Wound healing potency of Scratch assay and MTT assay on whole plant methanol extract of Parkinsonia aculeata L.

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Research Paper 15/07/2023
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Wound healing potency of Scratch assay and MTT assay on whole plant methanol extract of Parkinsonia aculeata L.

Mahesha N., Shrishail
Int. J. Biosci.23( 1), 276-283, July 2023.
Certificate: IJB 2023 [Generate Certificate]

Abstract

Traditional practice for curing diseases using locally available medicinal plants is a culture adopted by Indians from prehistoric period. Leguminosae members are also used as medicine for various diseases; one among them is P. aculeata. This is selected for the study.  Identification and assessment of therapeutic potential of nature products derived from medicinal plants have led to the discovery of innovate and economical drugs to treat several diseases, which includes chronic wounds. In vitro cell based scratch assay is an appropriate and inexpensive method for initial understanding of wound healing potential of medicinal plant extracts. The current study was aimed at investigating the wound healing capacity of P. aculeata whole plant methanol extract by using scratch assay as a primary model, where proliferative and migratory capability of test compounds could be monitored through microscopy studies. Methanol extraction of the plant material was done using Soxhlet apparatus and the cytotoxicity of the extract during 2021-22 cells was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheyltetrazolium bromide (MTT) assay. In vitro scratch assay was performed by flow cytometric expression studies of an extracellular matrix (ECM) factor, L2 cell-line for 24hrs. MTT assay revealed that P. aculeata whole plant methanol extract had no cytotoxic effect on the cells and at higher concentration, the extract that showed mild toxicity resulting in the death of just 10% cells. Scratch assay showed 50, 100, 150, 200 and 250 concentrated wound closures at 0hrs, 12hrs and 24hrs of incubation respectively. These results were similar compared to positive control which showed 100, 85.83, 92.12, 85.62, 71.98 and 63.83% of wound closure. Further, flow cytometry-based studies revealed that the P. aculeata whole plant extract induced the expression of DMEM remodeling factor L2 cell-line. Our study revealed the wound healing capabilities of P. aculeata In vitro. Hence, P. aculeata could be recommended as a potential source of wound healing agent.

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