Thermotherapy of citrus budwood to control Candidatus Liberibacter asiaticus transmission for huanglongbing management

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Research Paper 01/08/2020
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Thermotherapy of citrus budwood to control Candidatus Liberibacter asiaticus transmission for huanglongbing management

Muhammad Sarwar Yaqub, Iqrar Ahmad Khan, Fatima Ismail, Rozina Aslam
Int. J. Biosci.17( 2), 72-82, August 2020.
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Citrus greening or huanglongbing (HLB) is the most destructive disease of the citrus. Citrus trees infected by phloem resident Candidatus liberibacter asiaticus, the causal organism of huanglongbing either by its natural host Diaphorina citri kuwayama (Hemiptera: Psyllidae) or during budding/grafting process are demanding to cure in the field, greenhouse and nurseries by cultural practices. Climate has a serious effect on the evolution of liberibacter species associated with citrus huanglongbing.  Thermotherapy of citrus budwood was conducted in the current study for cleaning the huanglongbing positive budwood to control Candidatus Liberibacter asiaticus transmission for HLB management. Budwood was obtained from PCR tested HLB positive sweet orange trees and subjected to temperature treatment ranging from 56 °C to 68 °C. Temperature treated budwood was then grafted on citrus jambhiri lush rootstocks. Real-time PCR assays revealed that thermotherapy at 68 °C is effective to inhibit the transmission of Candidatus Liberibacter asiaticus from budwood to rootstock.


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