The effects of co-inoculation of PGPR bacteria and Sinorhizobium meliloti on nutrient contents, plant growth and yield of alfalfa

Paper Details

Research Paper 01/11/2019
Views (834)
current_issue_feature_image
publication_file

The effects of co-inoculation of PGPR bacteria and Sinorhizobium meliloti on nutrient contents, plant growth and yield of alfalfa

Alireza Tavasolee, Kazem Khavazi, Ahmad Asgharzadeh, Hassan Monirifar, Saeid Ghassemi
J. Biodiv. & Environ. Sci. 15(5), 8-13, November 2019.
Copyright Statement: Copyright 2019; The Author(s).
License: CC BY-NC 4.0

Abstract

Two field experiments with factorial arrangement based on a randomized complete block design with three replications were conducted in 2013 and 2014 to evaluate the effects of co-inoculation of PGPR bacteria (control and mixed of azotobacter, azospirillum and pseudomonas inoculations) and Sinorhizobium meliloti (control, strain 1, strain 2, strain 3 and mixed of these strains) on nutrient contents, plant growth and yield of alfalfa (Medicago sativa). Results showed that application of PGPR bacteria enhanced nitrogen (N), phosphorus (P), manganese (Mn), zinc (Zn) and copper (Cu) contents of alfalfa in compared with control. Plant height, fresh and dry weight of forage in 2013 were significantly higher than 2014. PGPR treatment significantly increased plant height, number of branches, fresh and dry weight of forage of alfalfa compared with control.

Arshad MF, Frankenberger WT. 1998. Plant growth-regulating substances in the rhizosphere: microbial production and functions. Advances in Agronomy 62, 45-51.

Babalola OO. 2010. Beneficial bacteria of agricultural importance. Biotechnology Letters 32, 1559-1570.

Brewin NJ. 2004. Plant cell wall remodelling in the Rhizobium-legume symbiosis. Critical Reviews in Plant Sciences 25, 1-24.

Cappellari L, Chiappero J, Valeria Santoro M, Giordano W, Banchio E. 2017. Inducing phenolic production and volatile organic compounds emission by inoculating Mentha piperita with plant growth-promoting rhizobacteria. Scientia Horticulturae  220, 193-198.

Chanway CP. 2002. Plant growth promotion by Bacillus and relatives. In: Berkeley, R., Heyndrickx, M., Logan, N., De Vos, P. (Eds.), B. subtilis for Biocontrol in Variety of Plants. Blackwell Publishing, Malden, MA, pp. 219-235.

Cleland R. 1971. Cell wall extension. Annual Review of Plant Physiology 22, 197-222.

Esitken A, Ercisli S, Karlidag H, Sahin F. 2005. Potential use of plant growth promoting rhizobacteria (PGPR) in organic apricot production. In: Proceedings of the International Scientific Conference of Environmentally Friendly Fruit Growing, Tartu-Estonia pp. 90-97.

Ghassemi-Golezani K, Zafarani-Moattar P, Raey Y, Mohammadi M. 2010. Response of pinto bean cultivars to water deficit at reproductive stages. Journal of Food, Agriculture and Environment 8, 801-804.

Gibson KE, Kobayashi H, Walker GC. 2008. Molecular determinants of a symbiotic chronic infection. Annual Review of Genetics 42, 413-441.

Glick BR, Changping L, Sibdas G, Dumbroff EB. 1997. Early development of canola seedlings in the presence of the plant growth promoting rhizobacteria Pseudomonas putida GR12-2. Soil Biology and Biochemistry 29, 1233-1239.

Hearn AB. 1994. The principles of cotton water relations and their application in management, in: Constable, G.A., Forrester, N.W. (Eds.), Challenging the Future, Proc. World Cotton Conf. Brisbane, Australia pp. 66-92.

Holl FB, Chanway CP, Turkington R, Radley RA. 1988. Response of crested wheatgrass (Agropyron cristatum L.), perennial ryegrass (Lolium perenne L.), and white clover (Trifolium repens L.) to inoculation with Bacillus polymyxa. Soil Biology and Biochemistry 20, 19-24.

Hu YG, Cash D. 2009. Global status and development trends of alfalfa. In alfalfa management guide for Ningxia. United Nations Food and Agriculture Organization. Beining, China.

Jiang HM, Jiang JP, Jia Y, Li FM, Xu JZ. 2006. Soil carbon pool and effects of soil fertility in seeded alfalfa fields on the semi-arid Loess Plateau in China, Soil Biology and Biochemistry 38, 2350-2358.

Lindberg T, Granhall U. 1984. Isolation and characterization of dinitrogen-fixing bacteria from the rhizosphere of temperate cereals and forage grasses. Applied and Environmental Microbiology 48, 683-689.

Marschner H. 1995. Mineral Nutrition of Higher Plants, 2nd ed. Academic Press, London.

O’Connell PF. 1992. Sustainable agriculture valid alternative. Outlook on Agriculture 21, 5-12.

Pérez-Montano F, Alías-Villegas C, Bellogín RA, del Cerro P, Espuny MR, Jiménez-Guerrero I, López-Baena FJ, Ollero FJ, Cubo T. 2014. Plant growth promotion in cereal and leguminous agriculturalimportant plants: From microorganism capacities to crop production. Microbiological Research 169, 325-336.

Shao HB, Chu LY, Jaleel CA, Zhao CX. 2008. Water-deficit stress-induced anatomical changes in higher plants. Comptes Rendus Biologies 331, 215-225.

Sprent JI. 2001. Nodulation in Legumes. London: Royal Botanic Gardens, Kew.

Related Articles

Value chain analysis and business model development of the Rizal mango growers agriculture cooperative

Macluven T. Gonzales, Diana O. Lim*, Karen Joy A. Abalos, J. Biodiv. & Environ. Sci. 29(1), 186-198, July 2026.

Susceptibility of Culex quinquefasciatus to insecticides in the industrial areas of Koumassi, Vridi, and Yopougon in Abidjan, Ivory Coast, during the rainy season

Gahapie Urbain Silue*, Koffi Ladji Yao, N’tamon Romeo N’tamon, Edmond Charles Basseli, Genevieve Lydie Acapovi-Yao, Yao Lucien Konan, J. Biodiv. & Environ. Sci. 29(1), 175-185, July 2026.

Optimization of maltodextrin as a bulking agent in mango (Mangifera indica) fruit bar: Physical, sensory, and nutritional evaluation

Macluven T. Gonzales*, Diana O. Lim, Jocelyn D. Tuliao, Hitler C. Dangatan, J. Biodiv. & Environ. Sci. 29(1), 150-163, July 2026.

An assessment of the effect of wind flow on the indoor thermal condition of some buildings in Benin City, Nigeria

Tashok, Yusuf Haruna*, J. Biodiv. & Environ. Sci. 29(1), 136-149, July 2026.

Analyzing roadside tree diversity reveals dominance, shadow diversity, and management archetypes: The case of Tagbilaran City, Bohol, Philippines

Johnuel Tac-on, Jesie Diola, Noel T. Lomosbog, Jairyl B. Oclarit, MA. Aneli A. Lanzaderas*, J. Biodiv. & Environ. Sci. 29(1), 122-135, July 2026.

Floristic diversity of major river systems in Zamboanga del Norte

Ma. Dulce C. Guillena*, Maria Rio A. Naguit, Ethel T. Cata-al, Tessie G. Pulido, J. Biodiv. & Environ. Sci. 29(1), 101-111, July 2026.