In-vitro antioxidant, anti-lipid peroxidation, and anti-inflammatory activities of isorhamnetin: Relevance to myocardial infarction
Paper Details
In-vitro antioxidant, anti-lipid peroxidation, and anti-inflammatory activities of isorhamnetin: Relevance to myocardial infarction
Abstract
Myocardial infarction is a major cardiovascular disorder characterized by excessive oxidative stress and inflammatory responses that contribute to progressive myocardial injury. Natural flavonoids have attracted considerable attention as potential cardioprotective agents because of their antioxidant and anti-inflammatory properties. The current work used complementary biochemical assays to assess isorhamnetin’s in vitro antioxidant and anti-inflammatory capabilities. Ferric reducing antioxidant power, nitric oxide scavenging, superoxide radical scavenging, phosphomolybdenum total antioxidant capacity, and the inhibition of linoleic acid oxidation assay were used to measure antioxidant activity. Assays for protein denaturation and bovine serum albumin (BSA) denaturation inhibition were used to assess anti-inflammatory efficacy. In every test, isorhamnetin demonstrated strong concentration-dependent antioxidant activity (6-30 µM). Although its activity was still lower than that of ascorbic acid, the molecule showed high ferric reducing ability, effective prevention of lipid peroxidation, improved overall antioxidant capacity, and efficient scavenging of nitric oxide and superoxide radicals. Furthermore, isorhamnetin significantly inhibited heat-induced protein and BSA denaturation in a concentration-dependent manner, indicating notable protein-stabilizing and anti-inflammatory properties when compared with the standard drug. Collectively, these findings demonstrate that isorhamnetin possesses considerable antioxidant and anti-inflammatory activities, suggesting its potential to protect against oxidative stress- and inflammation-mediated myocardial injury. Further in vivo and mechanistic investigations are warranted to validate its cardioprotective efficacy and explore its therapeutic application in the management of cardiovascular diseases.
Apak R, Ozyurek M, Guclu K, Çapanoğlu E. 2016. Antioxidant activity/capacity measurement. I. Classification, physicochemical principles, mechanisms, and electron transfer (ET)-based assays. Journal of Agricultural and Food Chemistry 64(5), 997–1027. https://doi.org/10.1021/acs.jafc.5b04739
Bhattacharjee S, Elancheran R, Dutta K, Deb PK, Devi R. 2022. Cardioprotective potential of the antioxidant-rich bioactive fraction of Garcinia pedunculata Roxb. ex Buch.-Ham. against isoproterenol-induced myocardial infarction in Wistar rats. Frontiers in Pharmacology 13. https://doi.org/10.3389/fphar.2022.1009023
Förstermann U, Sessa WC. 2012. Nitric oxide synthases: Regulation and function. European Heart Journal 33(7), 829–837. https://doi.org/10.1093/eurheartj/ehr304
Frangogiannis NG. 2014. The inflammatory response in myocardial injury, repair, and remodelling. Nature Reviews Cardiology 11(5), 255–265. https://doi.org/10.1038/nrcardio.2014.28
Gong G, Guan YY, Zhang ZL, Rahman K, Wang SJ, Zhou S, Luan X, Zhang H. 2020. Isorhamnetin: A review of pharmacological effects. Biomedicine and Pharmacotherapy 128, 110301. https://doi.org/10.1016/j.biopha.2020.110301
Halliwell B, Gutteridge JMC. 2015. Free Radicals in Biology and Medicine. Oxford University Press.
Heim KE, Tagliaferro AR, Bobilya DJ. 2002. Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships. The Journal of Nutritional Biochemistry 13(10), 572–584.
Khan R, Khan MR, Sahreen S, Ahmed M. 2012. Assessment of flavonoid contents and in vitro antioxidant activity of Launaea procumbens. Chemistry Central Journal 6(1), 43. https://doi.org/10.1186/1752-153X-6-43
Li Y, Yao J, Han C, Yang J, Chaudhry MT, Wang S, Liu H, Yin Y. 2016. Quercetin, inflammation and immunity. Nutrients 8(3), 167. https://doi.org/10.3390/nu8030167
Liu F, Ooi VEC, Chang ST. 1997. Free radical scavenging activity of mushroom polysaccharide extracts. Life Sciences 60, 763–771.
Nawaz I, Tahir A, Iqbal SM, Anjum F, Naseem M, Aslam MI. 2023. Anti-inflammatory, anti-nociceptive and anti-pyretic activities of Cenchrus ciliaris L. Journal of Ethnopharmacology 309. https://doi.org/10.1016/j.jep.2023.116332
Osawa T, Namiki M. 1985. A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves. Agricultural and Biological Chemistry 49, 3351–3352.
Pacher P, Beckman JS, Liaudet L. 2007. Nitric oxide and peroxynitrite in health and disease. Physiological Reviews 87(1), 315–424. https://doi.org/10.1152/physrev.00029.2006
Panche AN, Diwan AD, Chandra SR. 2016. Flavonoids: An overview. Journal of Nutritional Science 5, e47. https://doi.org/10.1017/jns.2016.41
Prieto P, Pineda M, Aguilar M. 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry 269(2), 337–341.
Roth GA, Mensah GA, Johnson CO, Addolorato G, Ammirati E, Baddour LM, Barengo NC, Beaton AZ, Benjamin EJ, Benziger CP, Bonny A. 2020. Global burden of cardiovascular diseases and risk factors, 1990–2019: Update from the GBD 2019 study. Journal of the American College of Cardiology 76(25), 2982–3021.
Sreejayan, Rao MNA. 1997. Nitric oxide scavenging by curcuminoids. Journal of Pharmacy and Pharmacology 49(1), 105–107. https://doi.org/10.1111/j.2042-7158.1997.tb06761.x
Vijayalakshmi M, Ruckmani K. 2016. Ferric reducing antioxidant power assay in plant extract. Bangladesh Journal of Pharmacology 11(3), 570–572. https://doi.org/10.3329/bjp.v11i3.27663
Williams LAD, O’Connor A, Latore L, Dennis O, Ringer S, Whittaker JA, Conrad J, Vogler B, Rosner H, Kraus W. 2008. The in vitro anti-denaturation effects induced by natural products and non-steroidal compounds in heat-treated (immunogenic) bovine serum albumin (BSA) is proposed as a screening assay for the detection of anti-inflammatory compounds, without the use of animals in the early stages of the drug discovery process. West Indian Medical Journal 57(4), 327–331.
Ramaraj Dheshika, Ragunathan Subashini*, 2026. In-vitro antioxidant, anti-lipid peroxidation, and anti-inflammatory activities of isorhamnetin: Relevance to myocardial infarction. Int. J. Biosci., 29(1), 180-187.
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