CO1 based DNA barcoding of some pentatomomorpha bugs (Hemiptera: Heteroptera) from Swat, Pakistan
Paper Details
CO1 based DNA barcoding of some pentatomomorpha bugs (Hemiptera: Heteroptera) from Swat, Pakistan
Abstract
DNA barcodes are useful for the identification of species. This study is an expansion of the database of barcodes by adding some cytochrome oxidase I (COI) gene sequences of Pentatomomorpha species of Pakistan. The COI gene sequences obtained in this study were analyzed for nucleotide composition and divergence at intraspecific, interspecific and intergeneric level. Phylogenetic trees were constructed by Neighbor Joining, Maximum Likelihood and Minimum Evolution methods using the MEGA 7 software. COI gene sequences of thirteen species were deposited to Barcode of Life Data System (BOLD). Out of the total sequences for eight species, namely, Hotea curculionoides Herrich-Schaeffer, Adria parvula Dallas, Neohalys acuticornis Ahmad and Perveen, Scotinophara ochracea Distant, Lachnesthus singalensis Dohrn, Rhyparothesus dudgeoni Distant, Neophysopelta schlanbuschi Ahmad & Abbas, Homoeocerus sigilatus Stal this is the first molecular study which has generated distinct barcodes for each species. Analysis of the COI gene sequences shows that barcode gap was distinct for between species, genera and at higher taxonomic levels. An increase in the mean K2P divergence across different taxonomic levels was observed. The mean intraspecific divergence was (0.7%). The mean interspecific divergence was (11.7%) and the mean divergence between genera was (21.1%). The mean A+T contents were (66.3%). From the Neighbor Joining, Maximum Likelihood and Minimum Evolution clustering analyses, we concluded that DNA Barcoding is quite helpful for identification of Pentatomomorpha species of the area. This study encourages using DNA barcodes as complement with a morphological taxonomy for easy identification of plant bug species.
Ball SL, Hebert PDN. 2005. Biological identifications of mayflies (Ephemeroptera) using DNA barcodes. Journal of the North American Benthological Society 24, 508-524. http://dx.doi/abs/10.1899/04-142.1
Barman AK, Joyce AL, Torres R, Higbee BS. 2017. Assessing Genetic Diversity in Four Stink Bug Species, Chinavia hilaris, Chlorochroa uhleri, Chlorochroa sayi, and Thyanta pallidovirens (Hemiptera: Pentatomidae), Using DNA Barcodes. Journal of Economic Entomology 110, 2590–2598. http://dx.doi.org/10.1093/jee/tox227
Baskar A, Dhanasekaran S, Tirumurugaan KG, Vignesh AR, Prakash B. 2014. Molecular Genetic Variation and Phylogeny of Genus Rhynocoris Based on mitochondrial Cytochrome c Oxidase Subunit I Gene (Heteroptera: Reduviidae). Indian Journal of Natural Science 4, 1474-1484.
Clare EL, Lim BK, Engstrom MD, Eger JL, Hebert PN. 2007. DNA barcoding of Neotropical bats: species identification and discovery within Guyana. Molecular Ecology Notes 7, 184-190. http://dx.doi.org/10.1111/j.1471-8286.2006.01657.x
Costa FO, Carvalho GR. 2007. The Barcode of Life Initiative: synopsis and prospective societal impacts of DNA barcoding of Fish. Genomics, Society and Policy 3, 52-56. http://dx.doi.org/10.1186/1746-5354-3-2-29
Felsenstein J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39, 783-791. http://dx.doi.org/10.1111/j.15585646.1985.tb00420.x
Frezal L, Leblois R. 2008. Four years of DNA barcoding: current advances and prospects. Infection, Genetics and Evolution 8, 727-736. http://dx.doi.org/10.1016/j.meegid.2008.05.005.
Habeeb SKM, Sanjayan KP. 2011. Sequencing and phylogenetic analysis of the mitochondrial cytochrome c oxidase subunit i of Oxycarenus laetus (Hemiptera: Lygaeidae). International Journal of Plant, Animal and Environmental Science 1, 85-92.
Hanner R, Schindel D, Ward B, Hebert P. 2005. Fish Barcode of Life (FISH-BOL). Workshop report University of Guelph, Ontario Canada, 5-8 June 2005. p 21.
