The protective role of garlic oil against doxorubicin-induced hepatotoxicity in male mice

Paper Details

Research Paper 05/04/2023
Views (1426) Download (81)

The protective role of garlic oil against doxorubicin-induced hepatotoxicity in male mice

Abdallah M. Sliai, Gamal H. Abdel-Rahman
Int. J. Biosci.22( 4), 39-44, April 2023.
Certificate: IJB 2023 [Generate Certificate]


Doxorubicin, also known as Adriamycin, is a highly effective antineoplastic agent, but it is well known for its oxidative damage to various body organs, such as hepatotoxicity. The present study aimed to investigate the possible protective role of the natural antioxidant garlic oil on Doxorubicin-induced liver toxicity. Studies were performed on four groups of mice. Control group, garlic oil group, doxorubicin group for seven doses, and doxorubicin plus garlic oil group. Histological examination of liver sections revealed that doxorubicin caused pathological changes in liver cells as well as the reduction in the liver nuclear area and nuclear volume. Administration of garlic oil plus doxorubicin showed a reduction in liver damage induced by DOX.


Agapito MT, Antolín Y, del Brio MT, López-Burillo S, Pablos MI, Recio JM. 2001. Protective effect of melatonin against adriamycin toxicity in the rat. Journal of Pineal Research 31(1), 23-30.

Ali M, Al-Qattan KK, Al-Enezi F, Khanafer RM, Mustafa T. 2000. Effect of allicin from garlic powder onserum lipids and blood pressure in rats fed with a high cholesterol diet. Prostaglandins Leukot Essent Fatty acids 62(4), 253-259.

Anwar S, Younus H. 2017. Inhibitory effect of alliin from Allium sativum on the glycation of superoxidedismutase. International Journal. Biological Macromolecule 103, 182–193.

Asensio-Lopez MC, Sanchez-Mas J, Pascual-Figal DA, Abenza S, Perez-Marti- nez MT, Valdes M, Lax A. 2012. Involvement of ferritin heavy chain in the preventive effect of metformin against doxorubicin-induced cardiotoxicity. Free Radical Biology and Medicine.

Bilgic S, Ozgocmen M. 2019. The protective effect of misoprostol against doxorubicin induced liver injury. Biotech. Histochemietry 94, 583–591. [Google Scholar] [CrossRef] [PubMed].

Cainelli F, Vallone A. 2009. Safety and efficacy of pegylated liposomal doxorubicin in HIV-associated Kaposi’s sarcoma. Biologics 3, 385-389.

Chaudhary D, Khatiwada S, Sah S, Tamang MK, Bhattacharya S, Jha SB. 2016. “Effect of doxorubicin on histomorphology of liver of wistar albino rats,” Journal of Pharmacy and Pharmacology 4, 186-190.

El-Bakly WM, Louka ML, El-Halawany AM, Schaalan MF. 2012. 6-gingerol ameliorated doxorubicin-induced cardiotoxicity: role of nuclear factor kappa B and protein glycation. Cancer Chemotherapy and Pharmacology 70(6), 833-41.

El-Sayyad HI, Ismail MF, Shalaby FM, Abou-El-Magd RF, Gaur RL, Fernando A, Raj MH, Ouhtit A. 2009. Histopathological effects of cisplatin, doxorubicin and 5-flurouracil (5-FU) on the liver of male albino rats. International Journal of Biological Sciences 5, 466-73.

Fenwick GR, Hanley AB. 1985. The genus Allium–Part 3. Crit Rev Food Sci Nutrtion 23, 1-73.

Harunobu A, Brenda LP, Hiromichi M, Shigeo K, Yoichi I: Intake of garlic and its bioactive components. Journal of Nutrtion 131, 9555–9625.

Kalender Y. 2005. Doxorubicin hepatotoxicity and hepatic free radical metabolism in rats. The effects of vitamin E and catechin. Toxicology 209, 39–45.

Kolarovic J, Popovic M, Zlinská J, Trivic S, Vojnovic M. 2010. Antioxidant activities of celery and parsley juices in rats treated with doxorubicin. Molecules 15, 6193-6204.

Mohan M, Shashank B, Priya AV. 2014. “Protective effect of psidium guajava L. ethanolic extract on doxorubicin-induced nephrotoxicity in rats,” Indian Journal of Natural Products and Resources 5, 129-133.

Naji KM, Al-Shaibani ES, Alhadi FA, Al-Soudi SA, D’Souza MR. 2017. Hepatoprotective and antioxidant effects of single clove garlic against CCl4-induced hepatic damage in rabbits. BMC Complementary and Alternative Medicine 17, 1–12.

Osama AH, Suzan BS. 2008. “Ultrastructural Studies on the Changes Induced by Toxic Doxorubicin-Induced Testicular Cytotoxicity in Wistar Rats.” International Journal of Medicine Sciences 1, 370-400.

Prasanna, PL, Renu K, Valsala Gopalakrishnan A. 2020. New molecular and biochemical insights of doxorubicin-induced hepatotoxicity, Life Science 250, 117599.

Patel N, Joseph C, Corcoran GB, Ray SD. 2010. Silymarin modulates doxorubicin-induced oxidative stress, Bcl-xL and p53 expression while preventing apoptotic and necrotic cell death in the liver. Toxicology and Applied Pharmacology 245, 143-52.

Ray SD, Mehendale H. 2000. Apoptosis, In: Wexler, Philip (Ed.), 2nd Edition. Encyclopedia of Toxicology, Vol-I. Elsevier Science Publishing 153–178.

Rietz B, Belagyi J, Torok B, Jacob R. 1995. The radical scavenging ability of garlic examined in various models. Boll Chim Farm 13, 69-76.

Saalu LC, Enye LA, Osinubi AA. 2009. “An Assessment of the Histomorphometric Evidences of Doxorubicin-Induced Testicular Cytotoxicity in Wistar Rats.” International Journal of Medicine Sciences 1, 370-4.

Seif HS. 2016. “Physiological changes due to hepatotoxicity and the protective role of some medicinal plants,” Beni-Suef University Journal of Basic and Applied Sciences 5, 134–146.

Sheen LY, Chen HW, Kung YL, Liu CT, Lii CK. 1999. Effects of garlic oil and its organosulfur compounds on the activities of hepatic drug-metabolizing and antioxidant enzymes in rats fed high-and low-fat diets. Nutrition Cancer 35, 160-166.

Sutejo IR, Erfan E. 2017. Antioxidant and Hepatoprotective Activity of Garlic Chives (Allium tuberosum) Ethanolic Extract on Doxorubicin-Induced Liver Injured Rats. International Journal of Pharma Medicine and Biological Sciences 6, 20-23.

Vidal L, Ruiz C, Villena A, Diaz A, Perez de Vargas L. 2004. Quantitative age-related changes in dorsal lateral geniculate nucleus relay neurons of the rat. Neuroscience Research 48, 387– 396.

Wambi CO, Sanzari JK, Sayers CM. 2009. Protective effects of dietary antioxidants on proton total-body irradiation-mediated hematopoietic cell and animal survival. Radiation Research 172, 175–186.

Watanabe T, Tanaka Y. 1982. Age-related alterations in the size of human hepatocytes. A study of mononuclear and binucleate cells. Virchows Arch B Cell Pathol. Inci. Mol. Pathol 39, 9–20.