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The proteome response of barley root (Hordeum vulgare L.) to cold stress

Mahta Mohamadiaza, Mahmoud Toorchi

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J. Bio. Env. Sci.7(6), 153-161, December 2015


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Low temperature limits distribution and productivity of plants, and causes genetic, morphologic and physiologic changes. The aim of this research is to investigate the cold stress effect of 4°C on morphological traits and proteome profile of root in a cold tolerant barley cultivar (EC83-1215). For this purpose, the seeds were grown in greenhouse, and the cold stress was imposed at seedling stage. The root morphological characteristics and proteome profile were examined, after 48 hours of imposing cold stress and compared with plants grown under 25°C as control. Two-dimensional electrophoresis (2-DE) was employed for proteomic analysis and total protein was extracted using TCA-acetone method. The first dimension of the electrophoresis performed as iso-electric focusing in tube gels and the second dimension carried out using slab sodium dodecyl sulfate polyacrylamide gels. Examination of the 2-DE gels showed that among 72 repeatable protein spots, 15 proteins indicated significant changes in which, 10 and 5 protein spot were up-and down-regulated, respectively. Statistical analysis revealed that applying 4°C cold stress on plants did not change the root related traits including number of roots, root fresh weight, root volume, root surface and root diameter, statistically. Therefore, lower temperatures may be needed if we wish to see any changes in these traits.


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The proteome response of barley root (Hordeum vulgare L.) to cold stress

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