home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Histological evaluation of the thymocyte composition in the thymus gland of mice by using soft laser

Paper Details

Research Paper 01/07/2018
Views (320) Download (33)
current_issue_feature_image
publication_file

Histological evaluation of the thymocyte composition in the thymus gland of mice by using soft laser

Ahmed Anwar Albir
Int. J. Biosci.13( 1), 65-71, July 2018.
Certificate: IJB 2018 [Generate Certificate]
Key: Histological alterationsMedical LaserMiceThymocytes of the thymus gland

Abstract

Background: Thymus is composed of two lobes and it is located in the mediastinum, each one is split up into many lobules and each of them is separated from another by trabeculae that consist of connective tissue. The author used thymus gland as a target for soft laser in order to achieve better results due to hypothetical effects on its composition mainly thymocytes.The presented research was aimed to find the possible effect of soft laser on the cellular composition of the normal thymus gland of mice. Thirty three of normal male from Swiss albino mice at 9 weeks of age and body weight 41-49 gm were the targets for this experimental study. These mice were separated into three groups (11mice/group). The first group was kept as a control and was not subjected to radiation of the laser, while the rest of the groups were exposed to laser irradiation. The outcomes of this study show how laser caused marked alterations in the cellular contents of the thymus glands of the second group of mice mainly the thymocytes which included increased size of the thymocytes and multiplication of the cellular nuclei. The mentioned alterations were more plentiful in the thymocytes composition of the thymus glands of the last set of mice irradiated with laser. Soft laser proved the hypothesis and caused noticeable atterations in the thymocytes structure and that in turn could support the immune system as well.

VIEWS 13
Cover PDF

Junqueira LC, Carneiro J. 2003. Basic histology. Text & Atlas.10thedn.Chapter 14. Lange NY, 265-290.

Mescher AL. 2013.Junqueira’s Basic Histology. Text & Atlas 13thedn. Chapter 14. Lange NY, 262-288.

Elgbratt K. 2007. Characteristics and Consequences of Thymic Involution in Inflammatory Bowel Disease: Experimental studies in Gαi2-deficient and DSS-induced Colitis as well as in IBD patients. Thesis. Department of Microbiology and Immunology, Göteborg University.12, 13.

Prockop S, Petrie HT. 2000.Cell migration and the anatomic control of thymocyte precursor differentiation, Semin Immunol. 12,435-444.

Naquet P, Naspetti M,  Boyd R. 1999.Development, organization and function of the thymic medulla in normal, immunodeficient or autoimmune mice. Semin Immunol. 11, 47-55.

Wu L, Scollay R, Egerton M, Pearse M, Spangrude GJ, Shortman K. 1991.  CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus. Nature, 349(6304), 71-74.

Godfrey DI, Kennedy J, Suda T, Zlotnik A. 1993.A. developmental pathway involving four phenotypically and functionally distinct subsets of CD3-CD4-CD8-triple-negative adult mouse thymocytes defined by CD44 and CD25 expression. J. Immunol. 150(10), 4244-52.

Godfrey DI, Kennedy J, Mombaerts P, Tonegawa S, Zlotnik A. 1994.Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation. J. Immunol. 152(10), 4783-92.

vanEwijk W. 1991. T-cell differentiation is influenced by thymic micro environments. Annu. Rev, 9, 591-615.

Gill J, Malin M, Sutherland J, Gray D, Hollander G, Boyd R. 2003.Thymic generation and regeneration. Immunol. Rev. 195, 28-50.

Petrie HT. 2003. Cell migration and the control of postnatal T cell lymphopoiesis in the thymus. Nat. Rev. Immunol. 3, 859-866.

Aspinall R, Andrew D, Pido-Lopez J. 2002. Age- associated changes in thymopoiesis. Springer Semin. Immunopathol. 24(1), 87.

Taub DD, Longo DL. 2005. Insights into thymic aging and regeneration. Immunol.Rev. 205, 72.

Hamblin MR, Waynant RW.“Mechanisms for Low-Light Therapy.”Proc. of SPIE 6140614001-614010.

Gomes J, Velho L. 1997. Image Processing for Computer Graphics, Silvio Levy, New York, Inc.

Catão, MHCV. 2004. Osbeneficios do laser de baixa intensidade na clinica odontológica na estomatologia. Rev. Bras. Patol.Oral.3, 214-8.

Gomez-Villamandos RJ, Santisteban Valenzuela JM, Ruiz Calatrava I, Gomez-Villamandos JC, Avila Jurado I. 1995. He-Ne laser therapy by fibroendoscopy in the mucosa of the equine upper airway. Lasers Surg. Med. 16, 184-8.

Rocha JúniorAM, Vieira BJ, de Andrade LCF, Aarestrup FM. 2007. Effects of low-level laser therapy on the progress of wound healing in humans: the contribution of in vitro and in vivo experimental studies. J. Vasc. Bras.6, 258-266.

Submit Manuscript