The response of spring rapeseed genotypes (Brassica napus L.) under salinity stress

Paper Details

Research Paper 01/10/2018
Views (333) Download (28)

The response of spring rapeseed genotypes (Brassica napus L.) under salinity stress

Mehdi Keshavarzian, Mahmoud Toorchi, Mohammad Reza Shakiba
J. Bio. Env. Sci.13( 4), 238-243, October 2018.
Certificate: JBES 2018 [Generate Certificate]


Abiotic stresses such as salinity, are factors that severely affects agricultural production. To evaluate the effect of salt stress on some morphological and physiological traits related to salt tolerance, 22 spring type genotypes of rapeseed (Brassica napus L.) in the vegetative growth stage an experiment was conducted as a split plot form based on Randomized Complete Blocks Design using levels of salinity: zero (control), 150 and 300mM sodium chloride with three replications were done in the research greenhouse of Tabriz university. In present study, the Na+ contents of leaves and roots and electrolyte leakage were significantly increased. Also the K+ contents of leaves and roots, K+/Na+ ratios in shoot and root, shoot height and root length were measured significantly in response to salinity stress. Significant differences between genotyps in all traits except Na+ contents of root were observed. Cluster analysis led to the separation of genotypes in to four groups. Results showed identified Safi-7, Safi-3, Hyola308, SAN-17 and SAN-2 in most traits has higher means and ranked as tolerant genotypes. The genotypes Sarigol, Safi-5, Option500, SAN-3, SAN-13, Zafar, Goliath, SAN-8 and Heros in most traits has lower means and ranked as sensitive genotypes to salinity stress. So can be used this genotypes to understanding the mechanisms involved in salt tolerance and salt tolerance genes involved in this process.


Allakhverdiev SI, Sakamoto A, Nishiyama Y, Inaba M, Murata N. 2000. Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcussp. Plant Physiology 123, 1047-1056.

Ashraf M, McNeilly T. 2004. Salinity tolerance in Brassica oilseeds. Critical Reviews in Plant Science 23, 157-174.

Athar HR, Khan A, Ashraf M. 2009. Inducing salt tolerance in wheat by exogenously applied ascorbic Acid through Different Modes. Journal of Plant Nutrition 32, 1799-1817.

Bandehhagh A, Uliaie ED, Salekdeh GH. 2013. Proteomic analysis of rapeseed (Brassica napus L.) seedling roots under salt stress. Annals of Biological Research 4, 212-221.

Bandehhagh A, Toorchi M, Mohammadi SA, Ghaparzadeh N, Hosseni Salekdeh G, Kazemnia H. 2008. Growth and osmotic adjustment of canola genotypes in response to salinity. Journal of Food, Agriculture & Environment 6, 201-208.

Dolatabadi N, Toorchi M, Shakiba MR, Kazemnia H, Komatsu S. 2012. The response and protein pattern of spring rapeseed genotypes to sodium chloride stress. African Journal of Agricultural Research 7, 755-763.

Dolatabadi N, Toorchi M. 2017. Rapeseed (Brassica napus L.) genotypes response to NaCl salinity. Journal of Biodiversity and Environmental Sciences 10, 265-270.

Farhoudi R. 2010. Effect of salt stress on antioxidant activity and seedling growth of canola (Brassica Napus L.) cultivars. International Journal of Applied Agricultural Research 5, 11-418.

Ghaedrahmati M, Mardi M, Naghavi MR, MajidiHeravan E, Nakhoda B, Azadi A. 2013. Assessment of salinity-related traits in the recombinant inbred lines population derived from the cross wheat varieties of Roshan and Sabalan. Annals of Biological Research 4, 120-126.

Iqbal M, Akhtar N, Zafar S, Ali I. 2008. Genotypic responses for yield and seed oil quality of two Brassica species under semi-arid environmental conditions. South African Journal of Botany 74, 567-571.

Khan MA, Shirazi MU, Khan MA, Mujtaba SM, Islam E, Mumtaz S, Shereen A, Ansari RU, Ashraf MY. 2009. Role of proline, K/Na ratio and chlorophyll content in salt tolerance of wheat (Triticum aestivum L.). Pakistan Journal of Botany 41, 633-638.

Kusvuran S, Yasar F, Ellialtioglu S, Abak K. 2007. Utilizing some of screening methods in order to determine of tolerance of salt stress in the melon (Cucumis melo L.). Research Journal of Agriculture and Biological Sciences 3, 40-45.

Mer RK, Prajith PK, Pandya DH, Pandey AN. 2000. Effect of salts on germination of seeds and growth of young plants of Hordeum vulgare, Triticum aestivum, Cicer arietinum and Brassica juncea. Journal of Agronomy and Crop Science 185, 209-217.

Munns R, James RA, Lauchli A. 2006. Approaches to increasing the salt tolerance of wheat and other cereals.Journal of Experimental Botany 7, 1025-1043.

Munns R, Tester M. 2008. Mechanism of salinity tolerance. Annual Review Plant Biology 59, 651-681.

Nayyar H. 2003. Accumulation of osmolytes and osmotic adjustment in water-stressed wheat (Triticum aestivum) and maize (Zea mays) as affected by calcium and its antagonists. Environmental and Experimental Botany 50, 253-264.

Sairam RK, Rao KV, Srivastava G. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science 163, 1037-1046.

Shabala S, Pottosin I. 2014. Regulation of potassium transport in plants under hostile conditions. implications for abiotic and biotic stress tolerance. Physiologia Plantarum 151, 257-279.

Van Beuningen LT, Busch RH. 1997. Genetic diversity among North American spring wheat cultivars: I., Analysis of the coefficient of parentage matrix. Crop Science 37, 570-579.

Zamani S, Nezami MT, Habibi D. 2010. quantitative and qualitative performance of four canola cultivars (Brassica nupus L.) to salinity condition. Advances in Environmental Biology 4, 422-427.