Paper Details

Volatile constituents of Polylophium involucratum, a wild plant grown in Iran , extracted by HS-SPME

Sahar Kashipaz Ha,  Fereshteh Nematollahi

DOI: https://dx.doi.org/10.12692/ijb/6.6.68-71

Int. J. Biosci. 6(6), 68-71. March, 2015. (PDF)

Abstract:

The volatile constituents of Polylophium Involucratum , which grown wild in the north of Iran, were extracted by head-space solid phase microextraction using a 65 μm dimethyl siloxane- divinyl benzene (PDMS-DVB) fiber in order to determine compounds. The extracted compounds were analyzed by means of gas chromatography (GC) and gas chromatography- mass spectroscopy (GC-MS). – terpinene (20.71%), sabinene (14.86%), p- cymene (12.91%) and bornyl angelate (10.2%) were the major components in volatile fraction of P. Involucratum. Monoterpene. Hydrocarbons were the major compounds in head space of P.Involucratum. The main aim of this study is the extraction of volatile constituent of plants with an affordable method that is suitable for heat sensitive components. HS-SPME method, which needs no solvent, can be used as a Superseded method instead of hydrodistillation, due to less chemical changes in volatile composition, especially in the case of oxygenated ones, of plants.