Welcome to International Network for Natural Sciences | INNSpub

Thin layer chromatographic profiling and phytochemical screening of six medicinal plants in Bangladesh

Research Paper | July 1, 2017

| Download 60

Tanvir Ahamed, S. K. Mizanur Rahman, Abdullah Mohammad Shohael

Key Words:

Int. J. Biosci.11( 1), 131-140, July 2017

DOI: http://dx.doi.org/10.12692/ijb/11.1.131-140


IJB 2017 [Generate Certificate]


Medicinal plants contain different bioactive compounds which have great importance to the health of individuals and communities. These compounds produce definite physiological action on the human body. The present study was carried out to verify the thin layer chromatographic (TLC) profiling and phytochemical screening of water and ethanol extracts of six different plant parts in Bangladesh. These are Young Mango fruit, Radhuni leaves, Dill leaves, Thankuni leaves, Mature Chalta fruit and Olive fruit. Phytochemical screening was performed by various qualitative methods and TLC profiling was carried out using a various solvent system of varying polarity. Qualitative phytochemical determination by chemical test reflects the presence of alkaloid, steroid, saponin, tannin, leucoanthocyanin, coumarin, flavonoid, reducing sugar, glycoside and phenol in different plants. TLC profiling of these plants were carried out using different solvents of ethanol extracts and it showed different Rvalue. The results obtained in the present investigation indicated that the six plants are rich source of various secondary metabolites. It is also observed that alkaloid and coumarin are common in most of the selected plants. This can be further investigated for the isolation and identification of the active biochemical compound of medicinal utilities in a cost-effective way.


Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Thin layer chromatographic profiling and phytochemical screening of six medicinal plants in Bangladesh

Akindele AJ, Adeyemi OO. 2007.  Anti inflammatory activity of the aqueous leaf extract of Byrsocarpus coccineus. Fitoterapia 78, 25-28.

Beckman CH. 2000. Phenolic-storing cells: keys to programmed cell death and periderm formation in wilt disease resistance and in general defense responses in plants. Physiological and Molecular Plant Pathology 57, 101-110. https://doi.org/10.1006/pmpp.2000.0287

Biradar SR, Rachetti BD. 2013. Extraction of some secondary metabolites & thin layer chromatography from different parts of Centella asiatica L. (URB). American Journal of Life Sciences 1, 243-247. https://doi.org/10.11648/j.ajls.20130106.11

Bregum F. 2010. The present status of Medicinal Plants in Bangladesh. Iranian Journal.

Bregum F. 2010. The present status of Medicinal Plants in Bangladesh. Iranian Journal of Pharmaceutical Research 3, 34-34.

Callan NW, Johnson DL, Westcott  MP, Welty LE. 2007. Herb and oil composition of dill (Anethum graveolens L.): Effects of crop maturity and plant density. Industrial Crops and products 25, 282-287. https://doi.org/10.1016/j.indcrop.2006.12.007

Chung KT, Wong TY, Wei CI, Huang YW, Lin Y. 1998. Tannins and human health: a review. Critical reviews in food science and nutrition 38, 421-464. http://dx.doi.org/10.1080/10408699891274273

Factor R. 1991. Thin layer chromatography. Wiley Blackwell. 159–173.

Gibbs RD. 1974. Chemotaxonomy of Flowering plant Morinda citrifolia L. Queen’s University Press. Montreal and London.

Gujjeti RP, Mamidala E. 2013. Phytochemical screening and thin layer chromatographic studies of Aerva lanata root extract. International Journal of Innovative Research in Science, Engineering and Technology 2, 5725-5730.

Hesse M. 2002. Alkaloids: nature’s curse or blessing? John Wiley & Sons.

Inamdar PK, Yeole RD, Ghogare, AB, De Souza NJ. 1996. Determination of biologically active constituents in Centella asiatica. Journal of Chromatography 742, 127-130. https://doi.org/10.1016/0021-9673(96)00237-3

Khurram M, Khan MA, Hameed A, Abbas N, Qayum A, Inayat H. 2009. Antibacterial activities of Dodonaea viscosa using contact bio-autography technique. Molecules 14, 1332-1341. https://doi.org/10.3390/molecules14031332

Kren, Martínková L. 2001. Glycosides in medicine: “The role of glycosidic residue in biological activity”. Current Medicinal Chemistry 8, 1303-1328. https://doi.org/10.2174/0929867013372193

Kumar  S, Kumar V, Prakash O. 2013.Enzymes inhibition and antidiabetic effect of isolated constituents from Dillenia indica. Bio-Med research international 10, 101-106.http://dx.doi.org/10.1155/2013/382063

Kumar A, Ilavarasan R, Jayachandran T, Decaraman M, Aravindhan P, Padmanabhan N, Krishnan MRV. 2009. Phytochemicals investigation on a tropical plant, Syzygium cumini from Kattuppalayam, Erode district, Tamil Nadu, South India. Pakistan Journal of Nutrition 8, 83-85.

