Antibiotic resistance pattern and gender wise prevalence of Pseudomonas aeruginosa strain isolated from the tertiary health care units
Paper Details
Antibiotic resistance pattern and gender wise prevalence of Pseudomonas aeruginosa strain isolated from the tertiary health care units
Abstract
Antibiotic resistance is a matter of great concern and it has finally attracted the attention of scientists and mass-media worldwide. The present study aims to determine the gender wise prevalence and multidrug resistance in P. aeruginosa isolated from clinical samples of public and private health care units. The 280 clinical samples (118 female and 162 male) of pus, urine, pleural fluid, blood, high vaginal swabs, throat, ear, stool, cerebrospinal fluid and ascitic fluid were collected from public and private health care hospitals of Khairpur and Sukkur cities of Pakistan. The isolates were isolated and identified using conventional microbiological methods and molecular characterization using 16S rRNA gene sequence homology. The antimicrobial sensitivity was determined using Kirby-Bauer’s disc-diffusion method according to the clinical laboratory standard institute. The Overall 28% samples were found positive for the isolation of P. aeruginosa and highest 100% prevalence was found in ascitic fluid, followed by ear swab 50% while the lowest percent prevalence was in urine (3.8%) samples. The overall prevalence was found more in male patient than female patients. The results of antibiotic sensitivity profiling revealed complete resistance against all the antibiotics tested. The phylogenetic correlation of amplified 16S rRNA gene sequence of isolate shared 99% similarity with P. aeruginosa strain (Gen Bank accession no. JF513146.1). Increasing multidrug resistance among Pseudomonas is an alarming situation in a hospital setting and requires prompt steps to cope up with resistance.
Abreu PM, Farias PG, Paiva GS, Almeida AM, Morais PV. 2014. Persistence of microbial commun-ities including Pseudomonas aeruginosa in a hospital environment: a potential health hazard. BMC Micro-biology 14, 118. DOI: 10.1186/1471-2180-14-118.
Ali Z, Mumtaz N, Naz SA, Jabeen N, Shafique M. 2015. Multi-Drug Resistant Pseudomonas Aeruginosa: A threat of nosocomial infections in tertiary care hospitals. Journal of Pakistan Medical Association 65(12).
Ansari A, Salman SM, Yaqoob S. 2015. Antibiotic Resistance Pattern in Pseudomonas aeruginosa Strains Isolated at Era s Lucknow Medical College and Hospital, Lucknow, India. International Journal of Current Microbiology and Applied Science 1, 48-58.
Barbier F, Wolff M. 2010. Multi-drug resistant Pseudomonas aeruginosa: towards a therapeutic dead end. Medicine Science 26, 960-968. DOI: 10.1051/medsci/20102611960.
Bonten MJ, Bergmans DC, Speijer H, Stobberingh EE. 1999. Characteristics of polyclonal endemicity of Pseudomonas aeruginosa colonization in intensive care units. Implications for infection control. American Journal of Respiratory and Critical Care Medicine 160, 1212-1219. doi: 10.1164/ajrccm.160.4.9809031.
Brewer SC, Wunderink RG, Jones CB, Leeper KV. 1996. Ventilator-associated pneumonia due to Pseudomonas aeruginosa. CHEST Journal109(4), 1019-1029. DOI:10.1378/chest.109.4.1019.
Cabot G, Ocampo-Sosa AA, Dominguez MA, Gago JF, Juan C, Tubau F, et al. 2012. Spanish Network for Research in Infectious Diseases (REIPI): genetic markers of widespread extensively drug-resistant Pseudomonas aeruginosa high-risk clones. Antimicrobial Agents and Chemotherapy 56, 6349-57. DOI: 10.1128/AAC.01388-12.
Carmeli Y, Troillet N, Eliopoulos GM, Samore MH. 1999. Emergence of antibiotic resistant Pseudomonas aeruginosa: comparison of risks associated with different antipseudomonal agents. Antimicrobial Agents Chemotherapy 43, 1379-82.
Chastre J, Wunderink R, Prokocimer P, Lee M, Kaniga K, Friedland I. 2008. Efficacy and safety of intravenous infusion of doripenem versus imipenem in ventilator-associated pneumonia: a multicenter, randomized study. Critical Care Medicine 36, 1089-96. DOI: 10.1097/CCM.0b013e3181691b99.
Cobos-Trigueros N, Solé M, Castro P, Torres JL, Hernández C, Rinaudo M, Martínez JA. (2015). Acquisition of Pseudomonas aeruginosa and its resistance phenotypes in critically-ill medical patients: role of colonization pressure and antibiotic exposure. Critical Care19(1), 218. DOI:10.1186/3054-015-0916-7.
Coggan KA, Wolfgang MC. 2012. Global regulatory pathways and cross-talk control Pseudomonas aeruginosa environmental lifestyle and virulence phenotype. Current Issues in Molecular Biology 14, 47-70.
El Amari EB, Chamot E, Auckenthaler R, Pechère JC, Van Delden C. 2001. Influence of previous exposure to antibiotic therapy on the susceptibility pattern of Pseudomonas aeruginosa bacteremic isolates. Clinical Infectious Disease 33, 1859-64. DOI: 10.1086/324346.
Gurjar M. 2015. Colistin for lung infection: an update. Journal of Intensive Care 3, 3. DOI 10.1186/s40560-015-0072-9.
Khan JA, Iqbal Z, Rahman SU, Farzana K, Khan A. 2008. Report: prevalence and resistance pattern of Pseudomonas aeruginosa against various antibiotics. Pakistan journal of pharmaceutical sciences 21(3), 311-315.
