The study of genetic diversity in different plant populations of Artemisia Annua L. native to northern Iran by molecular marker AFLP
Paper Details
The study of genetic diversity in different plant populations of Artemisia Annua L. native to northern Iran by molecular marker AFLP
Abstract
Artemisia annua L. is an annual, medicinal and aromatics plant that is widespread in Asia, Europe and North America and has been wide distribution in northern areas of Iran. In this study, AFLP markers were used to investigate the genetic diversity of 15 populations of A. annua. 86 scorable bands were produced by using four primer combinations )E/M(, that 58 of them) were polymorphic. The highest number of polymorphic bands (24 bands) using primer combination EcoR11-Mse20 and the lowest number of polymorphic (6 bands) using primer combinations EcoR2-Mse35 were produced. Data matrix using similarity coefficient was converted to similarity matrix (distance matrix), and then using the algorithm (UPGMA) was drawn relevant dendrogram. Dendrogram obtained from UPGMA put 15 populations in four groups. The obtained cluster of cases was consistent with the geographic diversity in some cases. Then correlation coefficient based on the Mantel test was obtained 7.0, indicating good agreement drawn dendrogram with the similarity matrix. The results of principal component analysis and almost coincided with the results of cluster analysis. The results showed that the use of AFLP method to assess molecular diversity in these masses is appropriate.
Asghari A, Razban Haghighi A. 1999. Classification of Artemisia species for resistance to salinity. Fifth Congress of agronomy and plant breeding in Iran, Karaj, 259.
Bagchi GD, Haider F, Dwivedi PD, Singh A, Naqvi AA. 2003. Essential oil constituents of Artemisia annua during different growth periods at monsoon conditions of subtropical north Indian plains. Journal of Essential Oil Research 15(4), 248-250. http://dx.doi.org/10.1080/10412905.2003.9712131
Bertea CM, Voster A, Verstappen FWA, Maffei M, Beekwilder J, Harro JB. 2006. Isopernoid biosynthesis in Artemisia annua: cloning and hetrologous expression of a germacrene A synthase from a glandular trichome cDNA library. Biochemistry and Biophysics 448 (1-2), 3-12. PMID: 16579958.
Bhakuni RS, Jain DC, Sharma RP, Kumar S. 2001. Secondary metabolites of Artemisia annua and their biological activity. Current Science 80(1), 35-48.
Bovard R, Rezazadeh ShA, Naghavi MR, Omidi M, Torabi S, Parvane S, Hariri Akbari F, Taghizad Farid R. 2014. Variation in the Essential oil of Artemisia annua L. Apical Shoots at Different Developmental Stages. Iranian journal of horticultural sciences 45(3), 319-324.
Cafferata LFR, Gatti WO, Mijailosky S. 2010. Secondary gaseous metabolites analyses of wild Artemisia annua L. Molecular Medicinal Chemistry 21, 48-52.
Charles DJ, Cebert E, Simon JE. 1991. Characterization of the essential oil of Artemisia annua L. Journal of Essential oil Research 3(1), 33-39. http://dx.doi.org/10.1080/10412905.1991.9697903
Ellis RP, Forster BP, Gordon DC, Handley LL, Keith RP, Lawrence P, Meyer R, Powell W, Robinson D, Scrimgeour CM, Young G. Thomas WTB. 2002. Phenotype/genotype associations for yield and salt tolerance in a barley mapping population segregating for two dwarfing genes. Journal of Experimental Botany 53 (371), 1163-1176. PMID: 11971927.
Etminan A, Omidi M, Majidi Hervan E, Naghavi MR, Reza zadeh Sh, Pirseyedi M. 2012. The study of genetic diversity in some Iranian accessions of Hyoscyamus sp. using amplified fragment length polymorphism (AFLP) and retrotransposon/AFLP markers. 2012. African Journal of Biotechnology 11(43), 10070-10078. http://dx.doi.org/10.5897/AJB11.2106
Gupta SK, Singh P, Bajpai P, Ram G, Singh D, Gupta MM, Jain DC, Khanuja SP, Kumar S. 2002. Morphogenetic variation artemisinin and volatile oil in Artemisia annua. Industrial crops and products 16(3), 217-224. http://dx.doi.org/10.1016/S0926-6690(02)00049-3
Hariri Akbari F, Omidi M, Etminan AR, Shafiee M, Parvaneh S, Bord R. 2012. The presentation of easy method for extraction of DNA from medicinal plant. Research – Scientific Quarterly Plant and Ecosystem 7(29-2), 11-21.
Hariri Akbari F, Omidi M, Torabi S, Khoshkharam M, Bovard R, Shafiee M, Safakish kashani MB, Saeed P, Behjat Sasan B. 2014. Journal of Biodiversity and Environmental Sciences (JBES) 4(6), 141-152. ISSN: 2220-6663 (Print) 2222-3045 (Online). http://www.innspub.net
Hashemi H, Safarnejad A, Bagheri A. 2009. Investigation of genetic variation among Iran’s Persian Zira (Bunium persicum Boiss) landraces using RAPD marker. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research 16 (2), 238-246. ISSN: 1735-0891
Hoareau L, DaSilva EJ. 1999. Medicinal plants: a re-emerging health aid. EJB Electronic Journal of Biotechnology 2(2). http://www.ejbiotechnology.info/content/ http://dx.doi.org/10.2225/vol2-issue2-fulltext-2
Kant T, Parmar AK. 2014. Efficient somatic embryogenesis and molecular marker based analysis as effective tools for conservation of red-listed plant Commiphora wightii. Journal of BioScience and Biotechnology 3(2), 169-182. ISSN: 1314-6238 (Print); 1314-6246 (Online).
