Toxic effects of sub-lethal dose of algal toxin (Microcystin-LR) on male laboratory mice Mus muscullus L.

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Research Paper 01/11/2017
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Toxic effects of sub-lethal dose of algal toxin (Microcystin-LR) on male laboratory mice Mus muscullus L.

Emad yousif Awad AL-Sultan, Selma Saeed Abbas
Int. J. Biosci.11( 5), 192-203, November 2017.
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The present study was aimed to investigate the toxic effects (physiological and histological effects) of sub-lethal dose of microcystin-LR (0.125 µg/kg/day as i.p. injection) on male mice for long time of exposure15and 30 days .Sixty four intact adult male mice (Laboratory generations) were used in current study. Animals were divided into eight groups each one contain eight male mice. Each group treated with 0.1 ml of toxins. Results showed significant decrease in sperm account reach 37and 25× 104 for the two periods respectively as compared with control group. While testosterone levels increased significantly p≤0.05 after 30 days reach 0.14 ng/ml compared with control group. However non-significant increase in follicle Stimulating (FSH) and luteinizing Hormone (LH) Hormone for two exposure periods. A significant reduction in the diameter of somniferous tubules and number of spermatogonium for two exposure periods reach 45.57, 37.50 µm and 28.37, 28 µm unit respectively compared with control groups. Whereas, the diameter of germ cells was not affected significantly. Liver enzymes such as (i.e. Aspartate transaminase (AST), Alanine transaminase (ALT) were increase significantly for two periods of exposure reach 161.66 , 179 IU/L and 158.33, 180 33 IU/L respectively, While alkaline phosphatase (ALP)activity do not showed any significant increasing . Finding also appeared many histopathological changes in testis combined with exposure periods increasing and compared with control group represented by congestion, thickness of capsule wall, necrosis, excessive vacillation and closer of some somniferous tubules. In conclusion, the sub lethal dose of MC-LR may be led highly impairment in male mice testes; function and histology, sex hormones and liver enzymes when exposing for long period reach to 30 days that may be led to Infertility.


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