Estimation of genetic diversity in Cupressus sempervirens growing in different ecological zones of Malakand Division, KP, Pakistan
Paper Details
Estimation of genetic diversity in Cupressus sempervirens growing in different ecological zones of Malakand Division, KP, Pakistan
Abstract
The nature and magnitude of geographic genetic diversity among 11 genotypes of Pakistani Cupressussempervirens was studied through Sodium DodecylSulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE).Five populations representing different ecological conditions were sampled. A total of five loci (bands) were detected in the collected germplasm of cupresses sempervirens genotypes. Only one locus (locus-1) was found monomorphic while the remaining were polymorphic loci. Out of polymorphic loci, locus-5 showed a high degree of intra-specific locus variation (ISLV) i.e. 54.54% followed by Locus-3 which indicated 36.36%ISLVin the collected germplasm of C. sempervirence genotypes. Intra specie locus contribution towards genetic dis-agreement (LCTGD) was 80%. It was found that the genotypes of each zone occupied separate cluster in the dendrogram.PCA was carried out to complement the cluster analysis information.SDS-PAGE profiling based on two-way cluster plotting successfully resolved the genotypes on the basis of zone distribution. For better understanding of the genetic structure of C. sempervirencefurther analysis using dominant molecular markers are recommended.
Alvarez JB, Diez CM, Cuvas MA, Lopez CS, Martin LM. 2003. Cotyledon storage proteins as markers of the genetic diversity in Castanea sativa Miller. Theoratical and applied journal of genetics. 107, 730-735.
Ayten C, Leyla A, Zeki A. 2009. Biosystematics studies among EbenusL. species based on morphological, RAPD-PCR and seed protein analysis in Turkey. Pakistan journal of botany 41, 2477-2486. https://www.researchgate.net/publication/22862344 3
Badr A, El-Shazly HH, Abou EMM. 2000. Seed protein diversity and its implications on the relationships in the genus LathyrusL. (Fabaceae). In: Proc. 1st International Conference Biological Sciences, Tanta University, 333-346.
Badr A. 1995. Electrophoretic studied of seed proteins in relation to chromosomal criteria and the relationships of some taxa of Trifolium. Taxonomy44, 183-191.
Bartel JA, Adams RP, James SA, Mumba LE, Pandey RN. 2003. Variation among Cupressus species from the western hemisphere based on random amplified polymorphic DNAs. BiochemSystEcol 31. 693–702 http://dx.doi.org/10.1016/S0305-1978(02)00229-6
Bechir A, Mousadik A, Pichot C. 2004. Allozyme diversity of natural and introduced cypress stands in Morocco: consequences for management of genetic resources. Ann ForSci 61, 669–676. http://dx.doi.org/10.1051/forest:2004059
El-Kassaby YA. 2000. Effect of forest tree domestication on gene pools. In: Young A, Boshier D, Boyle T, editors. Forest Conservation Genetics: Principles and Practice. Collingwood,Victoria, Australia: Commonwealth Scientific and Industrial Research Organization Publishing 197–213 P. www.cabdirect.org/abstracts/20023103618
Ghafoor A. Arshad M. 2008. Seed protein profiling of Pisum sativum L., germplasm using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) for investigation of biodiversity. Pakistan journal of botany 40, 2315-2321.
Kamel EA. 2005. Biochemical and molecular variations in the genus RaphanusL. based on SDS-PAGE seed proteins and isozymes patterns. Assiut University Journal of Botany 34, 95-113.
Korol L, Kara N, Isik K, Schiller G. 1997 Genetic differentiation among and within natural and planted Cupressussempervirens L, eastern Mediterranean populations. Silvae Genet 46, 151– 155.
