Immature Embryo Culture Accelerates Soybean Reproductive Phase: A Potential Biotechnology Approach for Shortening Breeding Cycle
Paper Details
Immature Embryo Culture Accelerates Soybean Reproductive Phase: A Potential Biotechnology Approach for Shortening Breeding Cycle
Abstract
The production of soybean seeds for breeding purposes is usually constrained by a long reproductive phase. One possible way to shorten soybean reproduction cycle is through immature embryo culture. This research studied the effect of pod age and phytohormone composition on the growth of soybean plantlets originated from immature embryos, and to compare potential life cycles of soybean plants generated from embryo culture and from conventional method. Two separate experiments were studied, i.e. pod age experiment (2, 3 and 4 weeks after flowering (WAF) embryos) and experiment of phytohormones indolebutyric acid (IBA) and 6-benzylaminopurine (BAP) combinations. Data were analyzed using the SAS 9.1.3 version statistical software, and treatment means were compared using Duncan’s Multiple Range Test. Data indicated that IBA gave better effects on plantlet growth, as compared to no-hormone treatment, but BAP did not provide good effect. The highest stem (28.7 mm), maximum number of root (10.1), and longest root (63.9 mm) per plantlet were obtained from embryos grown on medium supplemented with 1.5 ppm IBA (B1). Four WAF embryos were suitable explants for better soybean plantlet growth, but in most cases, they were not significantly different from 3 WAF embryos. Culture of 3 WAF embryos can potentially shorten soybean’s life cycle (for breeding purposes) by 32 days if compared to conventional propagation using mature seeds. This way could potentially help breeders to plant soybean more often in each year.
Abbas B. 2011. Basic Principles of Tissue Culture Technique. Alfabeta Publisher. Bandung, Indonesia.
Arnold SV, Sabala I, Bozhkov P, Dyachok J, Filonova L. 2002. Developmental pathway of somatic embryogenesis. Plant Cell Tissue Organ Culture 69, 233-249. http://dx.doi.org/10.1023/A:1015673200621
Bouiamrine EH, Diouri M, Halimi RE, Chillasse L. 2013. Callus growth and plant regeneration in durum wheat (Triticum durum Desf.) immature embryos under abscisic acid (ABA) treatment. International Journal of Biosciences 3(2), 87-98. http://dx.doi.org/10.12692/ijb/3.2.87-98
Bridgen MP. 1994. A review of plant embryo culture. HortScience 29(11), 1243-1245. Christianingsih R. 2008. The effect of triacontanol and gibberellin on growth and food percentage of bean. Agros 10(2), 35-42.
Clarke HJ, Wilson JG, Kuo I, Lülsdorf MM, Mallikarjuna N, Kuo J, Siddique KHM. 2006. Embryo rescue and plant regeneration in vitro of selfed chickpea (Cicer arietinum L.) and its wild annual relatives. Plant Cell, Tissue and Organ Culture 85(2), 197-204. http://dx.doi.org/10.1007/s11240-005-9071-1
Croser J, Castello MC, Edwards K. 2010. Lupin immature seed culture for generation acceleration. CLIMA report, University of Western Australia.
Ekom DCT, Abdelwahd R, Iraqi D. 2014. Immature Embryo-Derived of Two Bread Wheat (Triticum aestivum L.) Varieties Transformation Using Particle Bombardment Method. Annual Research and Review in Biology 4(24), 3904-3914. https://doi.org/10.9734/ARRB/2014/8499
Farshadfar E, Jamshidi B, Chehri M. 2014. Assessment of immature embryo culture to select for drought tolerance in bread wheat. International Journal of Biosciences 4(4), 194-203. http://dx.doi.org/10.12692/ijb/4.4.194-203
Fereol L, Chovelon V, Causse S, Michaux – Ferriere N, Kahane R. 2002. Evidence of somatic embryogenesis Process for Plant Regeneration in Garlic (Allium sativum L). Plant Cell Reports 21, 197-203. http://dx.doi.org/10.1007/s00299-002-0498-0
Gaba VP. 2005. Plant Growth Regulator. In: Trigiano RN, Gray DJ, Ed. Plant Tissue Culture and Development. CRC Press. London, 87-100.
Gamborg O, Miller R, Ojima K. 1968. Nutrient requirements suspensions cultures of soybean root cells. Experimental Cell Research 50(1), 151-158. http://dx.doi.org/10.1016/0014-4827(68)90403-5
Hakam N, Udupa SM, Gaboun F, Rabha A, Ibriz M, Iraqi D. 2014. Effect of genotypes and culture media on embryogenic callus induction and plantlet regeneration from mature embryos of durum wheat. Romanian Agricultural Research 31, 1-8.
