Toxic and teratogenic effects of mycelia and fruiting body extracts of Lentinus strigosus (BIL 1324) in zebra fish (Danio rerio) embryo

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Research Paper 01/11/2018
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Toxic and teratogenic effects of mycelia and fruiting body extracts of Lentinus strigosus (BIL 1324) in zebra fish (Danio rerio) embryo

Rich Milton R. Dulay, Danielle G. Pamiloza, Rolen L. Ramirez
Int. J. Biosci.13( 5), 205-211, November 2018.
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We reported in the previous works the successful rescue, domestication, optimization, enriched cultivation, compositions, and functional activities of Lentinus strigosus, a naturally occurring, wood-rotting edible mushroom. Herein, the embryo-toxic and teratogenic effects of water extracts of mycelia and fruiting body of this mushroom in zebra fish (Danio rerio) embryos were investigated. The toxic effects of both extracts were dependent on concentration and time of exposure. Embryos at 1000 µg/ml (after 24 hrs) and 100 µg/ml (after 48 hrs) and lower concentrations of mycelial extract survived while 100% mortality was recorded to those exposed at 10000 µg/ml (after 24 hrs) and 1000 µg/ml (after 48 hrs) concentration of fruiting body extract. Both extracts significantly reduced the hatchability and heartbeat rate of zebra fish. Delayed development was the sole teratogenic effect and this was obvious to embryos exposed at 100 µg/ml of both mycelia and fruiting body extracts. Therefore, L. strigosus holds promising potential as source of teratogens, which are also known as anticancer agents.


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