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Research Paper | September 1, 2015

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Toxicity activity of Azadirachta indica and Murraya koenigii against Aedes albopictus larvae in laboratory conditions

Muthulinggam Nishan, Partiban Subramanian

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J. Bio. Env. Sci.7(3), 27-35, September 2015

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Abstract

Dengue serves as major threat to human beings and has caused an increase in the rate of mortality every year without considering age, gender or ethnicity. Thus, an alternative by using botanical extracts against the larvae of this mosquito was carried out. Azadirachta indica and Murraya koenigii were used to evaluate the larvacidal activity against 3rd instar of Aedes albopictus larvae. The bioassay was carried out by using the methanolic extract of the A. indica and M. koenigii and the combined activity was done with the ratio 1:1 of the plant extracts. The result showed that at 3.75 mg/ml A.indica exhibits highest larvacidal activity by providing 96.30% of mortality. Furthermore, M. koenigii showed the percentage of mortality of 83.70% at 3.75mg/ml. In the basis of median lethal concentration (LC50), it was found that the LC50 for A. indica was 1.45 mg/ml which is more susceptible than M. koenigii with 2.01 mg/ml as a LC50 value. The combination test showed that interaction between the 2 plants extract as an additivity effect. In nut shell, A. indica and M. koenigii was found to exhibit strong larvacidal activity. Thus, these plants have high potential to be developed as a biopesticide to control the emergence of A. albopictus larvae.

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Toxicity activity of Azadirachta indica and Murraya koenigii against Aedes albopictus larvae in laboratory conditions

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