Biodegradation of remazol black B by extracellular fungal laccase from Aspergillus oryzae AM1101

Paper Details

Research Paper 01/09/2018
Views (561)
current_issue_feature_image
publication_file

Biodegradation of remazol black B by extracellular fungal laccase from Aspergillus oryzae AM1101

Madiha Aftab, Arifa Tahir, Tayyaba Asim
Int. J. Biosci. 13(3), 290-296, September 2018.
Copyright Statement: Copyright 2018; The Author(s).
License: CC BY-NC 4.0

Abstract

Extensive use of reactive dyes in textile industries and their discharge in water bodies as industrial waste without any treatment causes major ecological damage. This study was concerned with the development of environment friendly method for degradation of toxic dye to allow reuse of textile waste water for agricultural and industrial purposes. In this study, biological degradation of Remazol Black B by crude laccase from batch culture of Aspergillus oryzae AM1101was investigated. Rice bran was used as a medium for laccase production. Decolorization potential of laccase was investigated at different initial dye concentration, temperature, enzyme concentration and pH. Results showed that by using 200µl of enzyme maximum decolorization (76%) of Remazol Black B was achieved at initial dye concentration of 50mg/L, temperature 50˚C and pH 4.0. It was concluded that development of cost effective and eco friendly processes for textile dye decolorization could be achieved by optimization of experimental conditions.

Alberts JF, Gelderblom WCA, Botha A, Vanzyl 2009. Degradation of aflatoxin B1 by fungal laccase enzymes. International Journal of Food Microbiology 135, 47–52. http://dx.doi.org/10.1016/j.ijfoodmicro.2009.07.022

Arulmani M, Aparanjini K, Vasanthi K, Arumugam P, Arivuchelvi M, Kalaichelvan TP. 2007. Purification and partial characterization of serine protease from thermostable alkalophilic Bacillus laterosporus AK1. World Journal of Microbiology and Biotechnology 23, 475-481. https://doi.org/10.1007/s11274-006-9249-7.

Birhanli E, Yesilada O. 2010. Enhanced production of laccase inrepeated-batch cultures of Funalia trogii and Trametes versicolor. Biochemical Engineering Journal 52, 33-37. https://doi.org/10.1016/j.bej.2010.06.019

Daassi D, Frikha F, Zouari-Mechichi H, Belbahri L, Woodward S, Mechichimize T. 2012. Application of response surface methodology to optimize decolorization of dyes by the laccase-mediator system. Journal of Environmental Managment 108, 84-91. https://doi.org/10.1016/j.jenvman.2012.04.039

Daâssi D, Zouari-Mechichi H, Frikha F, Martinez MJ, Nasri M, Mechichi T. 2013.  Decolorization of the azo dye Acid Orange 51 by laccase produced in solid culture of a newly isolated Trametes trogii strain. 3 Biotech 3(2), 115–125.  http://dx.doi.org/10.1007/s13205-012-0076-2.

El-Bakry M, Abraham J, Cerda A, Barrena R, Ponsá S, Gea T, Sánchez A. 2015. From wastes to high value added products: novel aspects of SSF in the production of enzymes. Critical reviews in environmental science and tecnology 45, 1999–2042. http://dx.doi.org/10.1080/10643389.2015.1010423

Eliasashvili V, Penninckx M, Kachlishvili E, Tsiklauri N, Metreveli E, Kharziani T, Kvesitadze G. 2008. Lentinus edodes and Pleurotus species lignocellulolytic enzymes activity in submerged and solid-state fermentation of lignocellulosic wastes of different composition. Bioresource Technology 99, 457–62. http://dx.doi.org/10.1016/j.biortech.2007.01.011

Khambhaty Y, Ananth S, Sreeram KJ, Rao JR, Nair BU. 2015. Dual utility of a novel, copper enhanced laccase from Trichoderma aureoviridae. International journal of biological Macromolecules 29(81), 69–75. http://dx.doi.org/10.1016/j.ijbiomac.2015.07.051

Kunamneni A, CamareroS, García-Burgos C, Plou FJ, Ballesteros A, Alcalde M. 2008. Engineering and Applications of fungal laccases for organic synthesis. Microbial Cell Factories 7, 32. https://doi.org/10.1186/1475-2859-7-32

Moldes D, Cadena E M, Vidal T. 2010. Biobleaching of eucalypt kraft pulp with a two laccase mediator stages sequence. Biresource Technology 101, 6924-6929. http://dx.doi.org/10.1016/j.biortech.2010.03.127.