Lee ER, Kang GH, Cho SG. 2007. Effect of flavonoids on human health: old subjects but new challenges. Recent Patents on Biotechnology 1, 139-150. https://doi.org/10.2174/187220807780809445

Li H, Miyahara T, Tezuka Y, Namba T, Nemoto N, Tonami S, Seto H, Tada T,Kadota S. 1998. The effect of Kampo formulae on bone resorption in vitro and in vivo. I. Active constituents of Tsu-kan-gan. Biological and Pharmaceutical Bulletin 21, 132-226. http://doi.org/10.1248/bpb.21.1322

Lokanathan Y, Omar N, Ahmad Puzi NN, Saim A, HjIdrus R. 2016. Recent Updates in Neuroprotective and Neuroregenerative Potential of Centella asiatica. Malaysian Journal of Medical Sciences 23, 4-14.

Nandagoapalan V, Doss A, Marimuthu C. 2016. Phytochemical analysis of some traditional medicinal plants. Bioscience Discovery 7, 17-20.

Nstro A, Germano MP, D’angelo V, Marino A, Cannatelli MA. 2000. Extraction methods and bio-autography for evaluation of medicinal plant antimicrobial activity. Letters in Applied Microbiology 30, 379-384. http://doi.org/10.1046/j.1472-765x.2000.00731.x

Khodadadi I, Tavilani H, Goodarzi MT. 2016.Effect of dill tablet (Anethum graveolens L) on antioxidant status and biochemical factors on carbon tetrachloride-induced liver damage on rat. International Journal of Applied and Basic Medical Research 6, 111. http://doi.org/10.4103/2229-516X.179019

Pandey P, Mehta R, Upadhyay R. 2013. Physico-chemical and preliminary.

Pandey P, Mehta R, Upadhyay R. 2013. Physico-chemical and preliminary phytochemical screening of Psoralea corylifolia. Archives of Applied Science Research 5, 261-265.

Patil AM, Nasreen S. 2016.Screening for Secondary Metabolites of Some Important Medicinal Plants. PARIPEX-Indian Journal of Research 4, 10-15.

Peerakam N, Punjaisee S, Buamongkol S, Sirisa-ard P, Julsrigival J, Chansakaow S. 2014.Chemical compositions, antioxidant and antibacterial activitie s of essential oils from Anethum graveolens L. and Trachyspermum roxburghianum (DC.) Craib grown in Thailand. Journal of Natural Sciences Research 4.

Pereira AP, Ferreira IC, Marcelino F, Valentão P, Andrade PB, Seabra R, Estevinho L, Bento A, Pereira JA. 2007. Phenolic compounds and antimicrobial activity of olive (Olea europaea L. Cv. Cobrançosa) leaves. Molecules 12, 1153-162. http://doi.org/10.3390/12051153

Pichersky E, Gang DR. 2000. Genetics and biochemistry of secondary metabolites in plants: an evolutionary perspective. Trends in plant science 54, 39-445.

Qian Y, Tang X, Guan T, Li Y, Sun, H. 2016. Neuroprotection by Combined Administration with Maslinic Acid, a Natural Product from Olea europaea, and MK-801 in the Cerebral Ischemia Model. Molecules 21, 1093. http://doi.org/10.3390/molecules21081093

Rizk AM. 1982. Constituents of plants growing in Quatar. 1. A chemical survey of sixty plants. Fitoterapia 53, 35-44.

Santhi R, Lakshmi G, Priyadharshini AM,  Anandaraj L. 2011. Phytochemical screening of Nerium oleander leaves and Momordica charantia leaves. International research journal of pharmacy 2, 131-135.

Satyawati GV, Gupta AK. 1987. Medicinal plants of India: Indian Council of Medical Research, New Delhi.

Shah KA, Patel MB, Patel RJ, Parmar PK. 2010. Mangifera indica (mango). Pharmacognosy reviews 4, 42. http://doi.org/10.4103/0973-7847.65325

Sharma HK, Chhangte L, Dolui AK. 2001. Traditional medicinal plants in Mizoram,  India. Fitoterapia 72, 146-161. https://doi.org/10.1016/S0367-326X(00)00278-1

Shi J, Arunasalam K, Yeung D, Kakuda Y, Mittal G, Jiang Y. 2004.Saponins from edible legumes: chemistry, processing, and health benefits. Journal of Medicinal Food 7, 67-78.  https://doi.org/10.1089/109662004322984734

Stahl E. 2013. Thin-layer Chromatography: A Laboratory Handbook. Springer Science & Business Media.

Stoddard JM, Nguyen L, Mata-Chavez H, Nguyen K. 2007. TLC plates as a convenient platform for solvent-free reactions. Chemical Communications 12, 1240-1241.

Subramanian D. 1986. Cytotaxonomical studies in South Indian Apiaceae. Cytologia 51, 479-488. http://doi.org/10.1508/cytologia.51.479

Tseng A. 1991. Chemoprevention of tumors in MTV-H ras transgenic mice with coumarins. In Proceedings Amstardam Association Cancer Research 32, 380.

Ugochukwu SC, Uche A, Ifeanyi O. 2013. Preliminary phytochemical screening of different solvent extracts of stem bark and roots of Dennettia  tripetala G. Baker. Asian Journal of Plant Science and Research 3, 10-13.

Verpoorte R. 1999. Chemodiversity and the biological role of secondary metabolites, some thoughts for selecting plant material for drug development. In Bioassay Methods in Natural Product Research and Drug Development, 11-23. http://doi.org/10.1007/978-94-011-4810-8-2