Ladizinsky G, Hymowitz T. 1979. Seed protein electrophoresis in taxonomic and evolutionary studies.Theor.Applied Genetics 54, 145-151. http://dx.doi.org/10.1007/BF00263044
Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685. http://dx.doi.org/10.1038/227680a0
Nikolic Z, Vasiljevic S, Karagic D, Vujakovic M, Jovicic D, Katic S, Surlan-Momirovic G. 2010. Genetic diversity of red clover cultivars (TrifoliumpratenseL.) based on protein polymorphism. Genetika Journal 42, 249-258. http://dx.doi.org/10.2298/GENSR1002249N
Nisar M, Ghafoor A, Wadood SF, Iqbal A, Nausheen. 2016. Intra and inter specific profiling of Pakistani Quercus species growing in the hilly areas of District Dir lower Khyber Pakhtunkhwa. Pakistan journal of botany 48(1), 263-270.
Nisar M. 2008. Genetic diversity among pea (PisumsativumL).Ph.Dthesis.Quid-e-Azamuniversity, Pakistan.
Nisar M, Ghafoor A, Khan MR, Subhan M. 2009. Genetic similarity of Pakistani pea (Pisumsativum L.) germplasm with world collection using cluster analysis and Jaccard’s similarity index. Journal of Chemical Society of Pakistan 31, 138-144. http://dx.doi.org/10.1007/s10681-011-0596-6
Papageorgiou AC. Finkeldey R, Hattemer HH, Xenopoulos S. 2005. Genetic differences among autochthonous and breeding populations of common cypress (CupressussempervirensL.) in Greece. European. Journal of forest research 124, 119-124. http://dx.doi.org/10.1007/s10342-005-0063-9
Potokina E, Duncan A, Vaughan A, Eggi EE, Tomooka N. 2000.Population diversity of the Viciasativaagg.(Fabaceae) in the flora of the former USSR deduced from RAPD and seed protein analysis. Genetics Resources and Crop Evolution 47, 171-183. http://dx.doi.org/10.17485/ijst/2010/v3i5/29742
Raddi S, Sumer S. 1999. Genetic diversity in natural Cupressussempervirense L. populations in Turkey. BiochemSyst Ecol 27, 799-814.
Rawat P, Khan MF, Kumar M, Tamarkar AK, Srivastava AK, Arya KR, Maurya R. 2010. Constituents from fruits of Cupressussempervirens. Fitoterapia 81, 162-166. http://dx.doi.org/10.1016/j.fitote.2009.08.014
Rushforth K, Adams RP, Zhong M, Ma XQ, Pandey RN. 2003. Variation among Cupressus species from the eastern hemisphere based on random amplified polymorphic DNAs. BiochemSystEcol 31, 17–24 http://dx.doi.org/10.1016/S0305-1978(02)00073-X.
Shahroodian SH, Azadfar D, Soltanloo H, Ramezanpour SS. 2011. Genetic variability in natural Iranian populations of Cupressussempervirens var. horizontalis in Caspian Sea coastward assessed by SSR markers.PlantOmics Journal 4(1), 19-24.
Sultan S, Ghafoor A, Ashraf M. 2006. Geographic pattern of Diversity of cultivated lentigermplasm collected from Pakistan as assessed by seed protein assays.ActaBiologicacracoviensia series Botanica 48(1).77–84. http://dx.doi.org/10.1007/s10681-011-0596-6
Wadood SF, Hassan N, Khaliq A, Nausheen, Jan T, Ghafoor A, Khan M, Nisar M. 2016.Genetic polymorphism in Lens culinaris collected from Malakand division Khayber Pakhtunkhwa, Pakistan. Journal of Biodiversity and Environmental Sciences 8(2), 53-60.
Zecevic B, Stevanovic D, Mladenovic-Drinic S, Konstantinov K, Jakovjevic T. 2000. Proteins as genetic markers in the study of phylogenetic relations in pepper.ActaHorticulture 579, 113-116. http://dx.doi.org/10.17660/ActaHortic.2002.579.15.
Nazim Hassan, SyedFazal Wadood, Habib Ullah, Muhammad Zahoor, Murad Khan, Asif Khan, Muhammad Nisar (2016), Estimation of genetic diversity in Cupressus sempervirens growing in different ecological zones of Malakand Division, KP, Pakistan; IJB, V8, N5, May, P15-21
https://innspub.net/estimation-of-genetic-diversity-in-cupressus-sempervirens-growing-in-different-ecological-zones-of-malakand-division-kp-pakistan/
Copyright © 2016
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0