key: Local riceMolecular analysisXa27 genXanthomonas oryzae pv. Oryzae

Abstract

Ting gong is a local rice variety that is famous for its fluffy rice texture and good adaptability. Recently this variety rarely cultivated by farmers because its deep-aged and susceptible to bacterial bligh disease. Improved performance of this variety can be done through plant breeding programs needs to be carried out. One method that can be done is crossing by utilizing the introduced variety as the source of the target gene. This study aims to analyze F2 plants that carry the Xa27 gene and evaluate their agronomic aspects. The study was conducted in a screen house, Plant Disease Laboratory and Plant Breeding Laboratory, Faculty of Agriculture, Syiah Kuala University, from July 2017 to January 2018. The seeds used were F2 progenies from a crossing between IRBB27 and local variety Ting gong. Each pot is planted with two plants and there were 12 individual plants for analysis. Resistance test for Xanthomonas oryzae pv. oryzae and molecular analysis were carried out to identify the presence of target genes (Xa27) in F2 plants. The results showed that 91.66% of the plants showed resistant symptoms (R) against Xanthomonas oryzae pv. oryzae, and 8.3% plant shows mildly susceptible to bacterial blight (MS).

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