Thermotherapy of citrus budwood to control Candidatus Liberibacter asiaticus transmission for huanglongbing management

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Research Paper 01/08/2020
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Thermotherapy of citrus budwood to control Candidatus Liberibacter asiaticus transmission for huanglongbing management

Muhammad Sarwar Yaqub, Iqrar Ahmad Khan, Fatima Ismail, Rozina Aslam
Int. J. Biosci. 17(2), 72-82, August 2020.
Copyright Statement: Copyright 2020; The Author(s).
License: CC BY-NC 4.0

Abstract

Citrus greening or huanglongbing (HLB) is the most destructive disease of the citrus. Citrus trees infected by phloem resident Candidatus liberibacter asiaticus, the causal organism of huanglongbing either by its natural host Diaphorina citri kuwayama (Hemiptera: Psyllidae) or during budding/grafting process are demanding to cure in the field, greenhouse and nurseries by cultural practices. Climate has a serious effect on the evolution of liberibacter species associated with citrus huanglongbing.  Thermotherapy of citrus budwood was conducted in the current study for cleaning the huanglongbing positive budwood to control Candidatus Liberibacter asiaticus transmission for HLB management. Budwood was obtained from PCR tested HLB positive sweet orange trees and subjected to temperature treatment ranging from 56 °C to 68 °C. Temperature treated budwood was then grafted on citrus jambhiri lush rootstocks. Real-time PCR assays revealed that thermotherapy at 68 °C is effective to inhibit the transmission of Candidatus Liberibacter asiaticus from budwood to rootstock.

Aslam R, Khan IA, Rahman KU, Asghar M. 2017a. Detection of ‘Candidatus Liberibacter asiaticus’ the causal organism of huanglongbing, in mandarin group of citrus. International Journal of Agriculture and Biology 19, 255‒258. http://dx.doi.org/10. 17957/IJAB/15.0271.

Aslam R, Khan IA, Rahman KU, Asghar M, Yaqub MS. 2017b. Expression profiling of huanglongbing disease in kinnow (Citrus reticulata) and succari (Citrus sinensis) leaves. International Journal of Agriculture and Biology 19, 1187‒1192. http://dx.doi.org/10.17957/IJAB/15.0409.

Baldwin E, Plotto A, Manthey J, McCollum G, Bai J, Irey M, Cameron R. 2010. Effect of liberibacter infection (huanglongbing disease) of citrus on orange fruit physiology and fruit/fruit juice quality: Chemical and physical analyses. Journal of Agriculture and Food Chemistry 58, 1247-1262.

Bove JM. 2006. Huanglongbing: a destructive, newly emerging, century old disease of citrus. Journal of Plant Pathology 88, 7-37.

Doud MS, Hoffman MT, Zhang M, Stover E, Hall D, Zhang S, Duan Y. 2012. Thermal treatments eliminate or suppress the bacterial pathogen in huanglongbing-affected citrus. Poster Session: Biological Control-IPM-Sanitation, ID: 124-P. APS Annual meeting 4-8 August.

Doud MS, Wang Y, Hoffman MT, Latza CL, Luo W, Armstrong CM, Gottwald T, Dai L, Luo F, Duan Y. 2017. Solar thermotherapy reduces the titer of Candidatus Liberibacter asiaticus and enhances canopy growth by altering gene expression profiles in HLB affected citrus plants. Horticulture Research 4, 1-9. http://dx.doi.org/10.1038/hortres.2017.54

Garnier M, jagoueix S, Cronje PR, Roux GF, Bove JM. 2000. Genomic characterization of a Liberibacter present in an ornamental rutaceous tree, Calodendrum capense, in the Western Cape Province of South Africa. Proposal of Candidatus Liberibacter africanus sub sp. capensis. International Journal of Systemic and Evolutionary Microbiology 50, 2119-2125.

Graça JV, Korsten L. 2004. Citrus huanglongbing: review, present status and future strategies, p 229-245. In: SAMH. Naqvi (ed.). Diseases of fruit and vegetables. 1, Kluwer Academic, the Netherlands.