Kermani M, Marashi H, Nasiri MR, Safarnejad A, Shahriari F. 2006. Study of genetic variation of Iranian cumin lines (cuminum cyminum) using AFLP markers, Iranian Food Science and Technology Research Journal 20, 305-312.
Khunani Z. 2008. Assessment of Genetic diversity in the Samples of Ferula gummosa from Iran using AFLP markers. Master Thesis. University of Tehran, Karaj.
Lari Yazdi H, Khavari Nejad R, Rustaian A. 2001. Identification of essential oil composition of Artemisia annua collected from North regions of Iran. Medicinal Plants Journal 1, 39-46.
Majruhi, AA. 2008. Study of variation in quantity and quality of the essential oil of Zhumeria majdae Rech. F at different growth stages. Medicinal Plant Journal 29, 107-113.
Mueller MS, Runyambo N, Wagner I, Borrmann S, Dietz K, Heide L. 2004. Randomized controlled trial of a traditional preparation of Artemisia annua L. (Annual Wormwood) in the treatment of malaria. Transactions of the Royal Society of Tropical Medicine and Hygience 98(5), 318-321. PMID: 15109558. http://dx.doi.org/.1016/j.trstmh.2003.09.001
Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breed 2(3), 225-238. http://dx.doi.org/10.1007/BF00564200.
Rezaei L, Qaderi A, Naghavi MR, Ebrahimi MA, Riazi AS, Mehrafarin A, Naghdi Badi H. 2012. Assessment of Genetic Diversity in the Populations of Hypericum perforatum L. Using AFLP Markers. Journal of Medicinal plants 11(44), 62-70.
Santos CAF, Simon PW. 2002. Some AFLP amplicons are highly conserved DNA sequences mapping to the some linkage groups in tow F2 populations of carrot. Genet. Mol. Biol 25(2), 195-201. http://dx.doi.org/10.1590/S141547572002000200013
Sarwat M, Nabi G, Das S, Srivastava PS. 2012. Molecular markers in medicinal plant biotechnology: past and present, Critical Reviews in Biotechnology 32(1), 74-92. http://dx.doi.org/10.3109/07388551.2011.551872
Schippmann U, Leaman DJ, Cunningham AB. 2002. Impact of cultivation and gathering of medicinal plants on biodiversity: global trends and issues. In: Biodiversity and the Ecosystem Approach in Agriculture, Forestry and Fisheries. FAO, 1–21.
Sharafi A, Hashemi Sehi H, Kazemi Tabar K. 2006. A review of tissue culture, regeneration and genetic transformation of Artemisia annua. Medicinal Plants Journal 20, 1-10.
Siahsar B, Rahimi M, Tavassoli A, AbdolShakoor Raissi. 2011. Application of Biotechnology in Production of Medicinal Plants. American-Eurasian J. Agric. and Environ. Sci., 11(3), 439-444. ISSN 1818-6769© IDOSI Publications
Tasheva K, Kosturkova G. 2012. The Role of Biotechnology for Conservation and Biologically Active Substances Production of Rhodiola rosea: Endangered Medicinal Species. The Scientific World Journal 2012, 1-13. http://dx.doi.org/10.1100/2012/274942
Tzenkova R, Kamenarska Z, Draganov A, Atanassov A. 2010. Composition of Artemisia annua L. Essential oil obtained from species growing wild in Bulgaria. Biotechnol & Biotechnol 24(2), 1833-1835. http://dx.doi.org/10.2478/v10133-010-0030-6
Verdianrizi MR. 2008. Variation in the essential oil composition of Artemisia annua L. Of different growth stages cultivated in Iran. African Journal of Plant Science 2(2), 016-018. ISSN: 1996-0824.
Wright CW. 2002. Artemisia. Taylor and Fransis Inc., New York.
Yu HH, Yang ZL, Sun B, Liu RN. 2011. Genetic diversity and relationship of endangered plant Magnolia officinalis (Magnoliaceae) assessed with ISSR polymorphisms. Biochem. syst. Ecol 39(2), 71-78. http://dx.doi.org/10.1016/j.bse.2010.12.003
Bovard Reza, Naghavi Mohammad Reza, Omidi Mansoor, Torabi Sepide, Rezazadeh Shamsali, Hariri Akbari Farhad, Shafie Alavijeh Mehdi, Behjat Sasan Baharak, Parvaneh Saeed, Safakish Kashani Mohammad Bagher (2016), The study of genetic diversity in different plant populations of Artemisia Annua L. native to northern Iran by molecular marker AFLP; JBES, V9, N2, August, P51-59
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