Kollef MH, Chastre J, Fagon JY, François B, Niederman MS, Rello J, et al. 2014.Global prospective epidemiologic and surveillance study of ventilator-associated pneumonia due to Pseudomonas aeruginosa. Critical Care Medicine 42, 2178-8. DOI: 10.1097/CCM.0000000000000510.
Lister PD, Wolter DJ, Hanson ND. 2009. Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clinical Microbiology Review 22, 582-610. DOI: 10.1128/CMR.00040-09.
Manjunath GN, Prakash R, Annam V, Shetty K. 2011. Changing trends in the spectrum of antimicrobial drug resistance pattern of uropathogens isolated from hospitals and community patients with urinary tract infections in Tumkur and Bangalore. International Journal of Biological & Medical Research 2(2), 504-507.
Micek ST, Wunderink RG, Kollef MH, Chen C, Rello J, Chastre J, Menon V. 2015. An international multicenter retrospective study of Pseudomonas aeruginosa nosocomial pneumonia: Impact of multi-drug resistance. Critical Care 19(1), 219. DOI: 10.1186/s13054-015-0926-5.
Nasreen M, Sarker A, Malek MA, Ansaruzzaman M, Rahman M. 2015. Prevalence and Resistance Pattern of Pseudomonas aeruginosa Isolated from Surface Water. Advances in Microbiology 5(01), 74.
Nseir S, Blazejewski C, Lubret R, Wallet F, Courcol R, Durocher A. 2011.Risk of acquiring multidrug-resistant Gram-negative bacilli from prior room occupants in the intensive care unit. Clinical Microbiology and Infection 17, 1201-1208. DOI: 10.1111/j.1469-0691.2010.03420.
Oikonomou O, Panopoulou M, Ikonomidis A. 2011. Investigation of carbapenem heteroresistance among different sequence types of Pseudomonas aerugin-osa clinical isolates reveals further diversity. Journal of Medical Microbiology 60, 1556-8.
Ong DSY, Jongerden IP, Buiting AG, Leverstein-van Hall MA, Speelberg B, Kesecioglu J. et al. 2011. Antibiotic exposure and resistance development in Pseudomonas aeruginosa and Enterobacter species in intensive care units. Critical Care Medicine 39, 2458-63. DOI: 10.1097/CCM.0b013e318225756d.
Parker CM, Kutsogiannis J, Muscedere J, Cook D, Dodek P, Day AG, Canadian GTCC. 2008. Ventilator-associated pneumonia caused by multidrug-resistant organisms or Pseudomonas aeruginosa: prevalence, incidence, risk factors, and outcomes. Journal of critical care 23(1), 18-26.DOI:10.1016/J.JCRC.2008.02.001.
Prakash V, Mishra PP, Premi HK, Walia A, Dhawan S, Kumar A. 2014. Increasing incidence of multidrug resistant Pseudomonas aeruginosa in inpatients of a tertiary care hospital. International Journal of Research in Medical Sciences 2, 1302-1306. DOI: 10.5455/2320-6012.ijrms20141111.
RiouM, Carbonnelle S, Avrain L, Mesaros N, Pirnay JP, Bilocq F, et al. 2010. In vivo development of antimicrobial resistance in Pseudomonas aeruginosa strains isolated from the lower respiratory tract of Intensive Care Unit patients with nosocomial pneumonia and receiving antipseudomonal therapy. International Journal of Antimicrobial Agents 36, 513-522. DOI: 10.1016/j.ijantimicag.2010.08.005.
Schillaci D, Cascioferro S. 2015. The Re-Discovering of Old Molecules to Face the Antibiotic Crisis. J. Microb. Biochem.Technol7(03). DOI:10.4172/1948-5948.1000e121.
Schillaci1 D, Cascioferro S. 2015. The Re-Discovering of Old Molecules to face the Antibiotic Crisis, Microbial & Biochemical Technology 7(3). DOI:10.4172/1948-5948.1000e121.
Sedighi M, Safiri S, Pirouzi S, Jayasinghe H, Sepidarkish, M, Fouladseresht H. 2015. Detection and Determination of the Antibiotic Resistance Patterns in Pseudomonas aeruginosa Strains Isolated from Clinical Specimens in Hospitals of Isfahan, Iran. Scimetr 3(1), e21133. DOI: 10.5812/scimetr.21133.
Venier AG, Leroyer C, Slekovec C, Talon D, Bertrand X, Parer S, et al. 2014. Risk factors for Pseudomonas aeruginosa acquisition in intensive care units: a prospective multicentre study. Journal of Hospital Infection 88, 103-108. DOI:10. 1016/j.jhin.2014.06.018.
Vincent JL, Sakr Y, Sprung CL. 2006. Sepsis Occurrence in Acutely Ill Patients Investigators Sepsis Occurrence in Acutely Ill Patients Investigators Sepsis in European intensive care units: results of the SOAP study. Critical Care Medicine342, 344-353.
Zafer MM, Al-Agamy MH, El-Mahallawy HA, Amin MA, Ashour SD. 2015.Dissemination of VIM-2 producing Pseudomonas aeruginosa ST233 at tertiary care hospitals in Egypt, BMC Infectious Diseases 15, 122. DOI 10.1186/s12879-015-0861-8.
Zakaria EA. 2005. Increasing Ciprofloxacin resistance among prevalent urinary tract bacterial isolates in Gaza Strip, Palestine. Journal of Biomedicine and Biotechnology (3), 238-241. DOI:10.1155/JBB.2005.238.
Shahida Mangi, Anwar Hussain Phulpoto, Muneer Ahmed Qazi, Nisar Ahmed Kanhar (2016), Antibiotic resistance pattern and gender wise prevalence of Pseudomonas aeruginosa strain isolated from the tertiary health care units; IJB, V9, N5, November, P173-182
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