Total aflatoxin levels on imported Maize through Gazetted and Un-gazetted points of Entries in Kenya

Paper Details

Research Paper 15/06/2023
Views (765)
current_issue_feature_image
publication_file

Total aflatoxin levels on imported Maize through Gazetted and Un-gazetted points of Entries in Kenya

Joseph Oduor Odongo, Paul O. Angi’enda, Bramwel Wanjala, Catherine Taracha, David M. Onyango
J. Biodiv. & Environ. Sci. 22(6), 167-176, June 2023.
Copyright Statement: Copyright 2023; The Author(s).
License: CC BY-NC 4.0

Abstract

Maize is a crucial staple crop that serves as both food and feed in Kenya. However, its widespread cultivation in tropical and subtropical climates often results in contamination with Aspergillus flavus during transportation and storage. The main objective of this study was to assess moisture content and level of aflatoxin contamination present in imported maize from Uganda, the major supplier to Kenya. In situ measurements were taken to determine moisture content. Aflatoxin levels were analyzed using the total aflatoxin (B1, B2, G1, and G2) ELISA method. The study utilized IBM SPSS version 20.0 software to conduct data analysis. The results revealed a statistically significant positive correlation between maize moisture content and aflatoxin (AF) levels at the three points of entry (POEs) in Malaba, Sioport and Busia. This correlation, although considered low with a coefficient of 0.122, indicated that an increase in maize moisture content was associated with a limited increase in AF levels. Out of the 600 representative samples collected from the POEs, the majority exhibited AF levels below the threshold of 10 parts per billion (ppb), with an average level of 2.68ppb. However, 25 samples exceeded the threshold, with the highest level recorded at 27.97ppb. The moisture content of the samples ranged from 9.05% to 14.2%, averaging at 11.6%.These findings indicate that most imported maize samples complied with the regulatory threshold for AF levels, while only a small portion exceeded it. The estimated prevalence rate of AF contamination in the study was 4.17%, significantly lower than the estimated rate in Kenya. These results highlight the commendable efforts of the regulatory agencies at the border in ensuring compliance with the regulatory threshold for imported maize. Nonetheless, there remains a need to implement effective inspection, monitoring, testing, and surveillance measures to further enhance food safety.

Abbas HK, Masiello M, Shier WT, Horn BW, Sobolev VS, Cartwright RD. 2016. Effect of kernel maturity and environmental factors on aflatoxin and fumonisin contamination in commercial corn (Zea mays) hybrids in Arkansas. Journal of Agricultural and Food Chemistry 64(47), 9075-9083.

Amakhobe T. 2017. Mycoflora of Layer Feed in Nairobi County and Their Toxigenic Potential [Master’s thesis, University of Nairobi]. Retrieved from http://erepository.uonbi.ac.ke/bitstream /handle/11295/155798/ Amakhobe%20T_ Mycoflo ra% 20of%20Layer%20Feed%20in%20Nairobi% 20County%20and%20Their%20Toxigenic%20Potential.pdf?isAllowed=y&sequence=1

Bukowski J. 2019. “Aflatoxins: Occurrence and toxicology,” Journal of Food Protection 82(1), 1-18.

Centers for Disease Control and Prevention. 2022. Aflatoxin. Retrieved from https://www.cdc.gov /fungal/diseases/aflatoxin/index.html

Chen X. 2018. Aflatoxin contamination in food crops: Aflatoxin biosynthesis, effects of abiotic stressors, and detection methods. Food Control 89, 54-64.

Egbuta MA. 2018. Mycotoxin contamination of cereal crops and staples: A review on occurrence, toxicity, and regulatory practices in Nigeria. Toxins 10(9), 363.

FAO/WHO. 2002. Aflatoxins in food. Joint FAO/WHO Food Standards Programme, Codex Alimentarius Commission.

Food and Agriculture Organization of the United Nations. 2018. Aflatoxins. Retrieved from http://www.fao.org/aflatoxins/en

Grace D, Lindahl J, Mutua F, Kang’ethe E, Jones K. 2015. Aflatoxin Contamination of Maize in Kenya: Observations from a Pilot Study. Available at: https://cgspace.cgiar.org/bitstream/handle/10568/66498/Aflatoxin%20Contamination%20of%20Maize%20in%20Kenya%20-%20Observations%20from% 20a%20 Pilot%20Study.

Kyallo M, Monda E, Imungi J, Kilonzo-Nthenge A, Grace D. 2015. Aflatoxin contamination in maize and groundnut in Western Kenya. Journal of Applied Biosciences 89, 8297-8305.

Ministry of Health. 2018. National Guidelines for the Prevention and Management of Aflatoxins Contamination in Kenya. Government of Kenya.

Mutungi C, Affognon H, Abong’o B, Harvey J. 2019. Fighting aflatoxin in Africa: progress, challenges, and opportunities. In Mycotoxins and Food Safety pp. 1-28. Springer, Cham.

Njoroge SMC, Matumba L, Kanenga K, Siambi M, Wanyama R, Tongoona P, Okori P. 2010. Post-harvest interventions for control of aflatoxin in maize and groundnuts of smallholder farmers in Africa. African Journal of Food, Agriculture, Nutrition and Development 10(2), 425-441.

