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Wsp gene based detection and characterization of Wolbachia in indigenous Drosophila

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Research Paper 01/04/2017
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Wsp gene based detection and characterization of Wolbachia in indigenous Drosophila

Muhammad Sajjad Sarwar, Nusrat Jahan, Falak Batool, Bushra Kalim
J. Bio. Env. Sci.10( 4), 142-149, April 2017.
Certificate: JBES 2017 [Generate Certificate]
Key: DetectionDrosophilaPakistanWolbachiaWolbachia surface protein (wsp)

Abstract

Wolbachia is a genus of intracellular symbiotic bacteria, vertically transmitted in more than 60% insects. Wolbachia interferes with host physiology and induces various alterations like cytoplasmic incompatibilty, parthenogenesis, feminization, male killing and inhibition of parasite and pathogen propagation. In last decade, it has been used as transinfected bioagent in the control strategies of vector-borne diseases. Current study involves the detection of Wolbachia from Drosophila melanogaster and D. simulans for the first time in Pakistan. The fruit flies collected were screened for the presence of Wolbachia using Wolbachia surface protein (wsp) gene specific primers by conventional polymerase chain reaction (PCR). D. melanogaster and D. simulans represented 90.6% (96/106) and 89.6% (69/77) Wolbachia infection respectively. Nucleotide BLAST reslut of partial wsp gene sequence from detected strain of D. melanogaster indicated 100% homology with Walachia endosymbiont of D. melanogaster reported from Australia and detected Wolbachia of D. simulans represented 100% homology with Wolbachia endosymbiont wRi strain of D. simulans from Sweden. Both of the detected strains belonged to supergroup A and were named as wMel (D. melanogaster) and wRiv (D. simulans) on the basis of host and wsp similarity.

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