Optimization of laboratory requirements through experimental design for maximum growth of indigenous saccharomyces cerevisiae using apple waste as substrate
Paper Details
Optimization of laboratory requirements through experimental design for maximum growth of indigenous saccharomyces cerevisiae using apple waste as substrate
Abstract
From ancient wild type Saccharomyces Cerevisiae is used for production of valuable products. These microorganisms can be grown on a number of carbohydrate rich waste materials. For optimum valuable products It is needed to optimize different parameters for growth of indigenous S. cerevisiae utilizing apple waste. For the said purpose Indigenous S. cerevisiae was isolated from different fruit samples and identified by Polymerase chain reaction (PCR). Apple waste was collected and chemically treated to convert complex polysaccharide into simple one. For optimum growth, different laboratory parameters i.e.pH, temperature, shaking and glucose concentration were optimized using response surface methodology. Dry microbial biomass was analyzed for proximate composition i.e. crude protein, crude fibers, crude fats, total carbohydrates and ash contents. Dry microbial biomass was also evaluated for the presence of different amino acids through aminoacid analyzer using orthophthalaldehyde (OPA) as a fluorescent agent. Results revealed that 2.7% glucose, 32oC temperature, pH 5 and shaking at 150 rpm were best for optimum growth of indigenous S. cerevisiae. Dry microbial biomass was rich in crude proteins (44.65%) followed by carbohydrate (43.09%). It was observed that dry microbial biomass was rich in aspartic acid and leucine (14.57%) each, followed by serine (12.89%) and alanine (11.37%).From the present study it is concluded that using response surface methodology different growth parameters can be optimized for indigenous S. cerevisiae on apple waste. Dry microbial biomass is rich in crude protein and essential amino acids therefore it can be used as a source of single cell protein.
Association of Official Analytical Chemist. 2006. The official methods of analysis of AOAC international. 18th edition Ed.; The Association of Official Analytical Chemists Arlington, U.S.A.
Asad MJ, Asghar M, Yaqub M, Shahzad K. 2000. Production of single cell protein from delignified corn cob by Arachniotus species. Pakistan Journal of Agricultural Sciences 37, 3-4.
Bacha U, Nasir M, Khalique A, Anjum A, Jabbar M. 2011. Comparative assessment of various agro-industrial wastes for Saccharomyces cerevisiae biomass production and its quality evaluation as single cell protein. Journal of Animal and Plant Sciences 21, 844-849.
Barnett JA, Payne RW, Yarrow D. 1983. Yeasts: Characteristics and identification Cambridge University Press.
Bekatorou A, Psarianos C, Koutinas AA. 2006. Production of food grade yeasts. Food Technology and Biotechnology 44, 407-415.
Fred E, Peterson W. 1921. Fermentation Process for the Production of Acetic and Lactic Acids from Corncobs. Industrial & Engineering Chemistry 13, 211-213.
Haddadin MS, Abdulrahim SM, Al‐Khawaldeh GY, Robinson RK. 1999. Solid state fermentation of waste pomace from olive processing. Journal of Chemical Technology and Biotechnology 74, 613-618.
Imrie F, Righelato R. 1976. Production of microbial protein from carbohydrate wastes in developing countries. Food from waste 79-94.
Ishida Y, Fujita T, Asai K. 1981. New detection and separation method for amino acids by high-performance liquid chromatography. Journal of Chromatography A 204, 143-148.
Jamal P, Alam M, Salleh N. 2008. Media optimization for bioproteins production from cheaper carbon source. Journal of Engineering Science and Technology 3, 124-130.
Kihlberg R. 1972. The microbe as a source of food. Annual Reviews in Microbiology 26, 427-466.
Kurtzman C, Fell JW, Boekhout T. 2011. The yeasts: a taxonomic study: Elsevier.
Li C, Bai J, Cai Z, Ouyang F. 2002. Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology. Journal of Biotechnology 93, 27-34.
Martorell P, Querol A, Fernández-Espinar M. 2005. Rapid identification and enumeration of Saccharomyces cerevisiae cells in wine by real-time PCR. Applied and environmental microbiology 71, 6823-6830.
Ojokoh A, Uzeh R. 2005. Production of Saccharomyces cerevisiae biomass in papaya extract medium. African Journal of Biotechnology 4, 1281-1284.
Paul D, Mukhopadhyay R, Chatterjee BP, Guha AK. 2002. Nutritional profile of food yeast Kluyveromyces fragilis biomass grown on whey. Applied biochemistry and biotechnology 97, 209-218.
Ramı́Rez J, Gutierrez H, Gschaedler A. 2001. Optimization of astaxanthin production by Phaffia rhodozyma through factorial design and response surface methodology. Journal of Biotechnology 88, 259-268.
Saima M, Akhter M, Khan U. 2008. Investigation on the availability of amino acids from different animal protein sources in golden cockerels. The Journal of Animal and Plant Sciences18, 53-56.
Sivasankar B. 2002. Food processing and preservation: PHI Learning Pvt. Ltd.
Tannenbaum SR, Wang DI. 1975. Single-cell protein II: MIT Press.
Vazquez M, Martin AM. 1998. Optimization of Phaffia rhodozyma continuous culture through response surface methodology. Biotechnology and bioengineering 57,314-320.
Faheem Ahmed Khan, Sarzamin Khan, Nafees Bacha, Tariq Khan, 2018. Optimization of laboratory requirements through experimental design for maximum growth of indigenous saccharomyces cerevisiae using apple waste as substrate. Int. J. Biosci., 12(1), 136-142.
Copyright © 2018 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.