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Uterotrophic activities of the aqueous extract of Moringa oleifera (Moringaceae) Lam. 1785 on young ovariectomized rats

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Research Paper 12/12/2023
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Uterotrophic activities of the aqueous extract of Moringa oleifera (Moringaceae) Lam. 1785 on young ovariectomized rats

Kouakou Koffi Roger, Affy Mataphouet Emmanuel Guy Jocelin, Kablan Kassi Jean-Jacques, Tahiri Annick
Int. J. Biosci.23( 6), 196-207, December 2023.
Certificate: IJB 2023 [Generate Certificate]
Key: Adrenal glandsOvariectomyRatsTrophic uterusUterus

Abstract

Studies have been carried out to determine the estrogenic activity of Moringa oleifera. However, less data exists on the uterotrophic effect of the extract of this plant. This study aims to evaluate the uterotrophic effects of the aqueous extract of Moringa oleifera in young ovariectomized rats. The plant material consists of Moringa oleifera leaves and the animal material consists of Rattus norvegicus rats. Young rats underwent bilateral ovariectomy according to the guideline of the Organization for Economic Co-operation and Development. Batch 1 received distilled water. The animals of batches 2, 3, 4 and 5 received respectively olive oil, 17β-estradiol at 2.10-2 mg/Kg of body weight, doses of 150 and 300 mg/Kg of body weight of aqueous extract of Moringa oleifera. Lots 6 and 7 respectively received doses of 150 and 300 mg/Kg body weight of extract, each associated with a dose of 2.10-2 mg/Kg body weight of 17β-estradiol. The animals were weighed during the seven days of treatment. The fresh and dry weights of rats experienced a significant increase (p<0.05) only with 17β-estradiol at 2.10-2 mg/Kg bw, doses of 150 and 300 mg/Kg bw of aqueous extract of M. oleifera each associated with the dose of 2.10-2 mg/Kg of body weight of 17β-estradiol compared (p<0.05) to those of the respective controls. Moringa oleifera appears to have uterotrophic effects on young ovariectomized rats after seven days of treatment.

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Aké-Assi L. 2002. Flore de la Côte d’Ivoire 2, catalogue systématique, biogéographique et écologie. Conservatoire et Jardin Botanique. Genève (Suisse). 401 p.

Blahi A, Nadia M, Zougrou NE, Lavry GN, Kouakou K. 2017. Effects of the aqueous extract of Sarcocephalus latifolius (smith) sheets on the reproduction parameters in male rats. The Pharma Innovation Journal 6(6), 85-91.

Bleu GM, Kouakou K, Zahoui OS, Touré A, Traoré F. 2012. Oral acute toxicity and estrogenic effects of the extracts of Passiflora foetida Linn. (Passifloraceae) leaves in female Wistar albino rats. Annals of Biological Research 3(9), 4609-4616.

Butera PC. 2010. Œstradiol and the control of food intake. Physiology & Behavior 99(2), 175-180.

Cekmez F, Canpolat FE, Pirgon O, Çetinkaya M, Aydinoz S, Suleymanoglu S. 2011. Apelin, vaspin, visfatin and adiponectin in large for gestational age infants with insulin resistance. Cytokine 56, 387-391.

Clarke R, Liu MC, Bouker KB, Gu Z, Lee RY, Zhu Y, Skaar TC, Gomez B, O’brien K, Wang Y, Hilakivi-Clarke LA. 2003. Antioestrogen resistence in breast cancer and the role of estrogen receptor signaling. Oncogene 22(47), 7316-7339.

Dabhadkar DK, Zade VS, Rohankar PH, Pare SR, Wikhe MA. 2012. Estrogenic and anti-estrogenic potentials of ethanolic pod extract of Plumeria rubra in female albino rats. Global Journal of Pharmacology 6, 142-147.

Diel P, Schmidt S, Vollmer G. 2002. In vivo test systems for the quantitative and qualitative analysis of the biological activity of phytoestrogens. Journal of Chromatography B. 777, 191-202.

Dinesh KD, Varsha SZ, Pratibha HR, Shital RP, Manisha AW. 2012. Estrogenic and anti-estrogenic potentials of ethanolic pod extract of Plumeria rubra in female albino rats. Global Journal of Pharmacology 6 (2), 142-147.