Hebert PDN, Cywinska A, Ball SL, Waard JD. 2003. Biological identifications through DNA barcodes. Proceedings of Royal Society, London, B. 270, 313–321. http://dx.doi.org/10.1098/rspb.2002.2218
Hebert PDN, Penton EH, Burns JM, Janzen DH, Hallwachs W. 2004a. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences USA 101, 14812-14817. http://dx.doi.org/10.1073/pnas.0406166101
Hebert PDN, Stoeckle MY, Zemlak TS, Francis CM. 2004b. Identification of birds through DNA barcodes. PLoS Biology 2, 1657-1663. http://dx.doi.org/10.1371/journal.pbio.0020312
Hogg ID, Hebert PDN. 2004. Biological identification of springtails (Collembola: Hexapoda) from the Canadian Arctic, using mitochondrial DNA barcodes. Canadian Journal of Zoology 82, 749-754. http://dx.doi.org/10.1139/z04-041
Jung S, Duwal RK, Lee S. 2011. COI barcoding of true bugs (Insecta, Heteroptera). Molecular Ecology Resources 11, 266–270. http://dx.doi.org/10.1111/j.1755-0998.2010.02945.x.
Kaur H, Sharma K. 2017. COI-based DNA barcoding of some species of Pentatomidae from North India (Hemiptera: Heteroptera). Mitochondrial DNA A. 28(5), 756-761. http://doi.org/10.1080/24701394.2016.1180513
Kumar S, Stecher G Tamura K. 2016. MEGA 7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology Evolution 33, 1870-1874. http://dx.doi.org/10.1093/molbev/msw054.
Li P. 2008. Molecular Phylogenetic Research of Partial Species of Coreidae (Hemiptera: Heteroptera). Shaanxi Normal University.
Michael JR, Lars H, Stefan MK, Fabian D, Jerome M, Martin MG. 2014. Building-Up of a DNA Barcode Library for True Bugs (Insecta: Hemiptera: Heteroptera) of Germany Reveals Taxonomic Uncertainties and Surprises. Plos One 9, 1-13. http://doi.org/10.1371/journal.pone.0106940.
Ratnasingahm S, Hebert PDN. 2007. BOLD: The barcode of life data system www.barcodelife.org). Molecular Ecology 7, 355–364. http://dx.doi.org/10.1111/j.1471-8286.2007.01678.x
Raupach MJ, Hendrich L, Kuchler SM, Deister F, Moriniere J, Gossner MM. 2014. Building-up of a DNA barcode library for true bugs (Insecta: Hemiptera: Heteroptera) of Germany reveals taxonomic uncertainties and surprises. PLoS One. 9, e106940. http://dx.doi.org/10.1371/journal.pone.0106940.
Saitou N, Nei M. 1987. The neighbor-joining method. A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. 4(4), 406-425. http://dx.doi.org/10.1093/oxfordjournals.molbev.a040454
Schuh RT, Slater JA. 1995. True Bugs of the World (Hemiptera: Heteroptera). Classification and Natural History. Ithaca, Cornell University Press, 1-336.
Tembe S, Shouche Y, Ghate HV. 2014. DNA barcoding of Pentatomomorpha bugs (Hemiptera: Heteroptera) from Western Ghats of India. Meta Gene 2, 737–745. http://dx.doi.org/10.1016/j.mgene.2014.09.006.
Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PDN. 2005. DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society. B 360, 1847-1857. http://dx.doi.org/10.1098/rstb.2005.1716
Xia DJ, Zheng ZM. 2004. Discussion on Phylogenetic relationships of partial Pentatomidae insects based on sequences of cytochrome b gene. Zoological Research 25, 397-402.
Zhang Q, Yuan M, Shen Y. 2013. The complete mitochondrial genome of Dolycoris baccarum (Insecta: Hemiptera: idae). Mitochondrial DNA. 24, 469–471. http://dx.doi.org/10.3109/19401736.2013.766182.
Sana Ullah, Habib Ahmad, Muhammad Ather Rafi, 2018. CO1 based DNA barcoding of some pentatomomorpha bugs (Hemiptera: Heteroptera) from Swat, Pakistan. Int. J. Biosci., 13(4), 139-148.
Copyright © 2018 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.