Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | March 1, 2015

| Download 2

Toxicological impact of a mimetic estrogen specie: mancozeb on a tadpoles of the green frog (Rana saharica)

Benosmane Sana, Djebar Mohammed Reda, Berrebah Houria, Alayat Amel, Benamara Marwa, Zouaghi Mohamed Fateh, Amamra Rima

Key Words:

J. Bio. Env. Sci.6(3), 307-314, March 2015


JBES 2015 [Generate Certificate]


The presence in the environment of chemicals with hormone-mimetic properties generates concerns about their effects on aquatic organisms. Due to their estrogenic activity, they are part of the chemical compounds whose concentration level in the environment isn’t monitored. The estrogenic substances can be of natural origin, but also of synthetic origin such as certain pesticides. In order to prevent the risks associated with better use and exposure to xeno-estrogens, we have investigated the effects of estrogen mimetic (MOS) fungal origin (Mancozeb) on the morphology of bio-indicators organisms of pollution: the tadpoles of the green frog (Rana saharica.) Treated with different concentrations 0.25, 0.5, 0.75 and 1 mg/l over a period of 5 weeks. This xeno-estrogen induced disturbances of growth and a condition index tadpoles showing a net delay of the breeding and a affection of sexual maturity. We also observe that the exposure of tadpoles populations to different concentrations of the fungicide disrupts their respiratory metabolism in dose-dependent manner. The inhibition of respiration resulted an increase in free radical production, which is confirmed by the test of quantization of reactives metabolites of oxygen (d-ROMs) which highlights a significant production of reactive oxygen species (ROS).


Copyright © 2015
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Toxicological impact of a mimetic estrogen specie: mancozeb on a tadpoles of the green frog (Rana saharica)

Alberti A, Bolognini L, Macciantelli D, Caratelli M. 2000. The radical cation of N,N-diethyl-para-phenylendiamine: a possible indicator of oxidative stress in biological samples. Research on Chemical Intermediates 26, 253–267.

Blaustein A, Johnson P. 2003. The complexity of deformed amphibians. Frontiers in ecology and the environment 1, 87-94.

Botosoa JA. 2010. Purification and partial chemical and biological characterization of the active extracts from leaves Pechia madagascariensis (APOCYNACEAE). PhD thesis, University of Antananarivo, Madagascar.

Bouaricha H, Berrebbah H, Grara N, Djebar MR. 2012. Response of paramecium sp. with respect to an insecticide (Proclaim): growth, content of MDA, AChE activity and respiratory metabolism. Journal of Applied Sciences Research 8, 4172-4180.

Boulenger GA. 1913. On a Collection of Batrachians and Reptiles made by Dr. H.G.F. Spurrell, F.Z.S., in: the Choco, Colombia. In: Proceeding of the Zoological Society of London 83, 1019-1038.

Brahamia K, Semouk A. 2010. Tourist activity in a fragile snack space of El Kala National Park. El-Tawasso 26.

Bridges CM, Semlitsch RD. 2000. Variation in pesticide tolerance of tadpoles among and within species of Ranidae and patterns of amphibian decline. Conservation Biology 14, 1490-1499.

Brion F, Tyler CR, Palazzi X, Garric J, Laillet B, Porcher JM, Flammarion P. 2004. Impacts of 17beta-estradiol, including environmentally relevant concentrations, on reproduction after exposure during embryo-larval-, juvenile- and adult-life stages in zebrafish (Danio rerio). Aquatic Toxicology 68, 193-217.

Castelle AJ, Johnson AW, Conolly C. 1994. Wetlands and Stream Buffer Size Requirements. A Review. Journal of Environmental Quality 23, 878-882.

Celi P, Sullivan M, Evans D. 2010. The stability of the reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests on stored horse blood. The Veterinary Journal 183, 217–218.

Chagra A, Rouabhi R, Berrebbah H, Djebar MR. 2007. The toxicity study of a systemic fungicide: artea 330 EC on the physiology and the respiratory metabolism of the tadpole (Rana saharica). Communications of Agricultural and Applied Biological Sciences 72, 191-5.

Denver RJ. 1997. Proximate mechanisms of phenotypic plasticity in amphibian metamorphosis. American Zoologist 37, 172–184.

Dunson WA, Connell J. 1982. Specific-inhibition of hatching in amphibian embryos by low pH. Journal of Herpetology 16, 314-316.

Flouriot G, Pakdel F, Ducouret B, Ledrean Y, Valotaire T. 1997. Differential regulation of two genes implicated in fish reproduction: vitellogenin and estrogen receptor genes. Molecular Reproduction and Development 48, 317-23.