Dorfman RI, Gallagher TF, Koch FC. 1936. The nature of the estrogenic substance in human male urine and bull testis. Endocrinology 19, 33 – 41.

Fatemeh PS, Mojdeh S, Mehdi FM, Mojtaba RV, Ebrahim H. 2008. The changes in morphology and morphometrical indices of endometrium of ovariectomized mice in response to exogenous ovarian hormones. Iranian Journal of Reproductive Medicine 6, 125-131.

Heryanto B. et Rogers P. A. 2002. Regulation of endometrial cell proliferation by oestrogen and progesterone in the ovariectomized mouse. Reproduction. 123: 107-113.

Hiremath SP, Hanumanthara S. 1990. Antifrtility activity of striga lutea (Scrophulariaceae) on rats. Contraception 42, 466-477.

Horne FM, Blithe DL. 2007. Progesterone receptor modulators and the endometrium: changes and consequences. Human Reproduction Update 13, 567-580.

Hould R. 1984. Techniques d’histopathologie et de cytopathologie. Editor: Centre collégial de développement de matériel didactique. Québec (Province). 399p.

Huet-Hudson YM, Andrews GK, Dey SK. 1989. Cell typespecific localization of c-myc protein in the mouse uterus: modulation by steroid hormones and analysis of the periimplantation period. Endocrinology 125, 1683-1690.

Jacob D, Morris JM. 1969. Estrogenic activity of postcoital antifertility compounds. Fertility and Sterility 20, 211-222.

Jensen EV, Desombre ER. 1972. Mechanism of action of the female sex hormones. Annual Review of Biochemistry 203-230.

Kouakou K, Kati CS, Mangue NEJ. 2008. Effets bénéfiques des extraits d’Arachis hypogaea (Fabacée) et de Phaseolus vulgaris (Fabacée) sur la perte osseuse chez un modèle animal d’ostéoporose 6(1), 5-12.

Kouakou K, Benié T. 2003. Effet antifertilisant de Daldinia concentrica et Psathyrella efflorescens. Recherche des effets oestrogéniques. Ethnopharmacologia 31, 45-57.

Kouakou KR,  Tahiri A, Kouakou K. 2018. Effect of aqueous extract of leaves of Moringa oleifera (Moringaceae) LAM. 1785 on the reproductive cycle of animal model Rattus norvegicus (Murine) wistar strain. European Journal of Biotechnology and Bioscience 6(6), 37-42.

Kozvun OI. 1996. The effect of ovariectomy and œstradiol on DNA, RNA and protein metabolism in adrenal cortex of rats. Fiziologichnyi Zhurnal 42(1), 53-58.

Lessy BA. 2003. Two pathways of progesterone action in the human endometrium: implications for implantation and contraception. Steroids 68, 809-815.

Ljungkvist I. 1971. Attachment reaction of rat uterine luminal epithelium. The effect of œstradiol estrone and estriol on the morphology of the luminal epithelium of spayed virgin rats. Acta of the Society of Medicine Uppsala 76, 139- 157.

Ljungkvist I. 1971. Attachment reaction of rat uterine luminal epithelium. The effect of œstradiol estrone and estriol on the morphology of the luminal epithelium of spayed virgin rats. Acta of the Society of Medicine Uppsala 76, 139- 157.

Murray MK. 1992. The effect of estrogen and progesterone on structural changes in the uterine glandular epithelium of the ovariectomized sheep. Biology of Reproduction 47, 408-417.

Obuchi GO, Malu SP, Obi-Abang M, Alozie Y, Iyam MA. 2009. Effect of garlic extracts on Monosodium Glutamate (MSG) Induced fibroid in Wister rats. Science Alert 8(7), 970-976.

OCDE. 2008. Bioessai utérotrophique chez les rongeurs: un test de dépistage à court terme pour les propriétés œstrogéniques. 21p.

