The response of yield and related traits of pinto-bean cultivars towards the use of nitrogen chemical and biological fertilizers

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Research Paper 01/10/2014
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The response of yield and related traits of pinto-bean cultivars towards the use of nitrogen chemical and biological fertilizers

Roya Jalali Condolji Mehrdad Yarnia
J. Bio. Env. Sci.5( 4), 469-478, October 2014.
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By replacing the chemical fertilizers through biological fertilizers, we can increase the yield of organic agricultural products and decrease production costs, pollution of environment and at least to reach a lasting yield in agriculture. For this purpose, effect of chemical and nitrogenous biological fertilizers has done on some traits of different cultivars of pinto-beans an experiment was conducted in the split plot factorial based on completely randomized block design with three replications during growing seasons of 2013-2014. In this experiment, The studied factors were: 6 cultivar of pinto bean such as (Talash, local Aghgouneh from Sarab, line cos16, Ghermez ghuneh From Sarab, Sadri and Khomein) as the main plot, nitrogen fertilizer in three levels as second factor: (0-50 and 100% recommended amount), biologic fertilizer in two levels: (inoculation with super plus rhizobium and without inoculation) is sub second factor. The effect of two fertilizers were significant on all the traits and the use of nitrogen fertilizer and inoculation of seeds by Rhizobium caused increasing of amount of all traits rather than traits of the controll, as the amount of the grain per pod, grain number and weight were more in all levels which were treated by biologic fertilizer rather than without biological fertilizer. In the situation of use and disuse of biologic fertilizer, the high degree in use of nitrogen fertilizer of treatment attendance up to 100% in all levels and in both situation of use and disuse of biologic fertilizer led the grain to be filled very quickly. Using the biologic fertilizer with nitrogen fertilizer caused the decrease of filling of the line cos16 and Talash. The effects of three factors were significant on the 100 kernel weight. The highest and the least 100 kernel weight in local Aqgune and line cos16 were 21.51 and 27.82 grams. In this research the seed yield which was product by biologic fertilizer with 50% chemical fertilizers more than of 100% chemical fertilizer, so far reached to needed yield, we can decrease use of chemical nitrogen fertilizer and use it as strategy for getting the sustainable agriculture.


Adholeya A, Prakash A. 2004. Effect of different organic compost/ manures on yield and yield component of bean (Phaseolus Vulgaris L). Bioresour Technol. Tanu. 92, 311–319.

Bambara S, Ndakidemi PA. 2010. Phaseolus vulgaris response to Rhizobium inoculation, lime and molybdenum in selected low pH soil in Western Cape. Journal of Agricultural Research. 5, 1804-1812.

Bhattarai N, Baral B, Shrestha G, Yami KD. 2011. Effect of mycorrhiza and rhizobium on Phaseolus vulgaris L. Scientific World. 9, 66-69.

Caranca C, Torresb MO, Baetta J. 2009. White lupine as a beneficial crop in Southern Europe. II. Nitrogen recovery in a legume-oat rotation and a continuous oat-oat. European Journal of Agronomy. 31(4), 191-197.

Chaubey PK, Singh RP. 1994. Genetic variability, correlation and path analysis ofyield components of rice. The Madras Agricultural Journal. 81, 468-470.

Dhillon G, Kler GS, Walia AS, Chahal VPS. 1980. Effect of Azotobakter chroococum and size on growth and yield of maize. Indian Journal of Agronomy. 25, 244-2490.

EL-Zeiny OAH. 2007. Effect of biofertilizers and root exudates of two weed as a source of natural growth regulators on growth and productivity of bean plants (Phaseolus vulgaris L.). Journal of Agricultural and Biological Science. 3(5), 440-446.

Eydizadeh Kh, Damghani A, Ebrahim pour F, Saahi H. 2011. The effects of amount and consumption 15 method of biological fertilizer in mix of chemical fertilizer on the function of corn. Electronic Magazine Produce of Agricultural Plants. 4(3), 25.

Franzini I, Rosario Azco N, Fernando Latanze M, Ricardo A. 2009. Interactions between Glomus species and Rhizobium strains affectthe nutritional physiology of drought-stressed legume hosts Vinicius. Journal of Plant Physiology. 167, 614-620.

Gheysari M, Mirlatifi SM, Bannayan M, Homaee M, Hoogenboom G. 2009. Interaction of water and nitrogen on maize grown for silage. Agricultural Water Management. 96,809-821.

Houshmand L. 2006. Research of effect of phosphate soil, Tiubasious Bacteria and phosphate solvers on growth and function of corn 704. Msc thesis. Eslamic Aazad University, Tabriz branch, Iran. 70 pp.

