home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

PCR based detection of mecA gene of Staphylococcus aureus isolated from surgical wound patients in Quetta

Paper Details

Research Paper 01/03/2022
Views (429) Download (29)
current_issue_feature_image
publication_file

PCR based detection of mecA gene of Staphylococcus aureus isolated from surgical wound patients in Quetta

Asima Safdar, Mumtaz Ali Sanjrani, Zafar Ahmed, Muhammad Alam Mengal, Fatima Abdul Majeed, Saima Azam, Fahmeeda Rehman
Int. J. Biosci.20( 3), 211-217, March 2022.
Certificate: IJB 2022 [Generate Certificate]
Key: Antibiotic susceptibilitymec-AMRSAPCR

Abstract

Staphylococcus aureus is an acquired hospital infection and which has been shown to develop resistance against several antibiotics. Polymerase Chain Reaction (PCR) was performed to categorize bacterial antibiotic-resistant. The present study aimed to examine S. aureus in pus samples through using Polymerase Chain Reaction and study the resistance of the bacterial isolates against certain antibiotics. A total of (n=100) pus samples were obtained from surgical wound patients through sterile cotton swabs.  The samples were immediately cultured, and positive samples were tested for gram staining, biochemical test, and antibiotic susceptibility. Our study showed that 58(58%) of the surgical patients were found positive for S.aureus and 42 (42%) were showed negative. This current study represents the S.aureus bimolecular identification and screening test of strain methicillin-resistant bacteria from the pus of surgical wounds of different hospitals from Quetta Balochistan. From hundred samples of 58 isolates of Staphylococcus aureus were confirmed through biochemical and PCR-based techniques. This current study reveals the S.aureus resistance variability against different antibiotics.

VIEWS 39
Cover PDF

Al–Alak SK, Kadhim Qassim D. 2016. Molecular identification of 16rRNA gene in Staphylococcus aureus Isolated from Wounds and Burns by PCR Technique and Study Resistance of Fusidic acid. Iraqi Journal of Cancer and Medical Genetics 9(1), 25-30.

Ali R, Al-Achkar K, Al-Mariri A, Safi M. 2014. Role of Polymerase Chain Reaction (PCR) in the detection of antibiotic-resistant Staphylococcus aureus. Egyptian Journal of Medical Human Genetics 15(3), 293-298.

Andre Z, Carlos C, Thierry V. 2008. Penicillin-binding proteins and b-lactam resistance. FEMS Microbiol Rev 32, 361-385.

Anupurba S, Sen MR, Nath G, Sharma BM, Gulati AK, Mohapatra TM. 2003. Prevalence of methicillin resistant Staphylococcus aureus in a tertiary referral hospital in eastern Uttar Pradesh. Indian journal of medical microbiology 21(1), 49.

Bakeet AM, Darwish SF. 2014. Phenotypic and genotypic detection of methicillin-resistant staphylococcus aureus (mrsa) in broiler chickens. Assiut Veterinary Medical Journal 60(143), 142-151.

Bannerman TL. 2008. Staphylococcus,Micrococcus and other catalase positive cocci that grow aerobically.Chapter 28. Manual of Clinical Microbiology 3384.

Bissong MEA, Wirgham T, Enekegbe MA, Niba PTN, Foka FET. 2016. Prevalence and antibiotic susceptibility patterns of methicillin resistant Staphylococcus aureus in patients attending the Laquintinie Hospital Douala, Cameroon. European Journal of Clinical and Biomedical Sciences 2(6), 92-96.

Biswas S, Karmakar A, Ghosh C. 2015. Multidrug resistant pathogenic Staphylococcus aureus in the pimples. Medical Science 16, 41-50.

Borriello SP, Murray PR, Funke G. 2005. Topley & Wilson’s Microbiology & Microbial Infections Bacteriology. Hodder Arnold.

Humaryanto H, Hanina H, Lipinwati L, Simanjuntak CA. 2020. Identification of Sccmec Type in Isolate of Methicillin Resistant Staphylococcus aureus (MRSA) In Jambi By Using Polymerase Chain Reaction (PCR). Indonesian Journal of from Tropical and Infectious Disease 8(2), 77-82.

Ito T, Katayama Y, Asada K, Mori N, Tsutsumimoto K, Tiensasitorn C,  Hiramatsu K. 2001. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrobial agents and chemotherapy 45(5), 1323-1336.

Jevons MP. 1961. “Celbenin”-resistant Staphylococci. British medical journal 1(5219), 124.

Jonas D, Speck M, Daschner FD, Grundmann H. 2002. Rapid PCR-based identification of methicillin-resistant Staphylococcus aureus from screening swabs. Journal of Clinical Microbiology 40(5), 1821-1823.

Karthy ES, Ranjitha P, Mohankumar A. 2009. Antimicrobial potential of plant seed extracts against multidrug resistant methicillin resistant Staphylococcus aureus (MDR-MRSA). International Journal of Biology 1(1), 34.

Katayama Y, Ito T, Hiramatsu K. 2000.  A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrobial agents and chemotherapy 44(6), 1549-1555.

Sajith Khan AK, Shetty PJ, Lakshmi Sarayu Y, Chidambaram A, Ranganathan R. 2012. Detection of mecA genes of methicillin-resistant Staphylococcus aureus by polymerase chain reaction. International Journal of Health and Rehabilitation Sciences 1(2), 64-8.

Shopsin B, Kreiswirth BN. 2001. Molecular epidemiology of methicillin-resistant Staphylococcus aureus. Emerging infectious diseases 7(2), 323.

Šiširak M, Zvizdić A, Hukić M. 2010. Methicillin-resistant Staphylococcus aureus (MRSA) as a cause of nosocomial wound infections. Bosnian journal of basic medical sciences 10(1), 32.

Timothi FJ. 2004. The Staphylococcus aureus “superbug”. The Journal of Clinical Investigation 1(14), 1693-96.

Wayne PA. 2002. National committee for clinical laboratory standards. Performance standards for antimicrobial disc susceptibility testing 12, 01-53.

Wertheim HF, Melles DC, Vos MC, van Leeuwen W, Van Belkum A, Verbrugh HA, Nouwen JL. 2005. The role of nasal carriage in Staphylococcusaureus infections. Lancet Infect Dis 5, 75-76.

Submit Manuscript