Welcome to International Network for Natural Sciences | INNSpub

The role of exogenous salicylic acid (SA) on phytohormonal changes and drought tolerance in common bean (Phaseolus vulgaris L.)

Research Paper | December 1, 2012

| Download 5

O. Sadeghipour, P. Aghaei

Key Words:

J. Bio. Env. Sci.2( 12), 8-15, December 2012


JBES 2012 [Generate Certificate]


Salicylic acid (SA) is an endogenous plant growth regulator of phenolic nature, which participates in the regulation of physiological processes in plants and also plays an important role in provoking plant resistance to various abiotic stresses. An experiment was therefore, conducted to evaluation the effect of exogenous application of SA on the changes of phytohormones and biomass of common bean under water stress conditions during 2011 in Iran. Seeds were soaked at various concentrations of SA (0, 0.25, 0.5, 0.75 and 1 mM) for 6 h. Then plants were subjected to normal and drought conditions. Drought decreased significantly endogenous auxin (IAA), gibberellic acid (GA3) and cytokinin (CK) levels while increased abscisic acid (ABA) content. Water stress also reduced biomass production. SA-pretreatment (especially 0.5 mM) enhanced IAA, GA3 and CK levels whereas decreased ABA content under both conditions and so improved biomass production. Results suggest that SA can alleviate the adverse effects of drought in common bean via changes in levels of endogenous plant hormones.


Copyright © 2012
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

The role of exogenous salicylic acid (SA) on phytohormonal changes and drought tolerance in common bean (Phaseolus vulgaris L.)

Abass SM, Mohamed HI. 2011. Alleviation of adverse effects of drought stress on common bean (Phaseolus vulgaris L.) by exogenous application of hydrogen peroxide. Bangladesh Journal of Botany 41 (1), 75-83.

Abdalla MM. 2011. Beneficial effects of diatomite on the growth, the biochemical contents and polymorphic DNA in Lupinus albus plants grown under water stress. Agriculture and Biology Journal of North America 2 (2), 207-220.

Abdalla MM, El-Khoshiban NH. 2007. The influence of water stress on growth, relative water content, photosynthetic pigments, some metabolic and hormonal contents of two Triticium aestivum cultivars. Journal of Applied Sciences Research 3 (12), 2062-2074.

Bandurska H, Stroinski A. 2005. The effect of salicylic acid on barley response to water deficit. Acta Physiologiae Plantarum 27 (3B), 379-386.

Bano A, Yasmeen S. 2010. Role of phytohormones under induced drought stress in wheat. Pakistan Journal of Botany 42 (4), 2579-2587.

Beebe S, Rao IM, Cajiao C, Grajales M. 2008. Selection for drought resistance in common bean also improves yield in phosphorus limited and favorable environments. Crop Science 48, 582-592.

Davenport TL, Morgan PW, Jordan WR. 1980. Reduction of auxin transport capacity with age and internal water deficit in cotton petioles. Plant Physiology 65, 1023-1025.

El-Mergawi R, Abdel-Wahed M. 2007. Diversity in salicylic acid effects on growth criteria and different indole acetic acid forms among faba bean and maize. International Plant Growth Substances Association.19 Annual meeting, Puerto Vallarta, Mexico, July 21-25, 2007.

Farooq M, Basra SMA, Wahid A, Ahmad N, Saleem BA. 2009. Improving the drought tolerance in rice (Oryza sativa L.) by exogenous application of salicylic acid. Journal of Agronomy and Crop Science 195 (4), 237-246.

Gharib  FA,  Hegazi  AZ.  2010. Salicylic acid ameliorates germination, seedling growth, phytohormone and enzymes activity in bean (Phaseolus vulgaris L.) under cold stress. Journal of American Science 6 (10), 675-683.

Graham HP, Ranalli P. 1997. Common bean (Phaseolus vulgaris L.). Field Crops Research 53, 131-146.

Haji MAA, Bukhsh A, Malik AU, Ishaque M, Sadiq SH. 2009. Performance of sunflower in response to exogenously applied salicylic acid under varying irrigation regimes. The Journal of Animal & Plant Sciences 19 (3), 130-134.

Hao JH, Wang XL, Dong CJ, Zhang ZG, Shang QM. 2011. Salicylic acid Induces stomatal closure by modulating endogenous hormone levels in cucumber cotyledons. Russian Journal of Plant Physiology 58 (5), 906-913.

Hayat Q, Hayat S, Irfan M, Ahmad A. 2010. Effect of exogenous salicylic acid under changing environment: A review. Environmental and Experimental Botany 68, 14-25.

Idrees M, Naeem M, Aftab T, Masroor M, Khan A, Moinuddin. 2011. Salicylic acid mitigates salinity stress by improving antioxidant defense system and enhances vincristine and vinblastine alkaloids production in periwinkle [Catharanthus roseus (L.) G. Don]. Acta Physiologiae Plantarum 33 (3), 987-999.

Kaur S, Gupta AK, Kaur N. 1998. Gibberelic acid and kinetin partially reverse the effect of water stress on germination and seedling growth. Plant Growth Regulation 25, 29-33.

Khan W, Prithviraj B, Smith DL. 2003. Photosynthetic responses of corn and soybean to foliar application of salicylates. Journal of Plant Physiology 160, 485-492.

