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Transformation of signal sequence in Escherichia coli by reporter gene fusion

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Research Paper 01/08/2011
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Transformation of signal sequence in Escherichia coli by reporter gene fusion

Ahmad Humayan Kabir, FM Ali Haydar, M Shibli Abedin
Int. J. Biomol. & Biomed.1( 2), 27-31, August 2011.
Certificate: IJBB 2011 [Generate Certificate]
Key: Erwinia carotovoraGene fusionpHSK34 plasmidSignal sequence

Abstract

Transformation of signal sequence by reporter gene fusion technology was attempted. The promoter containing signal sequences was taken from Erwinia carotovora, a plant pathogenic soil bacterium. Plasmid DNA (Deoxyribonucleic acid) and genomic DNA were cleaved by BamHI and Sau3A1, respectively. Result of gel electrophoresis reveals successful cutting of restriction enzyme in both plasmid and genomic DNA. Best banding was found in 0.75 U/mg DNA of Sau3A1 compared to other concentrations of Sau3A1 used. Bacterial growth was found on LBcmp (Luria-Bertani) plates, since chloramphenicol resistance ability was pre-existed in this plasmid. Highest colony forming unit was observed with ligation ratio (1:5). It was also evident that the cells have been successfully transformed with the plasmid resulting notable growth of bacteria in LBamp plate.

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