Cytotoxic effects of aqueous and methanolic extracts of Incarvillea emodi (Royle Ex Lindl.) Chatterjee on mammalian cells

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Research Paper 01/08/2016
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Cytotoxic effects of aqueous and methanolic extracts of Incarvillea emodi (Royle Ex Lindl.) Chatterjee on mammalian cells

Yasir Ihtesham, Uzma Khan, Wajiha Khan, Ikram Ullah
Int. J. Biosci. 9(2), 104-109, August 2016.
Copyright Statement: Copyright 2016; The Author(s).
License: CC BY-NC 4.0

Abstract

The present study is designed to investigate the comparative cytotoxic activity of crude aqueous and methanolic extracts of different parts (aerial parts with flowers, roots and aerial parts with fruits) of Incarvillea emodi collected from different Himalayan regions in Pakistan, against CHO-K1 (Chinese hamster ovary cell line). In vitro cytotoxic assay was performed by the crystal violet assay. Cells were suspended with trypsin/EDTA in 10 % calf serum. Hemocytometer was used for cell counting. The absorbance was measured using a microplate reader at a wavelength of 562 nm and percentage growth inhibition was calculated. The crude aqueous and methanolic extract (1-200 µg/ml concentration) of Incarvillea emodi was taken for cytotoxic activity. At highest concentration i.e. 200µg/ml percentage growth inhibition was recorded above 90% for all the six samples of the plant. The results showed that cell growth is significantly lower in extract treated cells compared to untreated control. Based on the results it is determined that Incarvillea emodi is a significant source of biologically active substances that have cytotoxic activity in vitro.

Aini NAS, Merrina A, Stanslas J, Sreenamanan S. 2008. Cytotoxic potential on breast cancer cells using selected forest species found in Malaysia. International Journal of Cancer Research 4, 103-109.

Aisha AFA, Nassar ZD, Siddique MJ, Abu-Salah KM, Alrokayan SA, Ismail Z, Abdul-Majid AMS. 2011. Evaluation of antiangiogenic, cytotoxic and antioxidant effects of Syzygium aromaticum L. extract. Asian Journal of Biological Sciences 4, 282-290. DOI: 10.3923/ajbs.2011.282.290.

Anandakumar S, Karmegam N. 2011. In vitro cytotoxic evaluation of Hugonia mystax Linn. leaf and stem bark extracts. International Journal of Botany 7(4), 300-304.

Bisht VK, Negi JS, Bhandari AK, Sundriyal RC. 2011. Anticancerous plants of Uttarakhand Himalaya: A review. International Journal of Cancer Research 7, 192-208.

Ceschini L and Campos EG. 2006. Cytotoxic effects of Cochlospermum regium (Mart & Schrank) Pilger aqueous root extract on mammalian cells. Journal of Ethnopharmacology 103, 302-305. Doi: 10. 1016/j.jep.2005.07.026.

Chi YM, Hashimoto F, Yan WM, Nohara T, Yamashita M, Marubayashi N. 1997a. Monoterpene alkaloid from Incarvillea sinensis. VII. Absolute stereochemistry of incarvilline and structure of a new alkaloid, hydroxyincarvilline. Chemical and Pharma-ceutical Bulletin 45, 495-498.

Chi YM, Hashimoto F, Yan WM, Nohara T. 1995a. Two alkaloids from Incarvillea sinensis. Phytochemistry 39, 1485-1487.

Chi YM, Nakamura M, Zhao XY, Yoshizawa T, Yan WM, Hashimoto F, Kinjo J, Nohara T. 2005a. A monoterpene alkaloid from Incarvillea sinensis. Chemical and Pharmaceutical Bulletin 53, 1178-1179. Doi: 10.1016/j.jep.2013.11.027. Epub 2013 Nov 21.

Durga MSS, Satyavathi K, Bhojaraju P, Preeti PM, Lovaraju KP, Priya KS, Kumari YR, Prasad DV, Lakshmi KK. 2013. Cytotoxic and anthelmintic activities of methanolic extract of Parthenium hysterophorus L. Pharmacophore 4(6), 275-279.

Galati G and O’Brien PJ. 2004. Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radical Biology and Medicine 37, 287-303. DOI:10.1016/j.freeradbiomed.2004.04.034.

Hafidh RR, Abas F, Abdulamir AS, Jahanshiri F, Bakar FA, Sekawi Z. 2009. A review: Cancer research of natural products in Asia. International Journal of Cancer Research 5, 69-82.

Kpoviessi BGHK, Salomé DSK, Eléonore YL, Fernand G, Michel F, Mansourou M, Joëlle Q, Georges CA, Joanne B. 2014. In vitro anti-trypanosomal and antiplasmodial activities of crude extracts and essential oils of Ocimum gratissimum Linn from Benin and influence of vegetative stage. Journal of Ethnopharmacology 155, 1417–1423. Doi: 10.1016/j.jep.2014.07.014. Epub 2014 Jul 21.

Kpoviessi S, Joanne B, Pierre A, Fernand G, Bénédicta K, Brice S, Georges A, Michel F, Mansourou M, Joëlle Q. 2014. Chemical composition, cytotoxicity and in vitro antitrypanosomal and antiplasmodial activity of the essential oils of four Cymbopogon species from Benin. Journal of Ethnop-harmacology 151, 652-659.

Lu T, Zhang WD, Pei YH, Zhang C, Li JC, Shen YH. 2007. A new iridoid from Incarvillea delavayi. Chinese Chemical Letters 18, 1512-1514.

Luo Y, Yi J, Li B, Zhang G. 2004. Novel ceramides and a new glucoceramide from the roots of Incarvillea arguta. Lipids 39(9), 907-913.

Nakamura M, Chi YM, Yan WM, Nakasugi Y, Yoshizawa T, Irino N, Hashimoto F, Kinjo J, Nohara T, Sakurada S. 1999a. Strong antinoniceptive effect of incarvillateine, a novel monoterpene alkaloid from Incarvillea sinensis. Journal of Natural Product Research 62, 1293-1294.

Rafter JJ. 2002. Scientific basis of biomarkers and benefits of functional foods for reduction of disease risk: Cancer. British Journal of Nutrition 88, S219-S224.

Shier WT. 1991. Mammalian Cell Culture on $5 a Day. A Laboratory Manual of Low Cost Methods, University of the Philippines at Los Baños Publications 132 pp., ISBN: 971-547-001-7.

Siddique MJ, Ismail Z, Aisha AFA, Abdul-Majid AMS. 2010. Cytotoxic activity of Catharanthus roseus (Apocynaceae) crude extracts and pure compounds against human colorectal carcinoma cell line. International Journal of Pharmacology 6, 43-47. DOI: 10.3923/ijp.2010.43.47.

Sunilson JAJ, Rejitha G, Anandarajagopal K, Amitara D, Muthappan M, Promwichit P. 2009. Cytotoxic effect of Cayratia carnosa leaves on human breast cancer cell lines. International Journal of Cancer Research 5, 115-122.

Uddin K, Sayeed A, Islam A, Rahman AA, Ali A, Khan GRMAN, Sadik MG. 2003. Purification, characterization and cytotoxic activity of two flavonoids from Oroxylum indicum vent. (Bignoniaceae). Asian Journal of Plant Sciences 2, 515-518.

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