Metwally A, Finkemeier I, Georgi M, Dietz K. 2003. Salicylic acid alleviates the cadmium toxicity in barley seedlings. Plant Physiology 132, 272-281.

Morgan PW. 1990. Effects of abiotic stresses on plant hormone systems. In: Stress Responses in Plants: Adaptation and Acclimation Mechanisms. Alscher, R. G. and J. R. Cumming (Eds). Wiley-Liss, New York, pp. 113-146.

Noushina I, Rahat Nazar M, Iqbal R, Khan AM, Nafees AK. 2011. Role of gibberellins in regulation of source-sink relations under optimal and limiting environmental conditions. Current Science 100, 998-1006.

Parent B, Hachez C, Redondo E, Simonneau T, Chaumont F, Tardieu F. 2009. Drought and abscisic acid effects on aquaporin content translate into changes in hydraulic conductivity and leaf growth rate: A trans-scale approach. Plant Physiology 149, 2000-2012.

Poljakoff-Mayber A, Lerner HR. 1994. Plants in saline environments. In: Handbook of Plant and Crop Stresses. Pessarkali, M. (Ed.). Marcel Dekker Inc., New York, pp. 65-96.

Pospisilova J, Vagner M, Malbeck J, Travnickova A, Batkova P. 2005. Interactions between abscisic acid and cytokinins during water stress and subsequent rehydration. Biologia Plantarum 49 (4), 533-540.

Pustovoitova TN, Zhdanova NE, Zholkevich VN. 2004. Changes in the levels of IAA and ABA in cucumber leaves under progressive soil drought. Russian Journal 0f Plant Physiology 51, 513-517.

Rai VK, Sharma SS, Sharma S. 1986. Reversal of ABA-induced stomatal closure by phenolic compounds. Journal of Experimental Botany 37, 129-134.

Sadeghipour O, Aghaei P. 2012a. Biochemical changes of common bean (Phaseolus vulgaris L.) to pretreatment with salicylic acid (SA) under water stress conditions. International Journal of Biosciences 2 (8), 14-22.

Sadeghipour O, Aghaei P. 2012b. Response of common bean (Phaseolus vulgaris L.) to exogenous application of salicylic acid (SA) under water stress conditions. Advances in Environmental Biology 6 (3), 1160-1168.

Sakhabutdinova AR, Fatkhutdinova DR, Bezrukova MV, Shakirova FM. 2003. Salicylic acid prevents the damaging action of stress factors on wheat plants. Bulgarian Journal of Plant Physiology, special issue 314-319.

Sakurai N, Akiyama M, Kuraishi S. 1985. Role of abscisic acid and indoleacetic acid in the stunted growth of water-stressed, etiolated squash hypocotyls. Plant and Cell Physiology 26, 15-24.

Saruhan N, Saglam A, Kadioglu A. 2011. Salicylic acid pretreatment induces drought tolerance and delays leaf rolling by inducing antioxidant systems in maize genotypes. Acta Physiologiae Plantarum 34 (1), 97-106.

Shakirova FM, Sakhabutdinova AR, Bezrukova MV, Fatkhutdinova RA, Fatkhutdinova  DR. 2003.  Changes  in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science 164 (3), 317-322.

Shi JP, Li GM, Chi SM. 1994. The effect of calcium on endogenous hormones of maize seedlings under water stress. Journal of Hebei Agricultural University 17, 48-51.

Shindy WW, Smith OE. 1975. Identification of plant hormones from cotton ovules. Plant Physiology 55, 550-554.

Steel RGD, Torrie JH. 1980. Principles and procedures of statistics. A biometrical approach. 2nd Ed. McGraw-Hill Book Co. Inc., New York, 633 p.

Subbaro GV, Johansen C, Slinkard AE, Nageswara Rao RC, Saxena NP, Channah YS. 1995. Strategies for improving drought resistance in grain legumes. Critical Reviews in Plant Sciences 14 (6), 469-523.

Wang  C,  Yang  A,  Yin  H,  Zhang  J.  2008. Influence of water stress on endogenous hormone contents and cell damage of maize seedlings. Journal of Integrative Plant Biology 50 (4), 427-434.

Waseem M, Athar UH, Ashraf M. 2006. Effect of salicylic acid applied through rooting medium on drought tolerance of wheat. Pakistan Journal of Botany 38 (4), 1127-1136.

Xie Z, Jiang D, Cao W, Dai T, Jing Q. 2003. Relationships of endogenous plant hormones to accumulation of grain protein and starch in winter wheat under different post-anthesis soil water statuses. Plant Growth Regulation 41, 117-127.

Xie Z, Jiang D, Dai T, Jing Q, Cao W. 2004. Effect of exogenous ABA and cytokinin on leaf photosynthesis and grain protein accumulation in wheat ears cultured In vitro. Plant Growth Regulation 44, 25-32.

Yang JC, Zhang JH, Wang ZQ, Zhu QS, Wang W. 2001. Hormonal changes in the grains of rice subjected to water stress during grain filling. Plant Physiology 127, 315-323.

Zhang J, Smith DL, Liu W, Chen X, Yang W. 2011. Effects of shade and drought stress on soybean hormones and yield of main-stem and branch. African Journal of Biotechnology 10 (65), 14392-14398.