Ilyas S. 2005. Embryo Culture Rescue In Plants. Journal of Communication Research 17.
Kapila RK, Sethi GS. 1993. Genotype and age effect on in vitro embryo rescue of bread wheat x hexaploid triticale hybrids. Plant Cell, Tissue and Organ Culture 35, 287-291.http://dx.doi.org/10.1007/BF00037283
Kaviani B, Hashemabadi D, Khodabakhsh H, Onsinejad R, Ansari MH, Haghighat N. 2015. Micropropagation of Begonia rex Putz. by 6-benzyladenine and a-naphthalene acetic acid. International Journal of Biosciences 6(5), 8-15. http://dx.doi.org/10.12692/ijb/6.5.8-15
Ko D, Kang J, Kiba T, Park J, Kojima M, Do J, Kwon M, Endler A, Song WY, Martinoia E, Sakakibara H, Kim KY, Lee Y. 2014. Arabidopsis ABCG14 is essential for the root-to-shoot translocation of cytokinin. Proceedings of the National Academy of Science (PNAS) 111(19), 7150–7155. http://dx.doi.org/10.1073/pnas.1321519111
Kosmiatin M, Mariska I. 2005. Embryo culture and chromosome doubling from crosses of green beans and black beans. Agriculture Biotechnology Journal 10(1), 24-34.
Mattjik NA. 2005. The Role of Tissue Culture in Crop Improvement. Scientific Oration Professor of IPB. Faculty of Agriculture, IPB, Bogor, Indonesia.
Muhammad K, Gul Z, Jamal Z, Ahmed M, Khan AR, Khan ZU. 2015. Effect of coconut water from different fruit maturity stages, as natural substitute for synthetic PGR in in vitro potato micropropagation. International Journal of Biosciences 6(2), 84-92. http://dx.doi.org/10.12692/ijb/6.2.84-92
Nurdin. 2013. Growth Response of Young Soybean Embryo Age on Variation of Seed pods and Layout. MSc thesis, Halu Oleo University, Kendari, Indonesia 48-54.
Nuryeni. 2014. Optimalisation of Growth Hormone Composition for Immature Embryo Culture of Soybean (Glycine max (L.) Merril). Master thesis, Graduate Study Program, University of Halu Oleo, Kendari, Indonesia.
Pertamawati. 2010. The effect of photosynthesis on growth of potato (Solanum tuberosum L.) under photoautotroph in vitro. Jurnal Sains dan Teknologi Indonesia 12(1), 31-37.
Pishbin N, Mousavi A, Kalatejari S, Shariatpanahi M, Jahromi BB. 2014. The effect of plant growth regulators and different types of explants on in vitro regeneration of sweet pepper (Capsicum annuum L.). International Journal of Biosciences 5(5), 139-146. http://dx.doi.org/10.12692/ijb/5.5.139-146
Purnamaningsih R, Lestari EG. 1998. Multiplication of shoots Intersection dribbles through in vitro culture. Germplasm Bulletin 1(5), 24-27.
Purnawati E, Hidajat JR. 1994. Characterization of Soybean Germplasm. The collection and characterization of Agriculture Germplasm. Balitbangtan, Indonesia.
Rejthar J, Viehmannova I, Cepkova PH, Fernandez E, Milella L. 2014. In vitro propagation of Drosera intermediate as influenced by cytokinins, pH, sucrose, and nutrient concentration. Emirates Journal of Food and Agriculture 26(6), 558-564. http://dx.doi.org/10.9755/ejfa.v26i6.18022
Roumet P, Morin F. 1997. Germination of immature soybean seeds to shorten reproductive cycle duration. Crop Science 37, 521-525. http://dx.doi.org/10.2135/cropsci1997.0011183X003700020035x
Santos KGB, Mariath JEA, Moço MCC, Zanettini MHB. 2006. Somatic Embryogenesis from Immature Cotyledons of Soybean (Glycine max (L.) Merr.): Ontogeny of Somatic Embryos. Brazilian Archives of Biology and Technology 49(1), 49-55. http://www.scielo.br/pdf/babt/v49n1/28487
Sharma HC, Gill BS. 1983. New hybrids between Agropyron and wheat. 2. Production, morphology and cytogenetic analysis of F hybrids and backcross derivatives. Theoretical and Applied Genetic 66, 111–121. http://dx.doi.org/10.1007/BF00265184
Shen X, Gmitter FG, Grosser JW. 2011. Immature embryo rescue and culture. Methods in Molecular Biology 710, 75-92. http://dx.doi.org/10.1007/978-1-61737-988-8_7
Short KC, Torrey JG. 1972. Cytokinin in seedling roots of pea. Plant Physiology 49, 155-160.