Halbert SE, Manjunath KL. 2004. Asian citrus psyllids (Sternorrhyncha: psyllidae) and greening disease of citrus: A literature review and assessment of risk in Florida. Florida Entomology 87, 330-353.

Hocquellet A, Toorawa P, Bove JM, Garnier M. 1999. Detection and identification of two Candidatus liberobacter species associated citrus huanglongbing by PCR amplification of ribosomal protein genes of the beta operon. Molecular and Cellular Probes 13, 373-379.

Hoffman MT, Doud MS, Williams L, Zhang MQ, Ding F,Stover E, Hall D, Zhang S,  Jones L, Gooch M, Fleites L, Dixon W, Gabriel D, Duan YP. 2013. Heat treatment eliminates ‘Candidatus Liberibacter asiaticus’ from infected citrus trees under controlled conditions. Phytopathology 103, 15-22.

Huang CH. 1978. Effect of hot air treatment on likubin, Tristeza virus and exocortis viroid diseases of citrus. Journal of Agriculture Research in China 27, 193-197.

Hussain MA, Nath D. 1927. The citrus psylla (Diaphorina citri Kuwayama) [Psyllidae:Homoptera]. Memories of the Department of Agriculture in India, Entomological Series 10, 1-27.

Jagoueix S, Bove JM, Garnier M. 1994. The Phloem-limited bacterium of greening is a member of the alpha subdivision of the proteobacteria. International Journal of Systematic Bacteriology 44, 379-386.

Jagoueix S, Bove JM, Garnier M. 1996. PCR detection of two candidatus Liberibacter species associated with greening disease of citrus. Molecular and Cellular Probes 10, 43-50.

Lin KH. 1964. A preliminary study on the resistance of yellow shoot virus and citrus budwood to heat. Acta Phytopathologica Sinica 7, 61-63.

Lin KH. 1956. Observation of yellow shoot on citrus. Etiological studies of yellow shoot on citrus. Acta Phytopathologica Sinica 2, 1-42.

Li W, Hartung JH, Levy L. 2006. Quantitative real time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbing. Journal of Microbiological Methods 66,104-115.

Nariani TK, Ghosh SK, Kumar D, Raychaudhuri SP, Wishwanath SM. 1975. Detection and possibilities of therapeutic control of the greening disease of citrus caused by mycoplasma. Proceeding of Indian Natural Science Academic Series B 41, 334-339.

Teixeira DC, Danet JL, Eveillard S, Martins EC, Junior WCJ, Yamamoto PT,   Lopes SA, Bassanezi RB, Ayers AJ, Sailard C, Bove JM. 2005. Citrus huanglongbing in Sao Paulo State, Brazil: PCR detection of the Candidatus Liberibacter species associated with the disease. Molecular and Cellular Probes 19, 173-179.

Westbrook CJ, Hall DG, Stover E, Duan YP. 2011. Colonization of Citrus and Citrus related Germplasm by Diaphorina citri (Hemiptera: Psyllidae). Hortscience 46, 997–1005.

Yaqub MS, Khan IA, Usman M, Rana IA. 2017. Molecular detection of Candidatus Liberibacter asiaticus, the causal organism of huanglongbing (citrus greening) in Faisalabad, Pakistan for huanglongbing management. Pakistan Journal of Agricultural Sciences 54, 21-26. http://dx.doi.org/10.21162/PAKJAS/17.4455.

Yaqub MS, Khan IA, Aslam R. 2019. Citrus budwood treatment with antibiotic and temperature to control Candidatus Liberibacter asiaticus transmission for huanglongbing management. International Journal of Biosciences 15, 150-159. http://dx.doi.org/10.12692/ijb/15.2.150-159.

Zhang M, Guo Y,Powell CA,Doud MS, Yang C, Duan Y. 2014. Effective antibiotics against Candidatus Liberibacter asiaticus in HLB-affected citrus plants identified via the graft-based evaluation. PLoS One 9(11), e111032. http://dx.doi.org/10.1371.

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