Panda S, Kar A, Sharma P, Sharma A. 2013. Cardioprotective potential of N,α-Lrhamnopyranosyl vincosamide, an indole alkaloid, isolated from the leaves of Moringa oleifera in isoproterenol induced cardiotoxic rats: In vivo and in vitro studies. Bioorganic and Medicinal Chemistry Letters 23(4), 459-462.

Philippi C. 1997. Moringa l’arbre aux usages Multiples. Thèse de Docteur d’Etat en Pharmacie. Université Henri Poincaré – Nancy I. 178p.

Pollard RM, Finn CA. 1974. The effect of ovariectomy of puberty on cell proliferation and differentiation in the endometrium of the aged mouse. Biology of Reproduction 10, 74-77.

Psychoyos A. 1966. Recent research on egg implantation, CIBA Foundation Study Group. 23 p.

Psychoyos A, Prapas I. 1987. Inhibition of egg development and implantation in rats after post­coital administration of the progestrerone antagonist RU 486. Journal of Reproduction and Fertility 80, 487-491.

Purwal L, Pathak AK, Jain UK. 2010. In vivo anticancer activity of the leaves and fruits of Moringa oleifera on mouse melanoma. Pharmacologyonline 1, 655-665.

Reel JR, Lamb JC, Neal BH. 1996. Survey and assessment of mammalian estrogen biological assays for hazard characterization. Fundamental and Applied Toxicology 34, 288-305.

Roberts AB, Sporn MB. 1992. Mechanistic interrelationships between two superfamilies: the steroid/retinod receptors and transforming growth factor-b. Cancer Surveys 14, 205-220.

Saengsirisuwan V, Pongseeda S, Prasannarong M, Vichaiwong K, Toskulkao C. 2009. Modulation of insulin resistance in ovariectomized rats by endurance exercise training and estrogen replacement. Metabolism 58(1), 38-47.

Shaila G, Mahjan, Anita A. 2010. Immunosuppresive activity of ethanolic extract of seeds of Moringa oleifera Lam in experimental immune inflammation. Ethnopharmacol Commun 130, 183–186.

Sripriya S, Yuvaraj G, Nema RK, Kumar VM, Deecaraman M. 2011. Evaluation of Antifertility activity from Stem Part of Ocimum gratissimum in Acetone extracts. International Journal of Pharmaceutical and Clinical Research 3(2), 41-44.

Sylvie D, Claudine B, Isabelle M. 2003. Phytoestrogènes chez la femme ménopausée. Medecine/Sciences 19, 1030-1038.

Terada N, Yamamoto R, Takada T, Miyake T, Terakawa N, Wakimoto H, Taniguchi H. 1989. Inhibitory effects of progesterone on cell death of mouse uterine epithelium. Journal of Steroid Biochemistry 33, 1091-1096.

Touré D. 2015. Etudes chimique et biologique des huiles essentielles de quatre plantes aromatiques medicinales de côte d’ivoire. Thèse de Doctorat en Biologie Humaine Tropicale. Université Félix Houphouët- Boigny Abidjan. Archives-ouvertes, 116p.

Yang R, Lien-Chung C, Jenn-Chung H, Weng BC, Palada CM, Chadha ML, Levasseur V. 2006. Nutritional and functional properties of Moringa leaves from germplasm, to plant, to food, to health. Strategies, standards and markets for a better impact on nutrition in Africa. Accra, (Ghana). 16-18.

Zirihi GN, Kra AM, Guédé-Guina F. 2003. Évaluation de l’activité antifongique de Microglossa pyrifolia (LAMARCK) O. KUNZE (ASTERACEAE) << PYMI>> sur la croissance in vitro de Candida albicans. Revue de méecines et pharmacopés africaines 17 11-18.

Zoth N, Weigt C, Laudenbach-Leschowski U, Diel P. 2010. Physical activity and estrogen treatment reduce visceral body fat and serumlevels of leptin in an additive manner in a diet induced animal model of obesity. Journal of Steroid Biochemistry and Molecular Biology 122, 100-105.

Zougrou NE, Blahi AN, Kouassi KD, Kouakou K. 2018. Effects of the Aqueous Extract of Cnestis Ferruginea on the Histological Structure of Female Rat Ovary and Uterine Horns. Research. Biomed J Sciences & Technical. 2(1), 1-6.

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