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The study of genetic relationships between in landrace chickpea collected from north-west of Iran using SCoT molecule marker

Fatemeh Pakseresht, Reza Talebi, Ezat karami, Hooman Shirvani

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J. Bio. Env. Sci.4(3), 12-19, March 2014

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Abstract

This study was carried out using 10 SCoT primers, to genetic relationships evaluation for 38 accessions of 4 population’s chickpea of the north-west of Iran. In addition, 95 bands were scored using these primers, that polymorphism were showed for 65 bands. Primer SCoT12 with 14 bands had the highest and primer SCoT35 with 5 bands had the lowest number of bands. The lowest percent of polymorphism belonged to SCoT11 (33%) and the primer of SCoT13 had the highest percent of polymorphism (100%). AMOVA revealed that 12% of the total variance was due to differences between populations and 88% was due to differences within populations. Sanandaj population with the Shannon’s information index (I) and Nei’s gene diversity (He) had the highest variety between reviewed population, and Kurdistan population had the lowest variety. In addition the population of Kurdistan had the highest distance with Qorve and the population of Kurdistan had the highest similarity with Sanandaj. These results were confirmed by cluster analysis and principal coordinate analysis of populations. Cluster analysis and Scatter plot based on first and second axis from principal coordinate analysis for accessions, showed that genetic variation did not agreement with the geographical distribution.

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The study of genetic relationships between in landrace chickpea collected from north-west of Iran using SCoT molecule marker

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