Slamet. 2011. Development of Acclimatization Technique for In vitro Generated Soybean Plants. Jurnal Litbang Pertanian 30(2), 48-54.
Sofia D. 2007. Effect of various concentrations of benzylamino purine and cycocel the soybean embryo growth in vitro. Faculty of Agriculture, University of North Sumatera, Indonesia.
Sungkumlong, Deb CR. 2008. Effects of different factors on immature embryo culture, PLBs differentiation and rapid mass multiplication of Coelogyne suaveolens (Lindl.) Hook. Indian Journal of Experimental Biology 46, 243-248.http://www.ncbi.nlm.nih.gov/pubmed/18512333
Tilton VR, Russel SH. 1984. In vitro culture of immature Soybean embryos. Plant Physiology 115, 191-200. http://dx.doi.org/10.1016/S0176-1617(84)80119-4
Triatminingsih, Fitrianingsih RI, Sinaga EB, Wahyu D. 2001. Effect of some level concentrations of IBA and radiation treatment on plantlet rooting mangosteen in vitro. Journal Horticulture 1(14), 232-236.
Ulfah R. 2009. Effects of IBA and BAP on shoot multiplication of turmeric (Curcuma domestica Val.) in vitro. Laboratory VEDCA, Cianjur, West Java, Indonesia.
Uzun S, Parmaksiz I, Uranbey S, Mirici S, Sarihan EO, Ipek A, Kaya MD, Gurbuz B, Arslan N, Sancak C, Khawar KM, Ozcan S. 2014a. In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik. Turkish Journal of Biology 38, 83-88. http://dx.doi.org/10.3906/biy-1307-52
Uzun S, Ilbas AI, Ipek A, Arslan N, Barpete S. 2014b. Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii. Turkish Journal of Agriculture and Forestry 38, 348-353.http://dx.doi.org/10.3906/tar-1306-47
Van K, Jang HJ, Jang YE, Lee SH. 2008. Regeneration of Plants from EMS-treated Immature Embryo Cultures in Soybean [Glycine max (L.) Merr.]. Journal Crop Science and Biotechnology 11(2), 119-126.
Verma K, Saini R, Rani A. 2014. Recent advances in the regeneration and genetic transformation of soybean. Journal of Innovative Biology 1(1), 15-26.
Wattimena GA, Nurhajati AM, Wiendi NMA, Efendi D, Purwoko BS, Khumaida N. 2011. Biotechnology in Plant Breeding. IPB Press, Bogor, Indonesia.
Wijayanto T, Sadimantara GR, Nurdin. 2013a. Effect of young seed position in the pod on the growth of in vitro plantlets of soybean. Agriplus 23(3), 214-218.
Wijayanto T, Sadimantara GR, Boer D, Erawan D, Nurdin, Barker SJ. 2013b. Immature embryo culture for acceleration of soybean (Glycine max L. Merrill) reproductive cycle. Proceeding of the 8th International Conference on Innovation and Collaboration towards Asean Community. Kendari, Indonesia 17-19 Oct 2013.
Wijayanto T, Sadimantara GR, Haq M. 2014. Testing of an alternative method of growth of young seedlings of soybean to shorten the cycle of breeding for the development improved variety for specific location. Maritime Seminar, Kendari, Indonesia, 22-23 March 2014.
Yan H, Lu L, Alzate A, Ceballos H, Hershey C, Chen S, Li K. 2014. Fruit, seed and embryo development of different cassava (Manihot esculenta Crantz) genotypes and embryo rescue. African Journal of Biotechnology 13(4), 1542-1528. http://dx.doi.org/10.5897/AJB2013.13180
Zulkarnain. 2009. Plant Tissue Culture Plant Propagation Solutions. Earth Literacy. Jakarta, Indonesia.
Teguh Wijayanto, Gusti Ray Sadimantara, Suaib, Norma Arif, Dirvamena Boer, Dedi Erawan, Nuryeni, Nurdin, Mubayyinul Haq (2016), Immature Embryo Culture Accelerates Soybean Reproductive Phase: A Potential Biotechnology Approach for Shortening Breeding Cycle; IJB, V9, N2, August, P37-48
https://innspub.net/immature-embryo-culture-accelerates-soybean-reproductive-phase-a-potential-biotechnology-approach-for-shortening-breeding-cycle/
Copyright © 2016
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0