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In vitro anticancer activity of small peptides from Tridax procumbens

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Research Paper 01/09/2018
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In vitro anticancer activity of small peptides from Tridax procumbens

Choppavarapu Hari Krishna, Kasturi Kondapalli
Int. J. Biosci.13( 3), 180-184, September 2018.
Certificate: IJB 2018 [Generate Certificate]
Key: Breast cancerCytotoxicitySmall peptidesT. procumbens

Abstract

Cancer is a hyper proliferative disorder and one of the most devastating diseases in both developing and developed countries. Chemotherapeutic drugs are enormously expensive and are associated with serious side effects. Still, the search continues for an ideal treatment that has minimal side effects and cost-effective. Over 50% of drugs in clinical trials for anti-cancer activity were isolated from natural plant sources. Plant species are enriched with potential anticancer proteins and peptides. Tridax procumbens phytochemicals having antiseptic, insecticidal, parasiticidal, anticancer properties, but the anticancer properties of peptides was not investigated. Thus the present investigation was aimed to evaluate anticancer activity of small peptides (SPs) from T. procumbens. The leaf, stem and flowers were shade dried and extracted with acetonitrile and formic acetic acid (50:1). The extracted SPs were purified by HPL Cand the purified SPs were tested for its cytotoxicity activity by MTT (-[3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyl tetrazolium bromide)] assay against human breast cancer metastatic cell line (MDA MB 231). Among all the tested SPs, leaf SPs showed lowest IC50with 4µg/ml. In conclusion, the SPs showed potent cytotoxicity against breast cancer which can be further characterised.

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