Ojiambo PS, Lusty C, Mwamburi LA. 2005. Relationship between maize grain moisture content and aflatoxin contamination in Eastern Kenya. African Journal of Food, Agriculture, Nutrition and Development 5(1), 1-15.

Omara E. 2021. Mycotoxins in food: A review of their occurrence, health effects, and control measures, Food Science and Technology 13, 1-14.

Oyelade OJ, Oyelade OJ, Adejumo IO, Adebo OA. 2021. Assessment of aflatoxin levels and moisture content of maize grain in Nigeria. Journal of Food Quality 2021, 1-8.

Paster N, Fuentes S, Arroyo-Manzanares N. 2021. Analysis of moisture content and total aflatoxin ELISA test in imported maize to ensure regulatory compliance. Journal of Food Protection 84(8), 1394-1399. doi: 10.4315/JFP-21-082.

Peraica M, Radic B, Lucic A, Pavlovic M. 1999. Toxic effects of mycotoxins in humans. Bulletin of the World Health Organization 77(9), 754-766.

Atehnkeng J, Ojiambo PS, Ikotun T, Sikora RA, Cotty PJ, Bandyopadhyay R. 2008. Evaluation of atoxigenic isolates of Aspergillus flavus as potential biocontrol agents for aflatoxin in maize. Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment 25(10), 1264-1271.

Shirima CP, Kimanya ME, Routledge MN, Srey C, Kinabo JL, Humpf HU, Wild CP. 2015. A prospective study of growth and biomarkers of exposure to aflatoxin and fumonisin during early childhood in Tanzania. Environmental health perspectives 123(2), 173-178.

Shuaib FM. 2020. A review on the synergistic effect of fumonisins with aflatoxins in animals. Toxins 12(11), 698.

Smith T. 2018. “Aflatoxins and liver cancer,” Cancer Research 78(2), 321-328.

Torres AM, Barros GG, Chulze SN, Ramirez ML. 2011. Impact of environmental factors on growth and production of aflatoxins by Aspergillus section Flavi strains isolated from peanut crops in Argentina. World Mycotoxin Journal 4(3), 283-290.

Wang L. 2016. Risk factors for hepatocellular carcinoma, World Journal of Gastroenterology 22(16), 4187-4197.

Wild CP, Gong YY. 2010. Mycotoxins and human disease: a largely ignored global health issue. Carcinogenesis, 31(1), 71-82. doi:10.1093/carcin

Wild CP. 2018. “Aflatoxins: An overview of the most toxic mycotoxins,” Toxins, vol. 10(3), pp. 97.

Wild CP, Turner PC, Smith TM. 2004. Mycotoxins and their toxic effects. Journal of Applied Microbiology 96(4), 572-585.

Woldegiorgis AZ, Zenebe G, Habtewold T. 2019. Correlation of maize kernel moisture content with aflatoxin contamination level. Journal of Food Quality 2019, 1-7.

Wu F. 2010. Mycotoxin reduction in Bt corn: potential economic, health, and regulatory impacts. Transgenic Research 19(4), 607-611. DOI: 10.1007/s11248-010-9399-5

Wu F, Bhatnagar D, Bui-Klimke T, Carbone I, Hellmich R, Munkvold G, Payne G. 2019. Climate change impacts on mycotoxin risks in US maize. World Mycotoxin Journal 12(1), 69-78.

Related Articles

Design and development of a sustainable chocolate de-bubbling machine to reduce food waste and support biodiversity-friendly cacao processing

John Adrian B. Bangoy, Michelle P. Soriano, J. Biodiv. & Environ. Sci. 27(4), 41-47, October 2025.

Ecological restoration outcomes in Rwanda’s Rugezi wetland: Biodiversity indices and food web recovery

Concorde Kubwimana, Jean Claude Shimirwa, Pancras Ndokoye, J. Biodiv. & Environ. Sci. 27(4), 32-40, October 2025.

Noise pollution in the urban environment and its impact on human health: A review

Israa Radhi Khudhair, Bushra Hameed Rasheed, Rana Ihssan Hamad, J. Biodiv. & Environ. Sci. 27(4), 28-31, October 2025.

Prevalence of Anaplasma marginale and Ehrlichia ruminantium in wild grasscutter’ specific ticks in southern Côte d’Ivoire

Zahouli Faustin Zouh Bi, Alassane Toure, Yatanan Casimir Ble, Yahaya Karamoko, J. Biodiv. & Environ. Sci. 27(4), 21-27, October 2025.

Impact of social media campaigns on farmers awareness of environmental conservation practices

Preeti Raina, Rahul Kumar Darji, Rahul Mittal, J. Biodiv. & Environ. Sci. 27(4), 1-8, October 2025.

Phytochemical analysis and antioxidant activity of ethanolic leaves extract of Psidium guajava

G. Saranya, K. Durgadevi, V. Ramamurthy, J. Biodiv. & Environ. Sci. 27(3), 57-63, September 2025.

Physicochemical and phytochemical analysis of Glycyrrhiza glabra root extract

J. Ramalakshmi, P. Vinodhiniand, V. Ramamurthy, J. Biodiv. & Environ. Sci. 27(3), 50-56, September 2025.