Kafi M, Kamkar B, Mahdavi Damghani AM. 2001.  Seed  biology  and  the  yield  of  grain  crops. Mashhad Ferdosi University Publication. 232 pp.

Kesel C, Hartley C. 2000. Agricultural management of grain legumes: has it led to an increase in nitrogen fixation? Field Crops Research. 65, 165-181.

Khalequzzaman KM, Hossain I. 2007. Effect of seed treatment with rhizobium strains and biofertilizers on footroot rot and yield of bushbean in fusarium soloni infested soil. Journal of Agriculture Research. 45(2), 151-160.

Lemke RL, Vanden Bygaart AJ, Campbell CA, Lafond GP, Grant B. 2009. Crop residue removal and fertilizer N: Effect on soil organic carbon in a long-term crop rotation experiment on a Udic Boroll. Agriculture Ecosystems and Environment. 135,42-51.

Mehr pouyan M, Nour mohammadi Gh, Mir Hadi MJ, Heydari Sharifabad H, Shirani rad AH. 2010. Effect of some inoculation liquid containing Legominozarum rhizobium bacteria on the absorption of food elements in 3 kinds of bean. Iranian Grain Research Journal. 1(2),1-10.

Mishra A, Prasad K, Rai G. 2010. Effect of biofertilizers inoculations on growth and yield of dwarf field pea (Pisum sativum L.) in conjunction with different doses of chemical fertilizers. Journal of Agronomy. 9, 163-168.

Mohammadi M, Majnoon Hoseini N, Esmaeili A, Dashtaki M, Mohammad Ali pour H. 2011. The research of the effects of coexisting of Rhizobium and nitrogen fertilizer on amount of bean. (Phaseolous vulgaris L.). Iranian Agricultural Plants Science Journal. 3, 535-543.

Namvar A, Khandan T, Shojaei M. 2012. Effects of bio and chemical nitrogen fertilizer on grain and oil yield of sunflower under different rates of plant density. Scholars Research. 3(2), 1125-1131.

Norouzi V, Dideban B, Ebadi pour A. 2010. The effect of different levels of nitrogen and inoculation of seed with bacteria on the function of Azad kind of peas in tropical part of Gachsoran. The 5th National Conference of New Opinions in Agriculture of Esfahan. 17-18 Feb. pp: 134.

Radwan SMA, Awad NM. 2002. Effect of soil Amendent with various organic wastes with multi-biofertilizer on yield of peanut plants in sandy soil. Journal of Agriculture Sciences of Mansoura Univ. 27(5), 3129-3138.

Rodriguez-Navarro DN, Buendia AM, Camacho M, Lucas MM. 2000. Characterization of Rhizobium spp bean isolates from southwest Spain. Soil Biology and Biochemistry. 32, 1601-1613.

Safapour M, Ardakani MR, Rejali F, KHaghani Sh, Teymouri M. 2010. The effect of dual inoculation of Microriza and Rhizobium on function of 3 kinds of red bean (Phaseolus vulagris L.) The New Achivments. 1,21-35.

Saharan BS, Nehra V. 2011. Plant growth promoting Rhizobacteria: A critical review. Journal of Life Sciences and Medicine Research. LSMR-21.

Sharief AE, EL_Kalla SE, El_Kassaby AT, Ghonema MH, Abdo GMQ. 2006. Effect of Bio_chemical fertilization and times of nutrient foliar application on growth, yield and yield components of rice. Journal of Agronomy. 5(2), 212-219.

Sharifi Z, Haghnia Gh. 2006. Effect of Nitroxin biological fertilizer on the function of wheat from Sabalan. The second Ecological Agriculture National Conferences. 15-16 Oct. Gorgan-Iran. 1795-1806.

Singh S, Kappor K. 1998. Effects of inoculation of phosphate solubilizing microorganism and arbusccular mycorriza fungus on munybeen grown under natural soil condition. Mycorriza. 7(5), 249-253.

Upadhyay RG, Sharma S, Daramwal NS. 1999. Effect of Rhizobium and graded levels of phosphorus on the growth and yield of summer green gram (Phaseolus radiates). Legume Research. 22(4), 277-279.

Yadegari M. 2014. Inoculation of Bean (Phaseolus vulgaris) seeds with Rhizobium phaseoli and plant growth promoting Rhizobacteria. Advances in Environmental Biology. 8(2), 419-424.

Yazdani M, Bahmanyar MA, Pirdashti H, Esmaili MA. 2009. Effects of phosphate solubilizing microorganisms (PSM) and plant growth promoting rhizobacteria (PGPR) on yield and yield components of Corn (Zea mays L.). World Academy of Science, Engineering and Technology. 37, 90-92.