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Effect of temperature, pH and metal ions on amylase produced from selected indigenous extremophile bacteria in Pakistan

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Research Paper 01/09/2018
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Effect of temperature, pH and metal ions on amylase produced from selected indigenous extremophile bacteria in Pakistan

Farkhanda Kalsoom, Faisal Rasheed Anjum, Sidra Anam, Samiullah Khan, Fariha Hasan
Int. J. Biosci.13( 3), 262-275, September 2018.
Certificate: IJB 2018 [Generate Certificate]
Key: AmylaseExtremophileHalophilicOptimizationThermostable

Abstract

There is a burgeoning demand for amylase production due to wide range applications of amylase in different industrial processes like saccharification of starchy materials, food, detergents and textile industries. But high cost of fermentation media is one of the technical barriers in amylase production from microbial sources. Extremophiles microorganisms (thermophilic and halophilic) could be potential source for thermostable amylase. Present study deals with isolation of extremophilic amylase-producing bacteria from soil samples in starch agar medium and their subsequent identification through 16sRNA analysis. Different parameters like pH, temperature, and metal ions concentration (Ca+2, Mn+2, Fe+2, Zn+2) were optimized for amylase production. Five thermophilic strains and one halophilic strain were found positive for amylase production. Phylogenetic analysis showed that the amylase producing thermophilic strains includes Bacillus spp., Rheinheimera spp., Alishewanella spp., Pseudomonas spp., Microbacterium spp. while halophilic strain includes Bacillus spp. Thermophilic strains showed optimum amylase production at pH of 8 & 60°C, while maximum amylase activity for halophilic strains was observed at pH 7 and 40°C. Divalent ions Ca+2 and Mn+2 enhanced the amylase production while Zn and Fe did not a have any significant effect. Current research revealed that use of extremophile bacteria could be an important step towards the development of environmental friendly and cost effective process for thermostable amylase production.

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