Influence of lactation stage and fermentation environment on Kefir yield from cow’s milk

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Research Paper 23/06/2026
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Influence of lactation stage and fermentation environment on Kefir yield from cow’s milk

Imelda Hebron*, Jilrosh Eugenio, Rosalina Sagocsoc
Int. J. Biosci. 28(6), 200-207, June 2026.
Copyright Statement: Copyright 2026; The Author(s).
License: CC BY-NC 4.0

Abstract

Kefir is a value-added fermented dairy product that can improve the profitability and sustainability of small-scale dairy farms by converting fresh milk into a nutritious product with an extended shelf life. However, limited information is available on how milk obtained at different lactation stages and simple fermentation environments influence kefir production under farm-level conditions. This study evaluated the effects of lactation stage and fermentation environment on the physicochemical properties of cow’s milk and kefir yield. A randomized complete block design was employed using nine treatment combinations consisting of three lactation stages (early, mid, and late) and three fermentation environments (thick cloth cover, closed shelf, and warmed incubator), each replicated three times. Milk was analyzed for fat, solids-not-fat (SNF), protein, and lactose, while whey volume, kefir milk volume, and kefir grain weight were determined after fermentation. Milk composition differed significantly among treatments (p<0.01). The highest fat (6.67%), protein (3.01%), and lactose (4.74%) contents were obtained from mid-lactation milk fermented in a warmed incubator, whereas the highest SNF content (7.96%) was recorded in early-lactation milk. Whey volume was significantly affected (p<0.05), with the greatest yield (119.34 mL) obtained from early-lactation milk fermented in a warmed incubator and the lowest (95.82 mL) from late-lactation milk fermented under a thick cloth cover. In contrast, kefir milk volume (221.88–240.03 mL) and kefir grain weight (6.91–8.21 g) did not differ significantly among treatments. The findings demonstrate that milk composition varies with lactation stage, while a warmed fermentation environment enhances whey production without adversely affecting kefir yield or grain growth. These results support the use of mid-lactation milk and controlled warm fermentation to optimize kefir production in small-scale dairy systems.

Abadl MMT, Marzian AA, Sulaiman R, Abas F, Hussin ASM. 2023. Optimization of coconut milk kefir beverage by RSM and screening of its metabolites and peptides. Fermentation 9(5), 430.

Bensmira M, Nsabimana C, Jiang B. 2010. Effects of fermentation conditions and homogenization pressure on the rheological properties of kefir. Food Science and Technology 43, 1180–1184.

Costa A, Lopez-Villalobos N, Sneddon N. 2019. Invited review: Milk lactose—Current status and future challenges in dairy cattle. Journal of Dairy Science 102, 5883–5898.

Gellrich K, Meyer HHD, Wiedemann S. 2014. Composition of major proteins in cow milk differing in mean protein concentration during the first 155 days of lactation and the influence of season as well as short-term restricted feeding in early and mid-lactation. Czech Journal of Animal Science 59(3), 97–106. DOI: 10.17221/7289-CJAS

Guan Y, Cui Y, Qu X, Li B, Zhang L. 2025. Post-acidification of fermented milk and its molecular regulatory mechanism. International Journal of Food Microbiology 426, 110920. DOI: 10.1016/j.ijfoodmicro.2024.110920

Hayes E, Wallace D, O’Donnell C, Greene D, Hennessy D, O’Shea N, Tobin J, Fenelon M. 2023. Trend analysis and prediction of seasonal changes in milk composition from a pasture-based dairy research herd. Journal of Dairy Science 106, 2326–2337. DOI: 10.3168/jds.2015-10740

Kurniawan M, Milanda T, Kusuma SAF. 2026. Kefir as a functional probiotic: Microbial composition and health effects. Frontiers in Food Science and Technology 5, 1725280. DOI: 10.3389/frfst.2025.1725280

Lammers BP, Heinrichs AJ, Kensinger RS. 2000. The effect of stage of lactation and milk yield on raw milk composition and quality. Journal of Dairy Science 83(3), 620–626.

Lock AL, Preseault C, Rico J, DeLand K, Allen MT. 2013. Feeding a C16:0-enriched fat supplement increased the yield of milk fat and improved conversion of feed to milk. Journal of Dairy Science 96(10), 6650–6659. DOI: 10.3168/jds.2013-6892

McGovern C, González-Orozco B, Jiménez-Flores R. 2024. Evaluation of kefir grain microbiota, grain viability, and bioactivity from fermenting dairy processing by-products. Journal of Dairy Science 107, 4259–4276.

National Research Council (US) Committee on Technological Options to Improve the Nutritional Attributes of Animal Products. 1988. Designing Foods: Animal Product Options in the Marketplace. Washington, DC: National Academies Press. Chapter: Factors Affecting the Composition of Milk from Dairy Cows.

Ostersen S, Foldager J, Hermansen JE. 1997. Effects of stage of lactation, milk protein genotype and body condition at calving on protein composition and renneting properties of bovine milk. Journal of Dairy Research 64(2), 207–219. DOI: 10.1017/S0022029996002099

Philippine Carabao Center (PCC). 2026. Buffalo milk: The udder cola. Retrieved July 16, 2026, from https://www.pcc.gov.ph/buffalo-milk-the-udder-cola-3/

Rosa DD, Dias MMS, Grześkowiak ŁM, Reis SA, Conceição LL, Peluzio MDCG. 2017. Milk kefir: Nutritional, microbiological and health benefits. Nutrition Research Reviews 30(1), 82–96. DOI: 10.1017/S0954422416000275

Wilms JN, Hare KS, Fischer-Tlustos AJ, Vahmani P, Dugan MER, Leal LN, Steele MA.  2022. Fatty acid profile characterization in colostrum, transition milk, and mature milk of primi- and multiparous cows during the first week of lactation. Journal of Dairy Science 105, 4692–4710. DOI: 10.3168/jds.2021-20880

Zilstra RT, Whittington FM, Schrama JW, Beynen AC. 2016. Casein content and casein to total protein ratio in the milk of dairy cows: A meta-analysis. Journal of Dairy Science 79(11), 2052–2061.

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