Characterization and identification of soft rot bacterial pathogens of different fruits in Bangladesh

Paper Details

Research Paper 01/07/2016
Views (748)
current_issue_feature_image
publication_file

Characterization and identification of soft rot bacterial pathogens of different fruits in Bangladesh

Raihan Mujib Himel, Abu Ashraf Khan, Abdul Mannan Akanda, Meftahul Karim
Int. J. Biosci. 9(1), 1-9, July 2016.
Copyright Statement: Copyright 2016; The Author(s).
License: CC BY-NC 4.0

Abstract

The aim of the study was to characterize and identify the soft rot bacterial pathogens of different fruits in Bangladesh. Soft rotted fruit samples of mango, apple, banana, papaya and pineapple were collected from different areas of Bangladesh based on characteristic soft rot symptoms. From these samples, 50 isolates were isolated. Among the 50 isolates, only 17 isolates showed positive result in potato soft rot test. Among 17 potato soft rot positive bacterial isolates, 10 isolates were found as oxidative fermentative test (OF) positive. Biochemical and physiological tests were performed for characterization of 10 ‘OF’ positive and 07 OF negative bacterial isolates. Eight ‘OF’ positive isolates namely Mango 01, Mango 10, Mango 13, Mango 16, Apple 03, Banana 01, Pineapple 01 and Papaya 01 were identified as Erwinia carotovora subsp. carotovora. Remaining two ‘OF’ positive isolates namely Mango 11 and Mango 12 were identified as Dickeya dadantii (formerly Erwinia chrysanthemi). The 07 ‘OF’ negative bacterial isolates, namely Mango 02, Mango 03, Mango 05, Mango 08, Apple 01, Apple 02 and Pineapple 02 were identified as Pseudomonas marginalis.

Agrios GN. 1997. Control of plant diseases. Plant Pathology, 4th edn. California Academic Press, U.S.A pp. 200-216.

Alam SMK, Tigashi J, Ohtomo T, Namai T. 1999. Bacterial soft rot, a new disease of Pak- Choy (Brassica campestris L. chinensis group) caused by Erwinia carotovora. subsp. carotovora. Annals of the Phytopathological Society of Japan 65(1), 46-48.

Anonymous. 2009. The importance of fruit nutrition. http://www.3fatchicks.com/the-importance of-fruit-nutrition/ (Accessed on 24 February, 2014).

Anonymous. 2013. Bacterial soft rot. http://en.wikipedia.org/wiki/Bacterial_soft_rot (Accessed on 10 March, 2014).

Ayers SH, Rupp P, Johnson WT. 1919. A study of the alkali forming bacteria in milk. United States Department of Agriculture. Bulletin no. 782.

Cole M. 2008. Bacterial rotting of apple fruit. Annals of Applied Biology 47(3), 601-611. DOI: 10.1111 /j.1744-7348.1959.tb07292.x.

Dickey RS. 1979. Erwinia chrysanthemi: a comparative study of phenotypic properties of strains from several hosts and other Erwinia species. Phytopathology 69, 324-329. DOI: 10.1094/Phyto-69-324.

Dowson WJ. 1957. Plant diseases due to Bacteria, 2nd edn., Cambridge University Press, London pp. 169-177.

Dye DW. 1968. A taxonomic study of the genus Erwinia. The amylovora group. New Zealand Journal of Science 11, 590-607.

Eckert JW, Ogawa JM. 1985. The chemical control of postharvest diseases: subtropical and tropical fruits. Annual Review of Phytopathology 23, 421-454. DOI: 10.1146/annurev.py.23.090185.002225.

Gardan L, Gouy C, Christen R, Samson R. 2003. Elevation of three subspecies of Pectobacterium carotovorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov. International Journal of Systematic and Evolutionary Microbiology 53, 381-391. DOI: 10.1099/ijs.0.02423-0.

Gupta SK, Thind TS. 2006. Diseases of cruciferous vegetables. Scientific Publishers, India pp. 170-185.

Guzman M, Wang A. 1998. Symptoms description, identification of causal agent and inoculum source determination of the finger soft rot in banana. CORBANA 23 (50), 109-124.

Hopper PE, Kelman A. 1969. Bacterial top and stalk rot disease of corn in Wisconsin. Plant Disease Reporter 53, 66-70.

Hugh R, Leifson E. 1953. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram-negative bacteria. Journal of Bacteriology 66(1), 24-26.

Khan AA, Furuya N, Ura H, Matsuyama N. 2000. Rapid identification of Erwinia Chrysanthemi isolated from soft rotted eggplant and Phalaenopsis sp. by lipid and fatty acid profiling. Journal of the Faculty of Agriculture, Kyushu University, Japan 44(3-4), 257-263.