Pandey A, Soccol CR, Nigam P, Soccol VT. 2000. Biotechnological potential of agroindustrial residues. I: Sugarcane Bagasse. Bioresource and Technology 74, 69-80. https://doi.org/10.1016/S0960-8524(99)00142-X

Ratanapongleka K, Phetsom J. 2014. Decolourization of synthetic dyes by crude laccase from Lentinus polychrous Lev. International Journal of Chemical Engineering and Applications 5(1), 1-5. http://dx.doi.org/10.7763/IJCEA.2014.V5.345

Saratale RG, Saratale GD, Chang JS, Govindwar SP. 2011. Bacterial decolorization and degradation of azodyes: a review. Journal of the Taiwan institute of Chemical Engineers 42, 138-157. https://doi.org/10.1016/j.ibiod.2006.08.006

Sarnthima R Khammuang S, Svasti J. 2009. Extracellular ligninolytic enzymes by Lentinus polychrous Lév. under solid-state fermentation of potential agro-industrial wastes and their effectiveness in decolorization of synthetic dyes. Biotechnology and Bioprocess Engineering 14, 513-522. http://dx.doi.org/10.1007/s12257-008-0262-6.

Shraddha SR, Sehgal S, Kamthania M, Kumar A. 2011. Laccase: microbial sources, production, purification, and potential biotechnological applications. Enzyme Research 21786. http://dx.doi.org/10.4061/2011/217861

Sivaranjani A, Madhan B, Barathidasan K. 2013. Decolourization of Acid Red 131 by using Shigella sp. isolated from Tannery Effluent. International Journal of Pharmaceutical and Biological Archives 3(5), 142-146.

Suwannawong P, Khammuang S, Sarnthima R.  2010.”Decolorization of rhodamine B and congo red by partial purified laccase from Lentinus polychrous Lév.,” Journal of Biochemical Technology 2, 182-186.

Yesilada O, Birhanli E, Ercan S, Ozmen N. 2014. Reactive dye decolorization ability of crude laccase enzyme from repeated batch culture of Funalia trogii. Turkish Journal of Biology 38, 103-110. http://dx.doi.org/10.3906/biy-1308-38.

Zeng X, Cai Y, Liao X, Li W, Zhang D. 2011. Decolourization of synthetic dyes by crude laccase from a newly isolated Trametes trogii strain cultivated on solid agro- industrial residue. Journal of Hazardous materials 187, 517-52. http://dx.doi.org/10.1016/j.jhazmat.2011.01.068.

Related Articles

Yield performance assessment of different mustard cultivars under field conditions

Md. Khan Jahan Ali, Md. Moshiur Rahman, Kamrun Nahar, Sharmin Ara Jannat, Mst. Khadija Khatun, Sushan Chowhan, Md. Habibur Rahman, Int. J. Biosci. 27(2), 277-282, August 2025.

Species composition of xylomicobiota of some woody plants distributed in Azerbaijan

K. F. Bakhshaliyeva, V. Y. Hasanova, N. R. Namazov, B. N. Aliyeva, S. C. Garayeva, S. E. Nagiyeva, P. Z. Muradov, Int. J. Biosci. 27(2), 267-276, August 2025.

Perception of producers and processors on sorghum diversity in the context of climate change in center and Northern Benin

Parfait Segla Alohoutade1,2,3, Alphonse Sako Avocefohoun*1,2,3, Sènan Vodouhe2, Mohamed Kanazoe1,2,3, Nicodème Chabi1, Lamine Said Baba-Moussa3, Int. J. Biosci. 27(2), 256-266, August 2025.

Implications of aberrant glycosylation on age-related disease progression

Tahmid Ahmad Patwary, Mukramur Rahman, Md. Nafis Fuad Prottoy, Sayad Md. Didarul Alam, Int. J. Biosci. 27(2), 243-255, August 2025.

Epizootic status and eradication of parasitic diseases in brown bears transferred to the rehabilitation center in Azerbaijan

Siala İ. Rustamova, Aygun A. Azizova, Gular R. Mammadova, Ramin S. Mammadov, Int. J. Biosci. 27(2), 236-242, August 2025.

Cytotoxic and apoptotic effects of Annona squamosa (Atis) crude leaf extract against A549 (Human Lung Adenocarcinoma) cell line

Ashton U. Lim, Noricel U. Garcia, Alkauzar H. Tantong, Int. J. Biosci. 27(2), 226-235, August 2025.

Perceptions of stakeholders towards sheep and goat dairy products in Benin

Yvette Adje, Philippe Sessou, Aretas Tonouhewa, Paulin Azokpota, Lamine Baba-Moussa, Souaïbou Farougou, Int. J. Biosci. 27(2), 211-225, August 2025.

Medicinal plants sold in Daloa markets: Traditional knowledge and Public health issues

Kouakou Yao Bertin, Kouakou Assoman Serge Alain, Kouame Yao Anicet Gervais, Malan Djah François, Bakayoko Adama, Int. J. Biosci. 27(2), 200-210, August 2025.