In vitro anthelmintic efficacy of betel leaf (Piper betle) ethanolic extract against Ascaridia galli at varying concentrations
Paper Details
In vitro anthelmintic efficacy of betel leaf (Piper betle) ethanolic extract against Ascaridia galli at varying concentrations
Abstract
The rising incidence of anthelmintic resistance in chicken parasites has necessitated the urgent development of sustainable, environmentally acceptable alternatives to traditional synthetic medications. Ethnoveterinary medicine presents a viable approach by employing bioactive plant extracts that proficiently control helminth infections while reducing chemical residues. This study assessed the in vitro anthelmintic efficacy of Betel Leaf (Piper betle) Ethanolic Extract (BLEE) against Ascaridia galli to combat the rising anthelmintic resistance in chicken. Adult nematodes were subjected to a completely randomized design (CRD), with exposure to BLEE doses of 40, 80, and 120 mg/ml, alongside a Levamisole control. The results indicated a substantial response that was dependent on both dosage and duration. At 2 hours, 120 mg/ml BLEE resulted in 83.33% mortality, demonstrating no significant difference from Levamisole. After 6 hours, all BLEE treatments had cumulative fatality rates ranging from 90.00% to 96.67%. Phytochemical analysis revealed that tannins and saponins are the principal bioactive chemicals responsible for inducing parasite paralysis. The research suggests that P. betle extract at 120 mg/ml serves as a powerful botanical substitute for synthetic anthelmintics, endorsing its incorporation into sustainable poultry health management practices.
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Elvin P. Dalida*, Mc Nelly V. Saraña, Zandro O. Perez, 2026. In vitro anthelmintic efficacy of betel leaf (Piper betle) ethanolic extract against Ascaridia galli at varying concentrations. Int. J. Biosci., 28(4), 17-22.
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