Kim YK, Lee SD, Choi CS, Lee SB, Lee SY. 2002. Soft rot of onion bulbs caused by Pseudomonas marginalis under low temperature storage. The Plant Pathology Journal 18(4), 199-203. DOI: 10.5423/PPJ.2002.18.4.199.

Kreigh NR, Holt JG EDs. 1984. Burgey’s manual of systematic bacteriology. Vol. I Williams and Wilkins, London pp. 141-177.

Lelliot RA, Billing E, Hayward EC. 1966. A determinative scheme for the fluorescent plant pathogenic Pseudomonads. J. App. Bacteriol 29, 470-489. DOI: 10.1111/j.1365-2672.1966.tb03499.x.

Lelliot RA, Dickey RS. 1974. Genus VII. Erwinia. Winslow, Broadhurst, Buchanan, Krumwiede, Rogers and Smith 1920, 209AL, Pp. 469- 476. In: Krieg, N. R. and J. G. Holt (eds), Bergey’s Manual of Systematic Bacteriology Vol. 1. Williams & Wilkins Co, Baltimore.

Miaruddin M, Shahjahan M. 2008. Post-harvest technology of fruits and vegetables. In: Agricultural Technology Manual. Bangladesh Agricultural Research Institute, Joydebpur, Gazipur pp. 121-130.

Mortensen CN. 1997. Seed bacteriology laboratory guide. Danish Govt. Ins. Seed pathology (DGISP) for developing countries, Copenhagen, Denmark pp. 1-2.

Pannhwar F. 2006. Post-harvest technology of fruits and vegetables (website: www.eco-web.com/edi/060529.html).

Perombelon MCM & Kelman A. 1980. Ecology of soft rot Erwinias. Annual Review of Phytopathology 18, 361-387. DOI: 10.1146/annurev.py.18. 090180.002045.

Schaad NW. 1988. Laboratory guide for identification of plant pathogenic bacteria. 2nd edition, American Phytopathological Society 164 pp.

Singh RS. 2001. Plant Disease. Oxford and IBA publishing company pvt. ltd. New Delhi pp. 86-90.

Sundararaj JS, Muthuswamy S, Palaniswami A. 1972. Bacterial rot of stored mangoes. Acta Horticulturae (ISHS) 24, 219-222.

Suslow TV, Schroth MN, Isaka M. 1982. Application of a rapid method for gram differentiation of plant pathogenic and saprophytic bacteria without staining. Phytopathology 72, 917-918. DOI: 10.1094/Phyto-72-917.

Related Articles

Muscle type and meat quality of local chickens according to preslaughter transport conditions and sex in Benin

Assouan Gabriel Bonou*, Finagnon Josée Bernice Houéssionon, Kocou Aimé Edenakpo, Serge Gbênagnon Ahounou, Chakirath Folakè Arikè Salifou, Issaka Abdou Karim Youssao, Int. J. Biosci. 27(6), 241-250, December 2025.

Effects of micronutrients and timing of application on the agronomic and yield characteristics of cucumber (Cucumis sativus)

Princess Anne C. Lagcao, Marissa C. Hitalia*, Int. J. Biosci. 27(6), 214-240, December 2025.

Response of different soybean varieties to phosphorus fertilizer microdosing and rhizobium inoculation in the sub-humid zone of Northern Benin

Pierre G. Tovihoudji*, Kamarou-Dine Seydou, Lionel Zadji, Sissou Zakari, Valerien A. Zinsou, Int. J. Biosci. 27(6), 201-213, December 2025.

On-farm validation of black soldier fly larvae meal as a sustainable replacement for shrimp meal in rainbow trout diets in the mid hills of Nepal

Ishori Singh Mahato, Krishna Paudel*, Sunita Chand, Anshuka Bhattarai, Int. J. Biosci. 27(6), 189-200, December 2025.

Insect fauna associated with Cucumis sativus (Cucurbitales: Cucurbitaceae) in Parakou, A cotton-growing area of central Benin

Lionel Zadji*, Mohamed Yaya, Roland Bocco, Prudencia M. Tovignahoua, Abdou-Abou-Bakari Lassissi, Raphael Okounou Toko, Hugues Baimey, Leonard Afouda, Int. J. Biosci. 27(6), 175-188, December 2025.

First record of two hymenopteran species, Brachymeria excarinata Gahan (Chalcididae) and Pteromalus sp. (Pteromalidae), as hyperparasitoids of Diadegma insulare in Senegal

Babacar Labou*, Etienne Tendeng, Mamadou Diatte, El hadji Sérigne Sylla, Karamoko Diarra, Int. J. Biosci. 27(6), 167-174, December 2025.

Hepatoprotective and antinociceptive effects of terpinolene in streptozotocin-induced diabetic peripheral neuropathic rats

Ravishankar Sarumathi, Muthukumaran Preethi, Chandrasekaran Sankaranarayanan*, Int. J. Biosci. 27(6), 156-166, December 2025.