Toxicological evaluation of Commiphora swynnertonii stem bark exudates on mice and rats

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Research Paper 01/07/2017
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Toxicological evaluation of Commiphora swynnertonii stem bark exudates on mice and rats

Sylvester G. Temba, Paul Erasto, Disela E. Swai, Musa Chacha
J. Bio. Env. Sci.11( 1), 321-329, July 2017.
Certificate: JBES 2017 [Generate Certificate]

Abstract

The aim of this study was to evaluate the safety of Commiphora swynnertonii stem bark exudates by determining its acute and sub-acute toxicity in mice and rats. The acute toxicity of exudates was administered to mice by oral administration of a single dose of tested concentrations and observed for 14 days. The doses were 500, 1000 and 3000mg/Kg body weight. The sub-acute toxicity test of the exudate was conducted on rats through a daily oral administration of various doses for 28 days. The doses were 250, 500 and 1000mg/Kg body weight. The physiological, behavioral and body weight change as well as relative weight of internal organs were subsequently recorded. Hematological, biochemical and histopathological parameters for rats used in the sub-acute toxicity assay were also measured at day 28. Results showed that, administration of up to 3000 mg/Kg body weight the C. swynnertonii exudates in mice did not result into any observable toxicity or mortality within the period of 14 days. Furthermore, the sub-acute toxicity test revealed that the exudates did not induce mortality neither behavioral nor physiological changes after 28 days. It was further observed that, the rat’s body and relative internal organs weight as well as hematological, biochemical and histopathological parameters did not change as results of receive the doses for the whole period of the study. Therefore this study has revealed that C. swynnertonii stem bark exudates is safe for oral administration at low and moderate doses.

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Adeneye A, Ajagbonna O, Adeleke T, Bello S. 2006. Preliminary toxicity and phytochemical studies of the stem bark aqueous extract of Musanga cecropioides in rats. Journal of Ethnopharmacology, 105, 374-379.

Alam MB, Hossain M. sarowar, Chowdhury NS, Mazumder MEH, Haque ME, Islam A. 2011. In vitro and in vivo antioxidant and toxicity evaluation of different fractions of oxalis corniculata Linn. Journal of Pharmacology and Toxicology 6(4), 337-348.

Aleman CL, Noa M, Mas R, Rodeiro I, Mesa R, Menendez R, Gamez R. 2015. Reference data for the principal physiological indicators in three species of laboratory animals. Laboratory Animals, 2000 379-385.

Badarunisha, Rao YR, Devi PU, Rafiqkhan M. 2014. Acute and sub acute Toxicological evaluation of Ethanolic extract of hypericum Hookerisnum. World Journal of Pharmacy and Pharmaceutical Sciences 3(6), 909-920.

Bakari G, Max R, Mdegela H, Phiri E., Mtambo M. 2012. Effect of crude extracts from Commiphora swynnertonii (Burtt) against selected microbes of animal health importance. Journal of Medicinal Plants Research 6(9), 1795-1799.

Baranitharan M, Dhanasekaran S, Gokulakrishnan J, Babu SM. 2016. Larvicidal and oviposition deterrent activity of Nagapattinam medicinal plants against the dengue fever mosquito , Aedes aegypti. International Journal of Mosquito Research, 3(3), 29-34.

Bulus T, Atawodi SE, Mamman M. 2011. Acute toxicity effect of the aqueous extract of Terminalia avicennioides on white albino rats. World, Science Vol, Journal, 6(2), 2-5.

Deepa J, Gokulakrishnan J, Baranitharan M, Dhanasekaran S. 2015. Larvicidal activity of Indian medicinal plants on the Dengue fever mosquito, Aedes aegypti linnaeus. International Journal of Pure and Applied Zoology 3(2), 130-136.

Diallo A, Eklu-Gadegkeku K, Agbonon A, Aklikokou A, Creppy E, Gbeassor M. 2010. Acute and Subacute (28-Day) Oral Toxicity Studies of Hydroalcoholic Extract of Lannea kerstingii Engl. and K. Krause (Anacardiaceae) Stem Bark. Journal of Pharmacology and Toxicology 5(7), 343-349.

