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The morphology, anatomy and physiology characterization of mutant wheat (Triticum aestivum L.) “Alibey” in tropical lowland area

Research Paper | January 1, 2015

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Laela Sari, Agus Purwito, Didy Soepandi, Ragapadmi Purnamaningsih, Enny Sudarmonowati

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J. Bio. Env. Sci.6( 1), 395-403, January 2015


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The performance information necessary to determine mutant wheat mutant traits in the breeding program of activities to increase production. The aim of this research was to obtain variability that could be used as selection criteria and obtain an adaptive mutant wheat in area of tropical. This research was conducted in the Experimental Garden of SEAMEO-BIOTROP in Bogor with approximately 250 m. above sea level, in the period of April – November 2013. Mutants wheat “Alibey” used were 16 mutants M3 derivative resulteds from treatments using the EMS. LC50 was at 0,1% EMS for 60 minutes. Data were analyzed using a variety of methods of Augmented Design. The results showed that the mutant was affected the morphology signifantly indicated by four characters i.e. long stem panicle, grain weight/panicle, weight of 100 seeds and seed weight/plant. While there was no significant effect in the other nine characters of morphology i.e., flowering time, cooking, harvest, panicle length, plant height, number of tillers, panicle number, and leaf area. Anatomical character appearance on leaf thickness and the size of stomata showed different levels of tolerance between mutant plant (AB-0,1.60-1-7-1) and controls. As for the physiological character there were significant differences in the amount of proline and glucose levels. Proline level in control was 4.15 ug/g BB, while that in mutant “AB-0.1.60-3-16-1” was 263.47 µg/g BB, and that in “AB-0.1.60-3-3-2” was 235.90 µ/g BB. Likewise, glucose level in control was 132.88 mg/ml, while in mutant “AB-0.1.60-3-16-1” was 181.48 mg/ml, and that in “AB-0.1.60-3-3-2” was 287.41 mg/ml.


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