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The protective effect of coenzyme Q10 against methotrexate-induced hepatorenal toxicity in albino mice

Ayah H. ALhawiti, Riham A. ALBalawi, Fatemah A. Zaid, Samirah M. Alessa, Romana M. ALGhorayed, Saja A. Albalawi, Amjad Ali Khan, Munerah Al Dawsari, Mervat S. Mohamed

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Int. J. Biosci.20( 5), 120-129, May 2022

DOI: http://dx.doi.org/10.12692/ijb/20.5.120-129


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Methotrexate (MTX) is used to treat various clinical conditions, including autoimmune diseases like rheumatoid arthritis (RA), inflammatory diseases, and various cancers. However, clinical use has been restricted due to several adverse effects and toxicities. Coenzyme Q10 (CoQ10) is a safe and effective therapeutic antioxidant by being a free radical scavenger that blocks oxidative damage. CoQ10 also has anti-inflammatory effects. The study’s aim is to investigate if coenzyme Q10 (CoQ10) affects MTX-induced hepatotoxicity and nephrotoxicity. Forty albino mice were divided equally into four groups. The first group was referred to as “the control group,” and they were given a normal saline solution. The second group was dubbed the “Methotrexate group,” and they were given IP injections (10 mg/kg/day) on the fifth, sixth, and seventh days of the experiment. The third group was the CoQ10 group, which received orally administered CoQ10 (10 mg/kg/day) for seven consecutive days. The fourth group, which is the MTX+CoQ10 group, was pretreated with CoQ10 orally at a dose of 10 mg/kg/day for seven consecutive days and then received IP injections of MTX (10 mg/kg/day) for the last three days of the experiment. In mice injected with methotrexate, pretreatment with CoQ10 at a level of 10 mg/kg/day resulted in a drop in “liver function tests and renal function tests, as well as improvement in the liver and renal tissue,” according to the study. “Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as liver biomarkers, creatinine, urea, and BUN as kidney biomarkers,” as well as “histological appearance,” all improved. Coenzyme Q10 (CoQ10) protects the liver and kidneys from methotrexate-induced damage.


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The protective effect of coenzyme Q10 against methotrexate-induced hepatorenal toxicity in albino mice

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