Vitamin C retards the growth and motility of liver cancer cells huh 7 in wound healing assay

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Research Paper 01/10/2017
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Vitamin C retards the growth and motility of liver cancer cells huh 7 in wound healing assay

Muhammad Imran Raza, Sara Hassan, Wasifa Naushad, Hajra Sadia
Int. J. Biosci.11( 4), 359-364, October 2017.
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Vitamin C has long been discussed as having anticancer potential but with little evidence on mechanism of action. Some clinical studies have been performed but with no conclusive results. Researchers have been calling for more studies. The aim of study was to investigate the anti-cancer role of high dose Vitamin C on growth and motility of Liver cancer cells Huh 7 in wound healing Assay. Huh-7 cells were cultured in complete medium (DMEM) at 37oC with 5% CO2. For experimental study design, Huh 7 cells were divided in to two groups as: cells treated with Vitamin C and cell without Vitamin C treatment. To assess the viability of cancer cells upon vitamin C treatment, MTT assay was done. For growth kinetics, cells were plated in a 6 well plate and counted using haematocytometer at different time points. Cancer cell motility was assessed thorough two-dimensional migration wound healing assay. Statistical analysis was done using Graph-pad prism software. Vitamin C treatment inhibits the growth and motility of liver cancer cells. Percentage of wound closure in untreated is high as compared to Vitamin C treated cells which shows Vitamin C significantly retards the growth of Cancer cells (P<0.05). Vitamin C inhibits growth and motility of liver Cancer cells Huh-7 in wound healing assay.


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