Gautam MK, Goel RK. 2014. Toxicological Study of Ocimum sanctum Linn Leaves: Hematological, Biochemical, and Histopathological Studies. Journal of Toxicology.

Kalala W, Magadula J, Mdegela H. 2014. Evaluating Acaricidal Activity of Commiphora swynertonii ( Burrt .) bark Exudate against common Ticks in Tanzania. International Jornal of Herbal Medicine 2(4), 19-25.

Kripa KG, Chamundeeswari D, Thanka J. 2011. Acute and sub-acute toxicity Evaluation of ethanolic extract of Leucas aspera (Lamiaceae) in experimental rats. International Journal of Drug Development & Research 3(3), 339-347.

Mdegela RH, Bakari GG, Simon S, Mosha TEC. 2017. Effects of Commiphora swynnertonii on weight and plasma cholesterol levels in Rattus rattus. Journal of Medicinal Plants Research 11(11), 226-231.

Mkangara M, Erasto P, Chacha M. 2014. Acaricidal Activity of Commiphora Swynnertonii (Burtt) Stem Bark Extracts against adult Rhipicephalus Appendiculatus Newman and Amblyomma Variegatum. American Journal of Research Communication 2(9), 82-92.

Mukinda JT, Syce JA. 2007. Acute and chronic toxicity of the aqueous extract of Artemisia afra in rodents. Journal of Ethnopharmacology 112(1), 138-144.

Musa A. 2008. Antioxidant and Antibacrerial activity of commiphora kerstingii Engl.Stem back Extract. Research Journal of Phytochemistry 2(2), 106-111.

Nagagi YP, Silayo RS, Kweka EJ. 2016. Trypanocidal activity of ethanolic extracts of Commiphora swynnertonii Burtt on Trypanosoma congolense. BMC Complementary and Alternative Medicine 16(1).

OECD. 2001. Acute Oral Toxicity – Acute Toxic Class Method. Oecd Guideline for Testing of Chemicals 1-14.

Ouedraogo GG, Ouedraogo M, Lamien-sanou A, Lompo M, Olga M, Guissou PI. 2013. Acute and Subchronic Toxicity Studies of Roots Barks Extracts of Calotropis procera (Ait.) R . Br Used in the Treatment of Sickle Cell Disease in Burkina Faso. British Journal of Pharmacology and Toxicology 4(5), 194-200.

Paraskeva MP, van Vuuren SF, van Zyl RL, Davids H, Viljoen AM. 2008. The in vitro biological activity of selected South African Commiphora species. Journal of Ethnopharmacology 119(3), 673-679.

Rahman MM, Garvey M, Piddock LJ, Simon G. 2008. Antibacterial terpenes from the oleo-resin of Commiphora molmol (Engl.). Phytotherapy Research 22, 1356-1360.

Reddy YRR, Lokanatha O, Ratnam K, Reddy CS, Raju IN, Damodar RC. 2013. Acute and Sub Acute Toxicity of Moringa Oleifera Stem Bark Extract in Swiss Albino Mice. International Journal of Life Sciences Biotechnology and Pharma Research, 2(4), 73-82.

Sini S, Sinha B, Rajasekaran A. 2010. Acute toxicity studies of aqueous leaf extract of Capparis grandiflora wall Ex Hook.f and Thomson. Journal of Chemical and Pharmaceutical Research 2(6), 112-117.

Van Wyk BE, Wink M. 2004. Medicinal Plants of the World. An Illustrated Scientific Guide to Important Medicinal Plants and Their Uses. Journal of Ethnobiology 355-356.

Willbrord MK, Joseph JM, Robinson HM. 2014. Ethnobotanical use of Commiphora swynertonii Burrt. amongst Dorobo people in Tanzania. Journal of Medicinal Plants Research 8(23), 820-828.