Gomez E. 2003. Emerging contaminants Liaison with estrogen receptor: examples for different classes of substances. HSM Hydrosciences, Montpellier 1, France.

Gosner KL. 1960. A Simplified Table for Staging Anuran Embryos and Larvae. Herpetologica 16, 183-190.

Joly P. 2000. Biological invasions: state of the art and perspectives. Journal of Ecology (Earth and Life) 7, 21-35.

Jung RE, Jagoe CH. 1995. Effects of low pH and aluminum on body size, swimming performance and suceptibility to predation of green tree frog (Hyla cinerea) todpoles. Canadian Journal of zoology 73, 2171- 2183.

Kiesecker JM, Blaustein AR, Belden LK. 2001. Complex  causes of amphibian population declines. Nature 410, 681-684.

Laskowski R, Hopkin SP. 1996. Effect of Zn, Pb and Cd on fitness in snails (Helix aspersa). Ecotoxicology Environmental Safety 34, 59-69.

Le cren ED. 1951. The length-weight relationship and seasonal cycle in gonad weight I and condition in the perch (Perca fluviatilis). Journal of Animal Ecology 20, 201-19.

Le guevel R, Pakdel F. 2001. Assessment of oestrogenic potency of chemicals used as growth promoter by in-vitro methods. Human Reproduction 16, 1030-6.

Li X, Teske S, Conroy-Ben O. 2015. Estrogen mimics induce genes encoding chemical efflux proteins in gram-negative bacteria. Chemosphere 128, 327-331.

Lillywhite HB, Zippel KC, Farrell AP. 1999. Resting and maximal heart rates in ectothermic vertebrates. Comparative Biochemistry and Physiology 124, 369-382.

Martin PM, Berthois Y, Sasco A. 2004. Xenoestrogens, environmental estrogens, endocrine disruptors. 26th days of the SFSPM, Nancy.

Mikkelsen M, Jenssen BM. 2006. Polychlorinated biphenyls, sex steroid hormones and liver reinoids in adult male European common frogs (Rana temporaria). Chemosphere 63, 707-715.

Mückter H. 2003. What is toxicology and how does toxicity occur?. Best Practice and Research Clinical Anaesthesiology 17, 5–27.

Nash J, Kime DE, Van der ven L, Wester P, Brion F, Maack G, Stahlschmidt-Allner P, Tyler CR. 2004. Environmentally relevant concentrations of the pharmaceutical, ethinyloestradiol, impact fish populations. Environmental Health Perspectives 112, 1725-33.

Paro R, Tiboni GM, Buccione R, Rossi G, Cellini V, Canipari R, Cecconi S. 2012. The fungicide Mancozeb induces toxic effects on mammalian granulosa cells. Toxicology and Applied Pharmacology 260, 155–161.

Petit F, Le Goff P, Cravedi JP, Valotaire Y, Pakdel F. 1997. Two complementary bioassays for screening the estrogenic potency of xenobiotics: recombinant yeast for trout estrogen receptor and trout hepatocyte cultures. Journal of Molecular Endocrinology 19, 321-35.

Petridis P, Awadhesh N, William J, Langston. 2009.  Measurements  of  the  genotoxic  potential  of (xeno-) oestrogens in the bivalve mollusc Scrobicularia plana, using the Comet assay. Chemosphere 128, 327-331.

PPDB. 2014. Pesticide Mancozeb. Properties DataBase, University Hertfordshire.

Schonweger G, Schwerte T, Pelster B. 2000. Temperature-dependent development of cardiac activity in unrestrained larvae of the minnow Phoxinus-Phoxinus. American Journal of Physiology Regulatory Integrative and Comparatie Physiology 279, 1634-40.

Simmaco M, Mignogna G, Barra D. 1998. Antimicrobial peptides from amphibian skin: What do they tell us?. Biopolymers (Peptide Science) 47, 435-450.

Sparling DW, Linder G, Bishop CA. 2000. Ecotoxicology of Amphibians and Reptiles. In: SETAC Press, Pensacola, FL, 325.

Trotti R, Carratelli M, Barbieri M. 2002. Performance and clinical application of a new, fast method for the detection of hydroperoxides in serum. Panminerva Medica 44, 37–40.

Yu S, Wages MR, Cobb GP, Maul JD. 2013. Effects of chlorothalonil on development and growth of amphibian embryos and larvae. Environmental Pollution 181, 329-34.

Yoon CK, Jin JH, Park JH, Youn HJ, Cheong SW. 2003. The fungicide benomyl inhibits differentiation of neural tissue in the Xenopus embryo and animal cap explants. Environmental Toxicology 18, 327–337.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background