User friendly DNA isolation protocol optimized for Ricinus communis L. seeds

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Research Paper 01/08/2013
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User friendly DNA isolation protocol optimized for Ricinus communis L. seeds

Ajmal Iqbal, Waqar Ahmad, Asaf Khan, Murad Khan, Mohammad Nisar
Int. J. Biosci.3( 8), 124-129, August 2013.
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A protocol was developed to isolate high quality genomic DNA from the seeds of Ricinus communis L. (caster) without using liquid nitrogen.The DNA extraction buffer used in this novel protocol constitutes SDS (1%), Tris (1.21%), NaCl (0.58%), EDTA (0.32%), 0.12% and β–Mercaptoethanol witha pH 8.5. In the protocol 0.09g of crushed seeds of caster bean, 600ul of DNA extraction buffer and and 500ul of phenol: chloroform: iso-amylalcohol with a ratio of 25:24:1 were used. The isolated DNA was amplified in Polymerase Chain Reaction using RAPD and SSR primer sets. The primer set successfully amplified the isolated DNA. Hence, the protocol is recommended as a user friendly novel protocol for DNA isolation from the castor beans seeds.


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